Biomolecules Flashcards
G.N ramchandran discovered
Tripal Helical Structure
All carbon compounds that we get from living tissue are called
Biomolecules
Elements that shows respective content per unit mass of living tissue
Carbon, Hydrogen, Oxygen ,Earth Crust
Elements that differ in relative abundance than in Earth Crust
carbon and Hydrogen
How to analyse chemical composition ?
Living organism have both organic and inorganic compounds in them
How to analyse organic compounds?
Take living tissue and grind in Trichloroacetic acid using mortar and pestle which form slurry.
Filter slurry through cheesecloath or cotton obtain
Micromolecule & Macromolecule
Examples of organic, inorganic compounds found in acid soluble pool
Water, mineral,amino acids,sugar and nucleotide
Biomicromolecule present in
Acid soluble
18 to 1000 Da found in
Acid soluble Biomicromolecule
Biomacromolecule found in
Acid insoluble pool
1000 Da and above are found in
Biomacromolecule
Examples of biomacromolecule
Protein, polysaccharides, nucleic acids
Molecular weight of lipids are not exceed above
800Da
Exception of biomacromolecule which are less than 1000 Da
Lipid
Lipid come under acid insoluble fraction because
Lipid form vesicles which are not water soluble
Vesicles are not
Water soluble
Lipid are arrenge into
Structure like cell membrane
Membrane fragments in the form of vesicles get separated along with acid insoluble pool because
On destruction of cell membrane the vesicles are form and they are not water soluble
Lipid are not strictly
Macromolecules
What separation techniques we can use to identify perticular compound for other compound using
Chromatography, Centrifugation, Filtration
Analytical technique give us idea about
Mile formula and Portable structure of compund
After burning dry living tissue we get
All the carbon compounds that oxidised to gaseous form CO2 and Water Vapour get removed
Ash of living tissue contain inorganic elements like
Ca, Mg , Sulphate , Phosphate
Acid soluble pool represents
Cytoplasmic Composition
Weight of oxygen in Earth Crust and Human body is
Earth Crust - 46.6 and
Human body - 65.00
How many charector take part in composition of protoplasm
34
Only __ elements are main or universal element in protoplasm
13
Which elements are 96% major part of protoplasm
C,H,O,N
Remaining elements of protoplasm in trace quantity are
Trace elements
Carbon constitute more than __% of dry matter
50%
Who are essential organic compounds
Metabolites
Metabolites formed in
Living organism as a result of metabolic activity
Types of Metabolites
1.Primary Metabolites
2. Secondary Metabolites
Intermediate and product of normal vital metabolic pathway are known as
Primary Metabolites
Who are essential for growth, development And Reproduction of living organism
Primary Metabolites
Primary Metabolites direct involved in
Fundamental metabolic processes
Production of primary Metabolites matches
Growth of population
Animal Tissue are only
Primary Metabolites
Example of primary Metabolites
Amino acids, Protein, Enzymes, Hormones
Plant tissue and Microbe have additional metabolites known as
Secondary Metabolites
Secondary metabolites produce at
Only at Perticular growth phase
Who has no direct involvement in fundamental metabolic processes
Secondary Metabolites
Secondary metabolites production usually after
Main growth phase of plant or microbe
Secondary metabolites formed by
Alterations of normal or primary Metabolic pathway
Examples of secondary Metabolites
Alkaloid, flavonoids,Scents,gum, coloured pigment, antibiotics
Examples of secondary metabolites
- Pigment - Carotenoids,Anthoctanis
2- Alkaloid - Morphine, Codeine - Essential oil - lemon grass oil
4.Toxin - Abrin ,Ricin
5.Lectins- Concanavalin A
6.Drugs - Vinblastins ,Curcumin
7.Polymeric substances - Rubber,Gums, Cellulose
Ratio of H & O is
2 :1
What are hydrates of carbon
Compound of C,H,O with ratio 2:1
All carbohydrates are
Polyhydroxy aldehyde or Ketone
In body 55% of energy is obtained from
Carbohydrates diet
Classification of carbohydrates are based on
Sugar units and the complexity
Who can’t be further hydrolyzed into simpler carbohydrates
Monosaccharide
Six members rings are known as
Pyranose
5 membered ring are known as
Furanose
Monosaccharide have
Crystalline structure, Sweet teste and Soluble in water
All monosaccharide are
Reducing sugars
Monosaccharide classification based on
No of carbon atoms
Oligosaccharides are formed by
Condensation of 2-10 monosaccharide
Oligosaccharides classification is based on
No of monosaccharide