Biosafety Flashcards
The application of knowledge, techniques
and equipment to prevent personnel,
laboratory and environmental exposure to
potentially infectious agents or biohazards.
Biosafety
A fundamental objective of any biosafety in
molecular biology in laboratory programs is
the ___________ of potentially harmful
biological agents.
containment
The term “containment” is used in
describing
➢ Safe methods
➢ Facilities
➢ Equipment
For managing infectious materials in the laboratory
environment where they are being handled or
maintained
Persons working with infectious agents or
potentially infected materials must be…
➢ Aware of potential hazards
➢ Must be trained
➢ Proficient in the practices and techniques required for handling such material safety
Enumerate Risk Group Classification…
Risk Group 1: No or low individual and community risk
Risk Group 2: Moderate individual risk, low community risk
Risk Group 3: High individual risk, low community risk
Risk Group 4: High individual and community risk
What are the definition of these Risk Consequences?
- Insignificant: Level 1
- Minor: Level 2
- Moderate: Level 3
- Major: Level 4
- Severe: Level 5
Definition
1.No injury
2.Injury/ill health requiring first aid
3.Injury/ill health requiring medical attention
4.Injury/ill requiring hospital admission
5.Fatality
what is the descriptor of these Risk level rating
- Notify Workplace Manager and/or Management OHS Nominee immediately. Corrective actions should be taken immediately. Cease associated activity
- Notify Workplace Manager and/or Management OHS Nominee Immediately. Corrective actions should be taken within 48 hours of notification
- Notify Nominated employee, HSR/HSC.
Nominated employee, OHS representative/HSC is to follow up that corrective action is taken within 7 days. - Notify Nominated employee, HSR/HSC. Nominated employee, HSR/HSC is to follow up that corrective action is taken within a reasonable time
- Extreme
- High
- Medium
- Low
Enumerate what are those Standard Laboratory Practices
● Bags, lab coats, books etc. should be
placed at specified locations
● Food facilities and eating (NO FOOD OR
DRINK ALLOWED)
● Prohibited Mouth pipetting
● Washing hands
● Surface and Equipment Decontamination
● The work area should be cleaned and
maintained in sanitary condition
● Surfaces or equipment where work with
biological materials is conducted should be
routinely decontaminated.
this solution kills bacterias and viruses by inhibiting the actions of enzymes.
10% Diluted Bleach
this solution denatures protein substances that
could contaminate DNA and RNA materials
Ethyl Alcohol / Ethanol
70% and 90%
When applying bleach, let it sit for ________
10-15 minutes
The importance of pest management in the laboratory lies in its role in maintaining a clean and controlled environment
Insects can be contaminated and may lead
to false positive results.
What is the rationale behind labeling laboratory apparatus?
Most reagents are colorless therefore it’s
important to read the label to prevent mixing
why should there be a door for access control in every laboratory
Laboratories often house valuable
equipment, sensitive experiments, and
confidential information. Access control
doors help prevent unauthorized individuals
from entering restricted areas, ensuring the
security of the laboratory and its assets.
List the categories for segregating items in the laboratory.
- Green: General Waste - paper, tissues, water bottles etc.
- Red: Infected plastics - syringes, gloves. and plastic waste
- Yellow: Infected waste - soiled, anatomical, chemical liquid, cytotoxic, laboratory waste, expired & discarded medicines
- Blue: glassware - antibiotic vials, metallic implants, glassware material, expect cytotoxic
- Clear: sharps - needs and glasses
