Biotechnology

Question 1

Tools necessary for recombinant DNA technology

Answer 1

Restriction enzymes
Cloning Vectors and
Component host

Question 2

The first restriction endonuclease

Answer 2

Hind II

Question 3

The convention of naming enzymes

Answer 3

First letter (E) of the genus
Two letters (co) of the species
The first letter of the strain(R)
The order in which the enzyme was isolated from the strain of bacteria (I)
Eg: EcoRI from Escherichia coli RY 13

Question 4

Nucleases are of two types

Answer 4

Exonuclease and endonuclease

Question 5

Each restriction enzyme recognizes a specific ______ nucleotide sequence

Answer 5

Palindromic

Question 6

The sticky ends of two complementary fragments are joined by

Answer 6

DNA ligase

Question 7

The fragments formed can be separated by a technique

Answer 7

Gel electrophoresis

Question 8

Explain the process of gel electrophoresis

Answer 8

The negatively charged DNA fragments are moved towards the anode under an electric field through medium. Commonly used medium is agarose gel. The DNA fragments separate according to their size . The separated fragments are visualized by staining with ethidium bromide and exposure to UV to form orange DNA fragments

Question 9

The process of extraction of DNA from the gel is known as

Answer 9

Elution