Block 1

Question 1

DnaA Protein

Answer 1

binds to origin of replication and breaks H bonds at 9mer, strand separation at 13mer

Question 2

DnaC

Answer 2

delivers helicase to DNA template

Question 3

DnaB

Answer 3

opens helix and binds primase to form primosome protein complex

Question 4

The fact that bacteria can make many proteins from a single mRNA

Answer 4

Polycistronic

Question 5

4 things eukaryotic mRNA have that prokaryotic do not

Answer 5

Inrons, exons, 5’ cap, Poly a tail

Question 6

Eukaryotic mRNA that is not modified yet is called

Answer 6

nascent mRNA

Question 7

The role of the 5’ cap on mRNA

Answer 7

Assist with processing, transport, prevents degradation, promote translation

Question 8

Enzyme adds a GMP to the 5’ end of eukaryotic mRNA in a reverse linkage (5’-5’)

Answer 8

Guanylyl transferase

Question 9

Adds a methyl group to the 2’-O-position on the next to last base on the 5’ end

Answer 9

2’-O-methyl transferase

Question 10

A methyl group is added to the 7 position of the terminal guanine of mRNA

Answer 10

Guanine-7 methyl transferase

Question 11

One phosphate group is removed from the 5’ end of eukaryotic mRNA

Answer 11

Phosphatase

Question 12

This is the only polymerase enzyme that produces capped mRNA’s

Answer 12

RNA polymerase II

Question 13

What protein binds to the 5’ splice junction?

Answer 13

U1 snRNP

Question 14

What protein binds to the branch point sequence?

Answer 14

U2 snRNP

Question 15

What happens after the lariat is formed?

Answer 15

Intron is excised by cleavage at the 3’ splice junction and exons 1&2 are ligated, snRNP’s released

Question 16

What enzyme edits the apo-B mRNA in mammalian cells?

Answer 16

cytidine deaminase

Question 17

Apo-B100

Answer 17

liver

Question 18

Apo-B48

Answer 18

intestine (premature stop codon CAA -> UAA)

Question 19

When a weak splice site within an intron creates a secondary (cryptic) splice site this is known as

Answer 19

Internal splice site

Question 20

mRNA processing proteins such as capping, splicing and polyadenylation factors are found where?

Answer 20

On the tail of RNA polymerase II

Question 21

Spliceosome is activated

Answer 21

U4 dissociates