Biotech

Question 1

Father of genetic engineering

Answer 1

Paul Berg

Question 2

First restriction endonuclease to isolate was

Answer 2

HindII

Question 3

Metals used in gene gun

Answer 3

Gold and tungsten 1-2 micrometres

Question 4

The first plasmid used in Recombinant DNA

Answer 4

Pbr 322 buy Boliver and Rodriguez

Question 5

Who developed polymerase chain reaction

Answer 5

Kary mullis

Question 6

Who got the Nobel Prize for discovery of restriction endonuclease

Answer 6

Aber

Smith and Nathan

Question 7

Who coined the term biotechnology

Answer 7

Karl erkey

Question 8

Tobacco nematode

Answer 8

Meloidogyne incognita

Question 9

An rnase is enzyme that cuts double stranded DNA into small interfering RNA

Answer 9

Dicer

Question 10

What silences

Mrna

Answer 10

Antisense RNA
SiRNA + dicer ribonuclease form RNA induced silencing complex

RISC that binds to the complementary mRNA

Question 11

First transgenic variety to reach market

Answer 11

Flavr savr

Question 12

First transgenic animal obtained for food production

Answer 12

Genetically modified Salman

Question 13

Who first extracted pure insulin

Answer 13

Banting and best from a dog

Question 14

Total amino acids in insulin

Answer 14

51 to change A and B

A has 21 amino acids and B has 30 amino acids which are drawn Together by disulfide bridges

Question 15

Recombinant therapeutics worldwide that have been approved

Answer 15

30

Question 16

Recombinant therapeutics available in India

Answer 16

12

Question 17

RNA interference takes place in prokaryotes or eukaryotes

Answer 17

Eukaryotes