Biology Unit 4 Model Answers Flashcards
D- Test cross
Testing a suspected heterozygote by crossing it with a known homologous recessive
M- What is reproductive isolation
when gametes are incompatible - different number of chromosomes so fusion cannot occur
When members of the species no longer recognise each other - different characteristics
When courtship behaviour is different
M- Mark release recapture estimate of population size
Capture large sample of organisms
Mark with non toxic paint/ not too obvious to predators/ wont wash up
Count and release
Allow time to disperse throughout population
Recapture large sample
Count total and how many are marked
Population = N1 x N2/ NM in 1st
M- Explain how carbon dioxide is produced during aerobic respiration
One CO2 is released during the link reaction when pyruvate 3C is converted t acetyl coA 2C
2 molecules of CO2 are released during the Kreb’s cycle when the 6C compound is decarboxylated to regenerate the 4C compound
M- Synthesis of ATP during the electron transport chain (oxidative phosphorylation
Takes place on the inner mitochondrial membrane/cristae
Reduced NAD and FAD are oxidised
Electrons passed from one carrier to another in an electron transport chain ETC
As electrons pass down the chain, they lose energy which is dissipated as heat
Hydrogen ions are pumped into the intermembrane space
Hydrogen ions diffuse back into the matrix down an electrochemical gradient
Via ATP synthase
ATP is made from ADP and Pi
Electrons at the end of the transport chain bind with H+ ion and are accepted by oxygen, the final acceptor, to make water
M- When you are asked to comment on a demographic transition graph what must you refer to?
life expectancy
M- How do you decide the number of quadrats to use in order to collect representative data?
Large number of quadrats so results are more reliable with repeats
Enough to be able to carry out a statistical test - SR requires a minimum of 7 pairs
Not too many for the available time
Calculate a running mean which changes little when there are enough quadrats.
M- Impact of inefficiency of energy transfer
Most food chains only have 4-5 trophic levels because insufficient energy is available to support a large breeding population at the top level
Biomass is less at higher trophic levels
Total amount of energy stored in each level is less at higher trophic levels
M - Factors affecting death rate
Age profile/ life expectancy at birth/ food supply/ effective sanitation/ medical care/ natural disasters/ war
M- what are the limitations of collecting plant data as percentage cover?
They may overhang where the leaves of larger plants are outside the quadrat
Smaller plants may be difficult to count where overshadowed by larger plants
M- Population pyramids
Stable population- birth rate and death rate in balance; no increase or decrease in population size
Increasing population- high birth rate; gives a wider base to the pyramid; fewer older people ; so narrower apex
Decreasig popualtion - lower birth rate; narrower base; more older people; woder apex
M- Pattern of population growth curves
Period of slow growth as small numbers reproduce slowly - lag phase
Period of rapid growth - expontential/log phase - population doubles per unit of time
Period where population growth remains stable with cyclic fluctuations
M - effect of predator - prey relationship on population size
Predators eat prey and reduce the prey poplation
Predators now in greater competition for food
Predators population is reduced as some individuals cannot compete
Fewer prey are eaten
Prey population increases
More prey for food so predator population increases
M- Adaptations of the leaf
Large surface area to absorb light
Arrangement of leaves that avoids shadowing
Thin so short diffusion pathway
Transparent cuticle and epidermis to allow light through to mesophyll
Lots of chloroplasts in upper mesophyll cells
Numerous stomata for gas exchange
Air spaces in lower mesophyll for rapid diffusion
Network of xylem and phloem
M- Describe how carbon in carbon dioxide becomes carbon in triose phosphate
Carbon dioxide combines with RuBP to make 2x G3P. This is reduced to TP which requires NADPH to provide the hydrogen for reduction and energy from the breakdown of ATP
M- Information required to calculate growth of a population
Birth and deaths
Number of emigrants and immigrants
M- Flow of energy through an ecosystem
Light energy converted to chemical energy during photosynthesis
Organic molecules converted to ATP during respiration
ATP used by cells to perform useful work
M- Roles of ATP
Energy released in smaller manageable quantities
Hydrolysis of ATP to ADP in a single step reaction so releases immediate energy
M- How NADP is reduced
NADP accepts a hydrogen and electron from ohtlysis/breakdown of water using light energy
M- Adaptations of chloroplast to the light independent reaction
Fluid of stroma contains enzymes needed to carry out reduction carbon dioxde
Stroma fluid allows easy diffusion of products of light dependent reaction into the stroma
Contains DNA and ribosomes to make proteins quickly
M- Describe how the light dependent reactions produce ATP and reduced NADP
Chlorophyll absorbs light energy
Electrons in chlorophyll are excited/energised by light energy/photons
This increases the energy levels in the electrons
Electrons move to an electron acceptor
Electrons lose energy as they are passed from one electron carrier to the next along the electron transfer/transport chain (ETC)
As electrons are passes along the ETC, ATP is formed from ADP and Pi using energy from electrons (photophosphorylation)
NADPH is formed when the electrons from the electron transfer chain and H+ from photolysis combine with NADP
NADP + H+ –> NADPH
The H+ and electron come from photolysis - to reduce NADP to NADPH
O2 is given off and the electrons replace those lost from chlorophyll
Photolysis - the break down of water using light energy
H2) –> @H+ + 1/2O2 + 2e-
M-Decarboxylation in respiration
Pyruvate - a 3 carbon compound - loses a carbon atom with co enzyme A, to produce a 2 carbon compound of acetyl coenzyme A
During this conversion decarboxylation occurs and CO2 is released
Acetyl co enzymes A then enters the Krebs cycle and combines with a 4C compound to produce a 6 carbon compound
The 6 C compound is then converted to a 5C compound and decarboxylation occurs
This 5C compound is then converted to a 4C compound and decarboxylation occurs again releasing CO2. NAD and FAD are reduced to give NADH and FADH2
M- Adaptations of the chloroplast to the light dependent reaction
Thylakoid membrane provide a large surface area for the attachment of chlorophyll, enzymes and electron carriers
Network of proteins hold chlorophyll in a position to absorb maximum amount of light
Granal membranes have enzymes that help makes ATP
Chloroplasts have their own DNA and ribosomes to make proteins quickly
M- Describe the part played by the inner mitochondrion in producing ATP
Cristae contain electron transport proteins that transfer electrons from the carriers NADH and FADH
H+ ions from the carriers are pumped into the intermembrane space using energy lost by electrons
H+ ions move back into the matrix along a concentration gradient by ATPase
Energy from the H+ ion gradient is used to combine ADP and Pi to make ATP
M- How does an increase in carbon dioxide levels in the atmosphere affect yield of grain?
Causes an increase in rate of photosynthesis therefore increases the amount of glucose made which increases growth rate - due to an increases rate of respiration and there ATP prodction- and yield
Increase in carbon dioxide levels usually causes an increase in climate temperature which would cause faster reaction rate hence higher yield
M- Significance of Krebs cycle
breaks down macromolecules into smaller ones
Produces hydrogen atoms that are carried by NAD to the ETC for oxidative phosphorylation (and the subsequent production of ATP)
Regenerates the 4C compound which would otherwise accumulate
Source of intermediate compounds used in the manufacture of other substances
M- How does the concentration of carbon dioxide in a forest change over time?
Low carbon dioxide during the day
As plants are photosynthesising and taking in carbon dioxide
Plants respire all the time but do not photosynthesise at night so carbon dioxide concentrations increase at night
Carbon dioxide levels are increased at the forest floor
Because ground level has less photosynthesising tissue, less light and more respiring animals
M- Glycolysis
Glucose is activated - phosphorylated - by the addition of inorganic phosphate
Produced by the breakdown of ATP into ADP and Pi
Glucose splits into 2 molecules of triose phosphate
NAD co enzyme accepts hydrogen from triose phosphate
and becomes reduced
Inorganic phosphorus is removed from triose phosphate to make pyruvate
4 molecules of ATP are produced by substrate level phosphorylation to give a net gain of 2 ATP per glucose molecule
M- Effective pesticides should be…
Specific
Biodegradeable
cost effective
will not accumulate in the food chain
M- Link reaction
Pyruvate is oxidised to the 2C compound acetyl coA
CO2 is released
NAD accepts hydrogen from pyruvate and so is reduced
M- Krebs cylce
2C acetyl coA combines with 4C compound
6C compound loses CO2
5C compound loses CO2
One molecule of ATP is produced by substrate level phosphorylation
FAD and NAD accept hydrogen and electrons
And becomes reduced (FADH and NADH)
4C compound combines with another 2C compound and the cycle begins again
M- Why is it important that samples are collected at random?
To avoid bias
M- The true rate of photosynthesis is greater than simply the plants uptake of carbon dioxide, why?
Some of the carbon dioxide produced in respiration is used in photosynthesis
M- Light independent reaction
Carbon dioxide joins with ribulose bisphosphate (RuBP) a 5 carbon compound- this gives an unstable 6 carbon compound
This quickly breaks down to 2 molecules of a 3 carbon compound glycerate 3-phosphate (GP)
This reaction is catalysed by the enzymes ribulose bisphosphate carboxylase (Rubisco)
ATP from the light dependent reaction is used to provide energy for the reduction of GP into TP - triose phosphate
The reduction reaction needs H+/reducing power
H+ are provided by reduced NADP (NADPH)from the light dependent stage
Two TP join up to form hexose which can combine to form starch and cellulose, 1 in every 6 TP molecules is used to make hexose
TP regenerates RuBP and so the cycle continues, 5 in every 6 TP molecules form RUBP
TP also forms amino acids, fatty acids, glycerol, DNA, RNA, glucose and starch.
M- Fermentation in yeast
C6H12O6 –> 2CH3CH2OH + CO2 ( glucose –> ethanol and carbon dioxide)
Anaerobic conditions
Optimum temperatures/pH
Only glycolysis can take place
Glucose –> pyruvate (NAD –>NADH)
Pyruvate –> Ethanol + CO2 (NADH –> NAD)
Nad is recycled/regenerated
M- What happens to pyruvate in anaerobic conditions and why this type of respiration is advantageous to skeletal muscle
Pyruvate takes up hydrogen ions from the reduced NAD
To produce lactate and NAD
This ensures that glucose can still be respired
Even in the absence of oxygen
M- Name inorganic nitrogen containing compounds
Nitrates
Nitrites
Ammonium
M- Advantages of mitochondria having more cristae
Larger surface area to carry out oxidative phosphorylation producing more ATP
M- Role of coenzymes and carrier proteins in synthesis of ATP
NAD accepts hydrogen during glycolysis, link reaction and Kreb’s cycle
NAD is reduced
NADH releases hydrogen ions during the electron transport chain, which are pumped across the mitochondrial membrane
Electrons released from the co enzymes are passed along a series of carrier proteins in the membrane
Energy lost from the electrons is used to make ATP from ADP and Pi
M- Why does a reduction in temperature decrease the amount of carbon dioxide in respiration?
Enzymes catalyse respiration reactions
Rate of reaction decreases as particles have less kinetic energy
So fewer collisions between enzyme active site and substrate
So fewer ES complexes can form
M- Explain why an increase in temperature leads to a decrease in the rate of respiration
Respiration is catalysed by enzymes which are proteins
Hydrogen bonds in the tertiary structure break
Active site is denatured
Substrate can no longer bind to the active site so fewer ES complexes can form
M- Nitrogen cycle
Plants take up nitrate ions (NO3^-) from the soil
The nitrates then form amino acids which are used to synthesise proteins which are organic nitrogen containing compounds in plants and animals
The plants are eaten by animals and the proteins are digested
Both plants and animals die, leaving a collection of dead materials (detritus) which contains nitrogen containing compounds
Decomposers decay the excretory products and detritus, releasing ammonia (NH3) and ammonium ions (NH4^+) into the soil
Nitrifying bacteria oxidise the ammonia into nitrites (NO2^-)
Then the nitrites are oxidised into nitrates which are taken up by the plants
Lightning and nitrogen fixing bacteria in the soil and in nodules on the roots of legumes fix nitrogen gas (N2) into ammonia (NH3) which is dissolved into ammonium
Denitrifying bacteria reduce nitrate to nitrogen, in anaerobic soil conditions that escape from the soil and returns to the atmosphere
M- Why does anaerobic respiration produce more carbon dioxide than aerobic respiration
Anaerobic respiration produces less ATP so more glucose is respires to generate the same amount of ATP
M- What happens when the rate of respiration is greater than the rate of photosynthesis?
The plant will be using more glucose than it is producing. Therefore the plant will not grow as quickly because there is less ATP produced for reactions such as DNA replication and protein synthesis - this will have an impact of total plant biomass and therefore lower yield
M- Why a low percentage of energy is transferred along the food chain
Some of the organism is not eaten
Some parts are not digested
Some energy is lost in excretory material e.g. urine
energy is lost as heat from the organisms
Energy is lost in repsiration
D- Abiotic
The non-living part of the environment
M- why intensive rearing increases energy conversion rate
Movement is restricted therefore less energy used in muscle contraction
Warm environment to reduce heat loss from the body
No wastage in feeding as animals receive optimum amount
Predators are excluded
M- Limitations of using pyramids of numbers
No account is taken of size of the organism
Number of individuals may be so great as to not be able to represent them accurately
M- Why organisms need energy
Metabolism
Movement - energy for muscle contractions
Active transport - change shape of carrier proteins
Cell division
Production of enzymes or hormones - energy needed to form lysosomes for secretion
Maintenance of internal body temperatures
M- Describe how and explain why the efficiency of energy transfer is different at different stages in the transfer
Some light is reflected off the plant or is the wrong wavelength to be absorbed by chlorophyll
Efficiency of photosynthesis is low - less than 2%
energy lost during respiration/excretion or some parts of the plants/animal are not eaten
Heat loss
efficiency f transfer increases with increasing trophic level (along food chain) so efficiency of consumers is greater than efficiency of producers
Efficiency is lower in older animals, primary consumers, warm blooded animals (endotherms)
Carnivores use more of their food than herbivores
M- Deforestation
Loss of trees means less photosynthesis
Therefore less CO2 removed from atmosphere
Unwanted trees often burned releasing CO2 into the atmosphere
The concentration of CO2 in the atmosphere increases leading to increased global warming
Reduced input to nitrogen cycle as less recycling of nitrate ions occurs
Increases loss of nitrates by leaching
roots of trees hold fertile part of soil= topsoil together. With no trees, wind erosion occurs causing removal of topsoil
Soil loses fertility so can only support lower numbers and fewer species of plants, giving lower biodiversity
D- Abundance
The number of individuals of a species in a given place
M- Factors that affect net productivity
The efficiency of the crop
The area of ground covered by the leaves of the crop
M- Disadvantages of chemical psticides
always have some effect on non target species
must be re applied therefore expensive
pests may develop resistance
D- Percentage Cover
An estimate of the area within a quadrat that a particular plant species covers