Biotechnology Flashcards
Define methylases
Enzymes that modify a restriction enzyme recognition site by adding a methyl group to a base.
Define vector
Delivery system moves foreign dna into a cell.
What are vectors chosen based off of?
Size and sequence of foreign DNA fragments, characteristics of the cells, and goal of transformation.
What is the goal of genetic transformation?
Express gene product. Change the traits of the organism that receives the foreign dna.
Define transgenic
Cell or organism transformed by dna from another species.
What are plasmids?
Small, circle, double stranded dna molecules.
Define intragenic/cisgenic.
A gene moved within the same species or closely related species.
Define subgenic.
Edited gene to amplify, delete, insert, silence, or repress the gene.
Why would a gene need to be repressed?
When the product it creates is not currently needed, so it will use up energy. **Turning off*
What is a segment of dna that is added to another sequence called?
Insert.
