Biosafety and Risk Assessment Flashcards
Application of knowledge, techniques & equipment to prevent personnel, laboratory and environmental exposure to potentially infections agents or biohazard
Biosafety
Principle of Biosafety
Containment
things to have to build Mol Bio Lab
- Safe Methods
- Facilities
- Equipment
Used in describing for managing infectious materials in the laboratory environment where they are being handled or maintained
containment
Persons working with infectious agents or potentially infected materials must be:
aware of potential hazards
must be trained
proficient in the practices and techniques required for handling such material safely
Risk Consequences: Severe
Fatality
Risk Consequences: Insignificant
No injury
Risk Consequences: Minor
Injury/ill health requiring first aid
Risk Consequences: Moderate
Injury/ill health requiring medical attention
Risk Consequences: Major
Injury/ill health requiring hospital admission
Risk Likelihood: Rare
May occur somewhere, sometime (“once in a lifetime/once in a hundred years)
Risk Likelihood: Unlikely
May occur somewhere w/in the Department over an extended period of time
Risk Likelihood: Possible
May occur several times across the Department or a region over a period of time
Risk Likelihood: Likely
May be anticipated multiple times over a period of time
May occur once every few repetitions of the activity or event
Risk Likelihood: Almost Certain
Prone to occur regularly
It is anticipated for each repetition of the activity of event
Risk Level/Rating: Extreme
Notify Workplace Manager &/or Management OHS Nominee immediately. Corrective actions should be taken immediately. Cease associated activity.
Risk Level/Rating: High
Notify Workplace Manager &/or Management OHS Nominee immediately. Corrective actions should be taken within 48 hours of notification.
Risk Level/Rating: Medium
Notify Nominated employee, HSR/HSC. Nominated employee, OHS representative/HSC is to follow up that corrective action is taken within 7 days.
Risk Level/Rating: Low
Notify Nominated employee, HSR/HSC. Nominated employee, HSR/HSC is to follow up that corrective action is taken within a reasonable time.
It kills bacterias and viruses by inhibiting the action of enzymes
Diluted Bleach
Denatures protein substances
Ethyl alcohol/Ethanol
time to clean using bleach
10-20 minutes
Triple application for cleaning
- 10% bleach
- Ethanol
- UV light
what to use if you don’t know what spillage is on the surface
water
neutralizers
spill kits
Potentially infectious materials must be placed in
a durable, leak proof container during
○ collection
○ handling
○ processing
○ storage
○ transport within a facility
inward airflow protects worker
CLASS I
exhaust to outside (with or without
HEPA filter)
CLASS I
protects worker, product, environment used with aerosol-transmissible microorganisms, tissue culture
CLASS II
objective of containment
confine biohazardous and to reduce the potential exposure of the laboratory worker, persons outside of the laboratory, and the environment to potentially infectious agents
Waste Management: Green bin
General waste: Kitchen waste, paper and tissues, and water bottles and cans
Waste Management: Red bin
Infected plastics: Syringes, gloves, plastic waste
Waste Management: Yellow bin
Infected waste: soiled, anatomical, chemical liquid, cytotoxic, laboratory waste, expired and discarded medicines
Waste Management: Blue bin
Glassware: antibiotic vials, metallic implants, glassware material, expect cytotoxic
Waste Management: White bin
Sharps: Needles and cut glasses
Color of the bin for sharps non-infectious
white
Color of the bin for sharps infectious
red
Waste Management: Secured land filling
Green and red
Waste Management: Deep burial
Yellow
Waste Management: Re-cycler
Blue
Waste Management: Sharp pit
White
inward airflow protects worker
CLASS I
protects worker, product, environment used with aerosol-transmissible microorganisms, tissue culture
CLASS II
exhaust to outside (with or without HEPA filter
CLASS I
these cabinets are further differentiated by types based on construction, airflow and exhaust systems
CLASS II
safety equipment
Personal Protection
Biosafety cabinets
Facility Security Plans
