Biosafety Flashcards

1
Q

The application of knowledge, techniques
and equipment to prevent personnel,
laboratory and environmental exposure to
potentially infectious agents or biohazards.

A

Biosafety

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2
Q

A fundamental objective of any biosafety in
molecular biology in laboratory programs is
the ___________ of potentially harmful
biological agents.

A

containment

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3
Q

The term “containment” is used in
describing

A

➢ Safe methods
➢ Facilities
➢ Equipment
For managing infectious materials in the laboratory
environment where they are being handled or
maintained

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4
Q

Persons working with infectious agents or
potentially infected materials must be…

A

➢ Aware of potential hazards
➢ Must be trained
➢ Proficient in the practices and techniques required for handling such material safety

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5
Q

Enumerate Risk Group Classification…

A

Risk Group 1: No or low individual and community risk
Risk Group 2: Moderate individual risk, low community risk
Risk Group 3: High individual risk, low community risk
Risk Group 4: High individual and community risk

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6
Q

What are the definition of these Risk Consequences?

  1. Insignificant: Level 1
  2. Minor: Level 2
  3. Moderate: Level 3
  4. Major: Level 4
  5. Severe: Level 5
A

Definition

1.No injury
2.Injury/ill health requiring first aid
3.Injury/ill health requiring medical attention
4.Injury/ill requiring hospital admission
5.Fatality

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7
Q

what is the descriptor of these Risk level rating

  1. Notify Workplace Manager and/or Management OHS Nominee immediately. Corrective actions should be taken immediately. Cease associated activity
  2. Notify Workplace Manager and/or Management OHS Nominee Immediately. Corrective actions should be taken within 48 hours of notification
  3. Notify Nominated employee, HSR/HSC.
    Nominated employee, OHS representative/HSC is to follow up that corrective action is taken within 7 days.
  4. Notify Nominated employee, HSR/HSC. Nominated employee, HSR/HSC is to follow up that corrective action is taken within a reasonable time
A
  1. Extreme
  2. High
  3. Medium
  4. Low
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8
Q

Enumerate what are those Standard Laboratory Practices

A

● Bags, lab coats, books etc. should be
placed at specified locations
● Food facilities and eating (NO FOOD OR
DRINK ALLOWED)
● Prohibited Mouth pipetting
● Washing hands
● Surface and Equipment Decontamination
● The work area should be cleaned and
maintained in sanitary condition
● Surfaces or equipment where work with
biological materials is conducted should be
routinely decontaminated.

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9
Q

this solution kills bacterias and viruses by inhibiting the actions of enzymes.

A

10% Diluted Bleach

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10
Q

this solution denatures protein substances that
could contaminate DNA and RNA materials

A

Ethyl Alcohol / Ethanol
70% and 90%

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11
Q

When applying bleach, let it sit for ________

A

10-15 minutes

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12
Q

The importance of pest management in the laboratory lies in its role in maintaining a clean and controlled environment

A

Insects can be contaminated and may lead
to false positive results.

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13
Q

What is the rationale behind labeling laboratory apparatus?

A

Most reagents are colorless therefore it’s
important to read the label to prevent mixing

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14
Q

why should there be a door for access control in every laboratory

A

Laboratories often house valuable
equipment, sensitive experiments, and
confidential information. Access control
doors help prevent unauthorized individuals
from entering restricted areas, ensuring the
security of the laboratory and its assets.

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15
Q

List the categories for segregating items in the laboratory.

A
  1. Green: General Waste - paper, tissues, water bottles etc.
  2. Red: Infected plastics - syringes, gloves. and plastic waste
  3. Yellow: Infected waste - soiled, anatomical, chemical liquid, cytotoxic, laboratory waste, expired & discarded medicines
  4. Blue: glassware - antibiotic vials, metallic implants, glassware material, expect cytotoxic
  5. Clear: sharps - needs and glasses
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16
Q

What are the settings for your autoclave
Materials & Disposal

A

Materials - 121 degrees Pressure: 15 for 15
mins
Disposal - 132 degrees for 15 mins

17
Q

Potentially infectious materials must be
placed in a durable, leak proof container
during…

A

➢ Collection
➢ Handling
➢ processing
➢ Storage
➢ Transport within a facility (Triple
pathway system)

18
Q

What are the safety equipments

A

● Personal Protection (PPE)
● Safety Cabinets (biosafety cabinet)
● Facility Security Plans

19
Q

Why is it necessary for every medical technologist to be vaccinated?

A

● The use of vaccines may provide an
increased level of protection.

20
Q

All manipulations of infectious materials
within the laboratory must be conducted in
the ___________

A

Biological safety cabinet

21
Q

What are the features of a Class I biosafety cabinet?

A

● Inward airflow protects worker
● Exhaust to outside (w/wo HEPA filter)

22
Q

What are the different types of Class II biosafety cabinets?

A

Class II type A1 - has the tendency to have
a circulation of our air (that is 70%)
recirculated air and 30% of it is exhausted

Class II type B1 - totally 100% exhausted

23
Q

Most common biosafety cabinet used in
laboratory?

A

CLASS II - TYPE A2

24
Q

What is the feature of a Class III biosafety cabinet?

A

All entry of air is filtered by HEPA filters

25
Q

Why is it essential to have an incident, accident, and emergency response plan in the laboratory?

A

This guide provides both emergency and
nonemergency telephone numbers

26
Q

As much as possible it should be present in the laboratory

A

First aid boxes

27
Q

What are spill kits for?

A

Spill kits are designed to contain, control, and clean up hazardous spills such as chemicals or acids.

28
Q

If acid is spilled use..

A

Basic or alkaline

29
Q

If alkaline is spilled use..

A

Acid

30
Q

What are the requirements for fire prevention and fire protection?

A

● Fire alarm system
● Fire detection system
● Fire doors in good order

31
Q

What is the main objective of containment?

A

to confine biohazards and to reduce the potential exposure of the laboratory worker, persons outside of the laboratory, and the environment to potentially infectious agents