biomolecules Flashcards

1
Q

what are carbohydrates???????

A
  • many MONOSACCHARIDES combined chemically to form POLYSACCHARIDES which are complex macromolecules such as starch, glycogen and cellulose
  • primary source of energy
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2
Q

what test to use for carbohydrates???????

A

Benedicts solution

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3
Q

steps for benedicts solution :(

A
  1. add 1.0cm2 of test solution into a clean, dry test tube
  2. add 1.0cm2 of Benedicts solution to the test tube and swirl
  3. leave the test tube in boiling water bath for 3 minutrd
  4. observe for the formation of precipitate
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4
Q

results for benedicts solution :)

A

remains blue - not present
green - ‘ trace’
yellow- ‘ low’
orange - ‘ “ moderate”
brick red - present

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5
Q

what are proteins ??????

A
  • contains hydrogen, nitrogen, oxygen and sometimes sulfur
  • made up of primary units called amino acids that are linked via peptide bonds to form polypeptides, joint together to form protein
  • important for growth and repair
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6
Q

test for proteins :(

A

bluret test

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7
Q

steps for bluret test :(((((

A
  1. add 1.0cm2 of solution in a dry, clean tube
  2. add 1.0cm2 of blurent REAGENT to the test solution. shake to mix
  3. shake well and observe any colour change
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8
Q

results for blurets test :))))))))))

A

mixture turns from blue to violet/ lilac - protein present
mixture remains blue - protein absent

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9
Q

what are fats?

A

-carbon, hydrogen, oxyen
- glycerol and three fatty acid molecules
- stores energy and heat insulataor

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10
Q

fat molecule =?

A

triglyceride

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11
Q

test for fats

A

ethanol emulsion test

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12
Q

steps for ethanol emulsion test

A
  1. add 2cm2 of ethanol to two drops of solution in a test tube. mix well and allow it to stand for 2 minutes
  2. decant the ethanol into another test tube with 2cm2 water. mix well
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13
Q

results for ethanol emulsion test

A

white emulsion formed - fats are present
remains clear - fats absent

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14
Q

starch breakdown

A

starch molecule (amylase) -> maltose molecule (maltase) -> glucose molecules

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15
Q

what are enzymes?

A
  • found in living things
  • made out of protein
  • fold to take on a 3D globular shape
  • they function as biological catalysts. they catalyse or speed up the rate of chemical reactions. they remain chemically unchanged at the end of the reaction
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16
Q

TWO purposes? of enzymes

A
  1. build up complex substances
    - cells build up or synthesise complex substances from complex ones (eg.amino acids to polypeptide to protein)
  2. break down complex substances
    - cells break down complex substances to simpler substances (eg. starch molecule to glucose molecules)
17
Q

naming of enzymes??

A

according to scientific system, shows substance where its from?? ends with “ase”

18
Q

three types of enzymes? (hint:CAtPRotectsLIves)

A
  1. carbohydrases: enzymes that digest carbohydrates
  2. proteases: enzymes that digest proteins
  3. lipases: are enzymes that digest fats (lipids)