biomolecules Flashcards
what are carbohydrates???????
- many MONOSACCHARIDES combined chemically to form POLYSACCHARIDES which are complex macromolecules such as starch, glycogen and cellulose
- primary source of energy
what test to use for carbohydrates???????
Benedicts solution
steps for benedicts solution :(
- add 1.0cm2 of test solution into a clean, dry test tube
- add 1.0cm2 of Benedicts solution to the test tube and swirl
- leave the test tube in boiling water bath for 3 minutrd
- observe for the formation of precipitate
results for benedicts solution :)
remains blue - not present
green - ‘ trace’
yellow- ‘ low’
orange - ‘ “ moderate”
brick red - present
what are proteins ??????
- contains hydrogen, nitrogen, oxygen and sometimes sulfur
- made up of primary units called amino acids that are linked via peptide bonds to form polypeptides, joint together to form protein
- important for growth and repair
test for proteins :(
bluret test
steps for bluret test :(((((
- add 1.0cm2 of solution in a dry, clean tube
- add 1.0cm2 of blurent REAGENT to the test solution. shake to mix
- shake well and observe any colour change
results for blurets test :))))))))))
mixture turns from blue to violet/ lilac - protein present
mixture remains blue - protein absent
what are fats?
-carbon, hydrogen, oxyen
- glycerol and three fatty acid molecules
- stores energy and heat insulataor
fat molecule =?
triglyceride
test for fats
ethanol emulsion test
steps for ethanol emulsion test
- add 2cm2 of ethanol to two drops of solution in a test tube. mix well and allow it to stand for 2 minutes
- decant the ethanol into another test tube with 2cm2 water. mix well
results for ethanol emulsion test
white emulsion formed - fats are present
remains clear - fats absent
starch breakdown
starch molecule (amylase) -> maltose molecule (maltase) -> glucose molecules
what are enzymes?
- found in living things
- made out of protein
- fold to take on a 3D globular shape
- they function as biological catalysts. they catalyse or speed up the rate of chemical reactions. they remain chemically unchanged at the end of the reaction