Biology Lab Exam 2 Flashcards

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1
Q

Micropipette

A

a very fine pipette for measuring, transferring, or injecting very small quantities of liquid.

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2
Q

“Don’ts” of Micropipetting

A
  • Don’t use a micropipette without a tip
  • Don’t put a loaded pipette with liquid on a flat surface
  • Don’t allow the plunger to snap back after expelling liquid
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3
Q

Gel electrophoresis

A

method of separating macromolecules via a gel matrix (agarose)

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4
Q

Agarose Gel

A

gel mixture used for electrophoresis, polysaccharide made into gel

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5
Q

loading dye in gel electrophoresis

A
  • increases the density of the DNA sample (allows them to sink through the gel well)
  • allows the bands to be seen
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6
Q

TAE Buffer

A
  • maintains the pH of the gel (DNA is sensitive to pH changes)
  • gives the DNA a liquid to “swim” through
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7
Q

Restriction Endonucleases Work

A
  • Restriction enzymes cut DNA based on the DNA sequence

- Usually 4-6 bases serve as the recognition site and as the restriction “cut” site

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8
Q

Photosynthesis lab

A
  • sodium bicarbonate solution acted as the CO2 in the equation
  • the leaf would float due to O2 being made (from photosynthesis) which causes the leaves in the experiment to become buoyant
  • yellow light causes the leaves to float
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9
Q

light-dependent reactions

A

use energy from light to produce ATP and NADPH

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10
Q

Where does photosynthesis occur?

A

in the thylakoid (Chloroplast)

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11
Q

endonucleases

A

enzymes that cut RNA or DNA at specific sites; restriction enzymes are endonucleases that cut DNA

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12
Q

Bacteriophage

A

attacking bacteria

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13
Q

palindrome

A

sequence of DNA that reads the same forwards and backwards

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14
Q

Lambda

A

cut pieces of DNA of different sizes

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15
Q

What two molecules create light dependent reactions?

A

ATP and NADPH

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16
Q

Photosynthesis equation

A

6CO2 + 6H2O +light energy ——> C6H12O6 + 6O2 + 6H2O

17
Q

Calvin Cycle

A

reactions of photosynthesis in which energy from ATP and NADPH is used to build high-energy compounds such as sugars