Biology Paper One Flashcards
What’s the difference between prokaryotes and eukaryotes?
Eukaryotic Cells: Complex e.g animal/plant cells
Prokaryotic Cells: Simple and Smaller e.g bacteria
Describe bacterial cells?
- They don’t have a true nucleus
- Contain small rings of DNA called plasmids
- Bacteria don’t have mitochondria
What’s the equation for magnification?
image size ÷ real size
How do you prepare a slide? Onions
- Add a drop of water to the middle
- Cut up an onion and separate it into layers. Use tweezers to peel off some epidermal tissue for the bottom.
- Using tweezers, place that tissue into the water.
- Add some iodine solution.
- Place a cover slip on top.
What is meant by differentiation?
Differentiation: the process by which a cell changes to become specialised for its job.
How are sperm cells specialised?
- It has a long tail and a streamlined head to help it swim
- There are lots of mitochondria to provide energy
- Carries enzymes to break down the eggs cell membrane
How are nerve cells specialised?
- The cells are long (to cover more distance)
- Branched connections at their ends to connect to other nerve cells
How are muscle cells specialised?
- Cells are long so they have space to contract
- Lots of mitochondria to generate the energy needed for contractions.
How are root hair cells specialised?
- Large surface area for absorbing water and minerals from the soil
How are phloem and xylem cells specialised?
- Cells are long and joined end-end
- Xylem cells are hollow
- Phloem cells have very few subcellular structures so things can flow through them
Explain growth and mitosis?
- DNA doubles are forms an x-shaped chromosome.
- Chromosomes line up at the centre, the two duplicates go to opposite sides.
- Membranes form around each set and become the nucleus.
- The cytoplasm and membrane split.
- Two identical daughter cells are made.
How do prokaryotic cells replicate?
Binary Fission
- The circular DNA and plasmids replicate.
- The cell gets bigger and the circular DNA strands move to opposite sides of the cell.
- The cytoplasm begins to divide and new cell walls begin to form.
- Cytoplasm divides and two daughter cells are produced.
What is diffusion?
Diffusion: the spreading out of particles from an area of higher concentration to an area of low concentration.
What is osmosis?
Osmosis: the movement of water molecules across a partially permeable membrane from a region of higher water concentration to a region of lower water concentration.
What is a catalyst?
A substance which increases the speed of a reaction without being changed or used up in the reaction.
Every enzyme has an active site that fits onto the substance involved in the reaction.
What conditions do enzymes need?
- Generally 37 degrees, if they get too hot they denature.
- optimum pH level, normally pH of 7.
How do you convert carbohydrates into simple sugars?
Starch →amylase enzyme→maltose
Amylase is made in: the salivary glands, the pancreas and small intestine.
How do you convert proteins into amino acids?
Proteins→protease enzyme→amino acids
Proteases is made in: stomach, pancreas and small intestine.
How do you convert lipids?
Lipid→lipase enzyme→Glycerol and Fatty Acids
Lipases are made in: the pancreas and small intestine.
What is bile?
Bile: neutralises the stomach acid and emulsifies fats.
- Bile is produced in the liver and stored in the gall bladder.
- The hydrochloric acid makes it too acidic, bile is an alkaline so it neutralises it.
What are the different types of pathogens?
Bacteria, viruses, protists and fungi
What are viruses?
- They’re not cells
- The reproduce rapidly
- The live inside a host cell and use it to replicate
- The cell with then burst and release all the copies
- The cell damage makes you feel ill
What are protists?
- They’re all eukaryotes, most of them are single-celled
- Some protists are parasites, they live in or on other organisms.
- They are often transferred to the organism by a vector
What is fungi?
- Some fungi are single-celled
- Others have a body, which is made up of hyphae
- These can grow and penetrate human skin or the surface of plants
- The hyphae can produce spores
How can pathogens be spread?
Water
Air
Direct contact
What are measles?
- Spread by droplets
- Develops a red skin rash and fever
- Can be very serious, sometimes fatal
- Most people are vaccinated against it
What is HIV?
- A virus is spread by sexual contact or exchanging bodily fluids (sharing needles)
- Causes flu-like symptoms
- Attacks immune cells
- Damages the immune system
What is TMV?
Tobacco Mosaic Virus
- Virus that affects plants
- Causes a mosaic pattern on the leaves, becomes discoloured
- Reduces photosynthesis
What is Malaria?
`- Carried by a vector (mosquitoes)
- Causes repeating episodes of fever
- People can be protected by insecticides and mosquito nets.
What are 2 examples of bacterial diseases?
Salmonella: - Causes food poisoning - Symptoms are fever, cramps, vomiting and diarrhoea - Toxins are released Gonorrhoea: - STD - Pain when they urinate, yellow/green discharge - Originally treated with antibiotics
What are some body defence systems?
- Skin is a barrier
- Hairs and mucus trap particles
- Trachea and bronchi secrete mucus and are lined with cilia.
- Stomach produces hydrochloric acid
What are the 3 lines of attack for pathogens?
1, Consuming them - WBC engulf and digest them (phagocytosis)
- Producing Antibodies - The pathogens have antigens, the WBC recognise these as foreign and begin to produce antibodies to lock onto these. These then kill the pathogens.
- Producing antitoxins - These counteract the toxins produced by bacteria.
What are the 3 main stages in drug testing?
- Preclinical testing - drugs are tested on human cells and tissues.
- Preclinical testing - tested on animals to see if the drugs are toxic and the dosage.
- Clinical trial - it is firstly tested on healthy humans to make sure it has no harmful sound effects. Then they are testing on volunteers suffering from the illness. Tested with a group, some are given the drug and others are given a placebo.
What are monoclonal antibodies?
They are identical antibodies. Antibodies are produced by B-lymphocytes ( a type of WBC). They are produced from lots of clones of a single WBC. Tumour cells don’t produce antibodies but they divide lots. You can fuse a B-lymphocyte with a tumour cell to create a cell called a hybridoma. These cells can be cloned.
How are monoclonal antibodies used in pregnancy tests?
If you’re pregnant:
- The hormone binds to the antibodies on the blue beads.
- The urine moves up the stick, carrying the hormone and the beads.
- The beads and hormone bind to the antibodies on the strip.
- So the blue beads get stuck on the strip, making it blue.
If you’re not pregnant:
- The urine still moves up the stick, carrying the blue beads.
- There’s nothing to stick the blue beads onto so it doesn’t go blue.
How can monoclonal antibodies be used to treat diseases?
- Different cells in the body have different antigens on the surface, so you can make monoclonal antibodies that bind to specific cells.
- Cancer cells have antigens on their cell membrane called tumour markers.
- In the lab they make monoclonal antibodies that bind to the tumour markers.
- An anti-cancer drug can be attached to these monoclonal antibodies.
- These are given to the patient through a drip.
- The antibodies target specific cells.
- The drug only kills the cancer cells.
What is the equation for photosynthesis?
carbon dioxide + water → glucose + oxygen
How do plants use glucose?
- Respiration
- Making Cellulose
- Making Amino Acids
- Stored as oils or fats
- Stored as Starch
What are limiting factors for photosynthesis?
- Light
- Carbon Dioxide
- Temperature
What is respiration?
Respiration: the process of transferring energy from glucose, which goes on in every cell.
What is meant by metabolism?
Metabolism: all the chemical reactions in an organism.
What is the equation for anaerobic respiration in plants and yeast?
Glucose → Ethanol + Carbon Dioxide
How can embryonic cells be used to cure diseases?
1) Make insulin-producing cells for people with diabetes
2) Nerve cells for people with paralysed by spinal injuries
What is the maximum temperature at which microorganisms in a school lab should be grown?
25 degrees
What are the 2 main differences between active transport and diffusion?
Diffusion: high concentration to low
Active transport: low concentration to high
Diffusion doesn’t require energy, active transport does.
What does it mean when an enzyme has been denatured?
When the shape of the enzymes active site changes so it can’t fit properly
What is the role of alveoli?
Carry out gas exchange
Why is the circulatory system is described as a ‘double circulatory system’?
Two circuits are joined together.
First circuit: right ventricle pumps deoxygenated blood to the lungs to take in oxygen, the blood then returns to the heart.
Second circuit: left ventricle pumps oxygenated blood around all the organs in our body, the deoxygenated blood then returns to the heart.
What is the role of the vacuole?
Contains cell sap which helps maintain rigidity of the cell (only in plants)
Anaerobic respiration in humans?
Glucose –> lactic acid + CO2 and energy.
How small is a bacteria cell compared to a regular body one?
1/100th of the size.
Why does bacteria presence in your body make you feel ill?
They produce toxins that damage cells and tissue.
How large is a virus compared to a bacterium?
1/100th of the size (smaller).
How does a virus replicate?
It inserts its DNA into a cell and uses its machinery to create copies.
Why does a virus make you feel ill?
Cell damage occurs during reproduction using a host cell as it ‘bursts’ open.
What are pathogens?
Micro-organisms that cause infectious disease.
How do you calculate rate?
amount of products / time
How do you calculate percentage change?
difference between percentages / old percentage x 100
How do you calculate the percentage of a factor?
amount / total x 100
How do you use a light microscope to view your slide? PRACTICAL
- Clip the side onto the stage
- Select the lowest-power objective lens
- Use the coarse adjustment knob to move the stage up or down.
- Look down the eyepiece
- Adjust focus with the fine adjustment knob
- For greater magnification use a higher-powered objective lens.
Which of the following subcellular structures would you not expect to find in a prokaryotic cell?
- Plasmid
- Nucleus
- Cell Wall
- Cell Membrane
Nucleus
What is the function of a chloroplast?
Absorbs light which is needed for photosynthesis
What are the differences between adult stem cells are embryo stem cells?
- Adult stem cells are found in the bone marrow
- Can only turn into certain cells eg red blood cells
- Embryo stem cells are found in embryos and can turn into any type, they can also be grown in a lab to produce clone cells.
Why are some people against stem cell research? (4)
- Unethical
- Curing patients who already exist and are suffering is more important
- Scientists should focus on developing other sources rather than embryos
4 Cell research is banned in some countries
How are stem cells used to produce identical plants?
- Stem cells are found in the meristems
- These tissues can differentiate at any time
- Produce clones quickly and cheaply
- Can be used to produce more plants of a rare species
- Can also be used to grow crops of identical plants that have desired features for farmers eg. disease resistance
Describe the structure of chromosomes
Chromosomes are coiled up lengths of DNA molecules. Each chromosome carries a large number of genes, these control the development of different characteristics. Body cells have 2 copies of each chromosome.