Biosphy CCM Pyrenoids

Question 1

what are pyrenoids

Answer 1

semi-crystalline protein aggregates found in most green alage

Question 2

observed when

Answer 2

1803

Question 3

function discovered when

Answer 3

1970s

Question 4

they are what to carboxysomes

Answer 4

analogues to carboxysomes allow turbocharging

Question 5

active uptake of HCO3 from the environment what pumps?

Answer 5

HCII

HCAI

Question 6

HCO3 is pumped into?

what genes/transporter

Answer 6

chlorplast mem

CCP1
CCp2
HCAI

Question 7

Pyrenoids are also found in what species

Why might they be present here?

Answer 7

Hornworts

Thought may be present due to wetness, extra barrier CO2 diff

Question 8

when fully induced carboysome CCM what happens to Ki

Answer 8

Ki decreases - higher affinity

Question 9

Hornworts, sister group to vascular plants, have pyrenoids in ….. of their …… species

Answer 9

50% of 200 hornwort species

Question 10

Engineer cyanobacterial carboxysome

step 1.

Answer 10

1. bicarbonate transporters e.g StbA +BicA pumps + Na/H+ pumps to maintain pH Nha53

Question 11

Engineer cyanobacterial carboxysome

step 2.

Answer 11

Carboxysomes with their conjugate rubisco itroduced

Question 12

Engineer cyanobacterial carboxysome

step 3.

Answer 12

remove CA from chloroplast + stoma + endogenous rubisco