Biosafety Flashcards
What is the difference between Risk Group & Containment Level?
Risk Group: The relative hazard posed by an infectious agent in the lab.
Containment Level: The precautions needed perform a particular technique with a particular agent in order to protect lab workers/public/environment.
What are the requirements for a Containment Level 1 lab?
Lab Design:
- An open benchtop
- Sink
Equipment:
- “Standard PPE:”
- Gloves
- Lab coat
- Eye protection
Practices:
- “Standard microbiological practices:”
- Avoid splashes + aerosols.
- Safe sharps handling.
- Mechanical pipetting.
- Decontaminate work surfaces.
What are the requirements for a Containment Level 2 lab?
Lab Design:
- Biohaz signs
- Autoclave
Equipment:
- BSC I or II
Practices:
- Limited access
- Extra sharps precautions
- Respiratory protection, as required
- Biosafety manual with policies on decontam + medical surveillance
What are the requirements for a Containment Level 3 lab?
Lab Design:
- Physically separated from the main access corridors.
- Double doors, self-closing.
- NEGATIVE pressure airflow into the lab.
- Do not recycle lab exhaust air.
Equipment:
- Respiratory protection as needed.
Practices:
- Controlled access.
- Decontaminate waste + clothes.
- Store serum specimens of lab personnel.
What are the requirements for a Containment Level 4 lab?
Lab Design:
- In a separate building, or an isolated zone.
- Dedicated air supply+exhaust vacuum, and a decontamination system.
- UV light rooms, + autonomous systems to detect + destroy pathogens.
Equipment:
- BSC III,orNSC I/II plusfull-body positive-pressure personnel suit with its own air supply.
Practices:
- Change clothes before entering.
- Shower + decontaminate all material on exit.
Name 5 Risk Group 3 organisms
- Bacillus anthracis
- Brucella
- Burkholderia (pseudo)mallei
- Botulinum toxin
- Chlamydia psittaci (avian strains)
- Francisella tularensis (type A)
- MTB/M. bovis (non-BCG)
- Pasteurella multocida
What Risk Group is Bacillus anthracis?
RG3
What Risk Group is Brucella?
RG3
What Risk Group is Burkholderia mallei/B.pseudomallei?
RG3
What Risk Group is Clostridium botulinum?
RG3
What Risk Group is Chlamydophila psittaci?
RG3, for avian strains.
What Risk Group is Francisella tularensis?
RG3
(type A) {}
What Risk Group is Mycobacterium tuberculosis?
RG3
What Risk Group is Mycobacterium bovis?
RG3
(non-BCG)
What Risk Group is Pasteurella multocida?
RG3
