Biotechnology Flashcards
IT COMES FROM THE LATIN WORD MUTATIONEM, MUTO, MEANING “ I MOVE” OR “ I CHANGE”
Mutation
IT REFERS TO ANY PERMANENT CHANGE IN THE NUCLEOTIDE SEQUENCE OR IN THE GENETIC MATERIAL AS A RESULT OF A FAILURE OF THE SYSTEM, WHICH CAN BE COMPARED AS A TYPOGRAPHICAL ERROR IN AN INSTRUCTIONAL MATERIAL
IT CAN ALSO BE HEREDITARY AND THESE CHANGES CAN ALSO BE BENEFICIAL & HARMFUL.
Mutation
are a type of mutation where one or a few nucleotides of a gene are affected.
Small-scale mutation
___is when a nucleotide is substituted with a different nucleotide and is sometimes referred to as point mutation, i.e. when the gene mutation involves only one nucleotide
Substitution Mutation
A ___ is substituted with a different purine (A → G)
Purine
A ___ replaced by a different pyrimidine (C → T)
Pyrimidine
What are the 5 types of point mutations?
Frameshift mutation, Nonsense mutation, Missense mutation, Neutral mutation, & Silent mutation
is a type of gene mutation wherein the addition or deletion of (a number of) nucleotide(s) causes a shift in the reading frame of the codons in the mRNA.
Frameshift mutation
a form of mutation producing a nonsense codon. A nonsense codon, as the name implies, does not code for amino acid and it leads to a protein product that is early truncated.
Nonsense mutation
It results in a codon that specifies a different amino acid, and thus, causes the synthesis of a protein with an altered amino acid sequence during translation.
Missense mutation
It has no selective advantage or disadvantage.
Neutral mutation
Is when the codon codes for the same amino acid and therefore produces neither structural nor physiological change in the protein product.
Silent mutation
is a chromosomal mutation, e.g. deletion, inversion, or amplification of a region of a chromosome.
Large-scale mutation
It is the process of using recombinant DNA (rDNA) technology to alter the genetic makeup of an organism.
a. Genetic Engineering
b. Biotechnology
c. Chemical Engineering
d. Cross breeding
a. Genetic Engineering
It is a technique used to separate DNA fragments according to their sizes.
a. Polymerases
b. Gel electrophoresis
c. DNA Ligase
d. Restriction enzymes
b. Gel electrophoresis