biology - enzymes Flashcards
1
Q
how to investigate the effect of pH on the rate of reaction of the amylase enzyme?
A
- place starch solution, amylase solution and buffer solution into a water bath at 25°C for a few minutes
- combine solutions and add a drop of the mixture to iodine solution on a tile
- add the mixture to iodine solution drops on the tile in 30 second intervals until the iodine solution remains brown
- calculate the rate of reaction: 1 / time taken
- repeat at different pH values using different buffer solutions
- plot graph of rate (y axis) against pH (x axis)
2
Q
which enzyme catalyses the breakdown of starch in the human digestive system?
A
amylase - catalyses breakdown of starch to maltose
3
Q
why must samples be placed in a water bath before being mixed?
A
to allow temperature to equilibrate (control variable)
4
Q
what is a buffer solution?
A
a solution which can resist changes in pH
