Biology, Ecology & Bio-tech Flashcards
Explain Carbohydrates
- Carbohydrates are organic compounds made of carbon, hydrogen, and oxygen atoms.
- a) In food science and informal contexts, the term carbohydrate often just means any sugary food like chocolate or a starchy food such as bread or pasta.
- The most basic carbohydrates are simple sugars or monosaccharides such as fructose (C6H12O6), which makes fruit sweet, or ribose (C5H10O5), which forms the backbone of the genetic molecule RNA.
- a) Monosaccharides, especially glucose, are also the major source of fuel for metabolism. Glucose has the same chemical formula as fructose but a different, typically ring-shaped, molecular structure.
- b) The larger disaccharide sucrose (C12H22O11)—common table sugar—is formed from fructose and glucose.
- The most complicated carbohydrates are “polysaccharides,” including starch, which is made up of thousands of glucose units.
- Plants store their glucose fuel as starch. Many animals, including humans, store glucose as glycogen, a molecule containing a core protein surrounded by many branching glucose units.
Explain Protein
- Proteins are large, complex molecules that play many critical roles in cells.
- They consist of long chains of hundreds or thousands of smaller simple molecules called amino acids. Some of these are “essential amino acids,” such as phenylalanine, which our bodies don’t synthesize, so it’s essential that we eat plenty of these in our diet.
- Some proteins act as antibodies, which can prevent disease by targeting foreign particles such as viruses and blocking the sites they use to invade cells.
- a) Others include receptors and enzymes, which enable thousands of chemical reactions to take place in cells and assist the construction of new proteins by reading the genetic information in DNA.
- In plants and animals, all proteins are made from different sequences of twenty main amino acids.
- The exact sequence for a given protein is called its primary structure.
- When a cell makes a new protein, it forms a linear chain of amino acids that coils into a secondary structure before morphing into a final three-dimensional shape through a process called protein folding.
Explain Lipids
- Lipids are a broad family of molecules including fats, waxes, and some vitamins (including vitamins A, D, E, and K) that are hydrophobic—they repel water and are only soluble in organic solvents such as acetone.
- Lipids have a wide range of biological functions, including storing energy, maintaining cell membranes and acting as hormones that are able to coordinate complicated processes like fertility
- Common lipid types include fats, steroids, and phospholipids.
Fats store energy and cushion organs, protecting them from damage, and are composed of fatty acids and glycerol, a sweet-tasting alcohol.
Steroids contain four ring-shaped hydrocarbon molecules and include the dietary fat cholesterol as well as the sex hormones estradiol and testosterone.
- Phospholipids usually contain two fatty acids and a phosphate group.
In water, they arrange themselves into a two-layered sheet with all their hydrophobic tails lined up in the middle. This layered structure forms cell membranes, which regulate the flow of ions and molecules in and out of a cell.
Explain Metabolism
- Metabolism describes the host of chemical reactions required to keep living organisms alive, generating energy for essential growth and reproduction—for instance, healing injuries and eliminating toxins.
- Apart from water, most molecules in living organisms are amino acids, the building blocks of proteins, as well as carbohydrates and lipids. Metabolic reactions involving them divide into two categories.
“Anabolism” builds molecules like proteins during construction of new cells and tissues,
while “catabolism” breaks down molecules from food for use as a source of energy.
- Enzymes also play a vital role in metabolism by acting as catalysts that efficiently transform one chemical into another, helping amino acids join to form proteins, for instance, or breaking down dietary starch into its component sugars.
- Healthy metabolism in humans depends on good nutrition, plentiful water, and exercise. A lack of any one of these decreases your metabolic rate and can lead to weight gain.
Explain Chemosynthesis
- Chemosynthesis is the process by which some exotic microbes living in hot deep-sea vents derive their energy. It’s similar to photosynthesis, but doesn’t use sunlight.
Instead, the energy comes from the oxidation of inorganic chemicals such as hydrogen sulfide bubbling up from the Earth’s crust.
- In hydrothermal vents, geothermal heat coming up from fissures on the ocean floor can heat water to more than 212°F (100°C).
Amazingly, some bacteria called extremophiles thrive in these vents at temperatures up to about 250°F (120°C).
There’s no sunlight available, so the bacteria produce their energy by turning available chemicals into sugar. For instance, some bacteria oxidize hydrogen sulfide and use the energy stored in its chemical bonds to make glucose from water and carbon dioxide dissolved in seawater.
Scientists speculate that these bacteria would have been well adapted to the hot conditions on the early Earth, making them a good candidate for one of the earliest types of life.
Explain Receptors
- In biochemistry, a receptor is a protein molecule in the membrane or cytoplasm of a cell onto which signaling molecules such as hormones attach to deliver chemical instructions.
For example, the hormone insulin regulates blood sugar by latching onto a receptor in muscle or liver cells, triggering reactions that speed up sugar absorption.
Signaling molecules effectively target specific receptors because they have the right size, shape, and electric charge distribution to grab hold of them, a bit like a key fitting into a lock.
The binding “unlocks” the cell to cause chemical changes. Many drugs mimic signaling molecules to promote their effect.
For instance, morphine mimics endorphins, naturally occurring feel-good chemicals in the body that relieve pain.
Other drugs lock onto receptors purely in order to block available binding sites, inhibiting the effects of natural signaling molecules.
Examples include antihistamines, which alleviate allergies by inhibiting chemicals called histamines that cause rashes, sneezing, and itching.
Explain DNA
- Deoxyribonucleic acid, or DNA, is the molecule that encodes the genetic instructions for the development and function of all living, self-replicating organisms.
- Nearly every cell in a person’s body has the same DNA, which is mostly inside the cell nucleus, although some of it resides in mitochondria.
3. The information in DNA is stored as a sequence of four chemical “bases” called adenine (A), guanine (G), cytosine (C), and thymine (T).
3.a) The bases team up (A with T; C with G) to form units called base pairs.
Human DNA consists of about 3.2 billion base pairs. Each base is attached to a sugar molecule called deoxyribose and a phosphate molecule to form a nucleotide.
- The nucleotides are arranged in two long strands that form a double helix shaped like a spiraling ladder, with the base pairs forming the vertical supports.
- DNA replicates itself by splitting into single strands that serve as templates for duplicating the sequence of bases.
Explain RNA
- Ribonucleic acid or RNA is a molecule that has some similarities to DNA but does not store genetic information (except in RNA viruses). Instead, RNA performs many different functions in a cell, acting as a temporary copy of genetic information, for instance.
- Like DNA, RNA molecules have a sequence of four chemical “bases,” in this case uracil (U), adenine (A), cytosine (C), and guanine (G).
- a) Each base is also attached to a sugar molecule (ribose) and a phosphate molecule to form a nucleotide. The bases sometimes team up with each other (U with A; C with G) to form a double helix structure like DNA,
**but RNA usually occurs as a single strand.
- “Messenger” RNA (mRNA) is a short-lived molecule that copies a cell’s DNA and carries it to the cell’s protein synthesis machinery, the ribosome, which reads off its information to make the right protein.
- “Transfer” RNA (tRNA) molecules latch onto individual amino acids and frogmarch them to the ribosome, where they are incorporated into proteins.
Explain Genes
- Genes are segments of DNA that act as a blueprint for the production of specific proteins.
- b) They exist in alternative forms called alleles, which determine the distinct traits that parents pass on to their offspring according to the laws of inheritance.
- The entirety of an organism’s genetic information is called the genome.
- The thirteen-year Human Genome Project, completed in 2003, was a mammoth international effort to identify the sequences of the 3.2 billion base pairs in human DNA and identify its approximately twenty-five thousand genes.
- a) This showed that the average gene consists of about three thousand base pairs, but sizes vary enormously, with the largest gene having 2.4 million base pairs.
- b) The Human Genome Project has clarified the role that certain gene sequences play in diseases, including breast cancer and muscular dystrophy.
However, it also revealed that only about 2 percent of the genome actually encodes instructions for protein synthesis, and the role of some of the remaining DNA remains a mystery.
Explain laws of inheritance
- The laws of inheritance are basic rules about how animals and plants pass on traits to their offspring.
- Gregor Mendel, a nineteenth-century Austrian monk, discovered the laws in pea-breeding experiments, in which he cross-fertilized pea plants and studied traits like flower color and the length of plant stems in subsequent generations.
- a) Mendel’s experiments revealed that two factors (now known to be genes) determined the traits, one from each parent, and if the two inherited factors are different, the offspring expresses just one of them, the so-called “dominant” trait.
- b) He also noticed that different traits like flower color and stem length are inherited independently.
- Today we know that all the human blood groups (A, AB, B, or O) are determined by a single gene. A and B are dominant while O is “recessive,” so a child who inherits A + O or B + O from its parents will have blood types A and B respectively. A and B are said to be “co-dominant,” so that inheriting both A and B gives blood type AB.
Explain Prokaryotes
- Prokaryotes are simple single-celled organisms that don’t have a cell nucleus that houses DNA.
- a) Instead, their DNA forms a free-floating bundle in the middle of the cell. Like eukaryotic cells, prokaryotes have ribosomes where amino acids are assembled into proteins, and sometimes a flagellum—a rudder-like tail that propels them.
- The fossil record reveals that prokaryotes evolved on Earth very early, at least 3.5 billion years ago. They reproduce asexually and are typically about 1–10 micrometers (millionths of a meter) wide.
- They fall into two subcategories: bacteria and archaea.
- a) Bacteria were discovered in the late 1600s and are ubiquitous in every habitat on Earth. They have a wide range of shapes, including spheres, spirals, and rods.
- b) Archaea were first classified as a separate group in the late 1970s. Most look similar to bacteria but they have a completely different genetic and biochemical make-up, and often inhabit extreme habitats such as scorching hydrothermal vents on the ocean floor.
Explain Eukaryotes
- Along with prokaryotes, eukaryotes are one of two major cell types—they make up everything from single-celled amoebas to complex animals and plants.
- A typical size for a eukaryotic cell is roughly 0.01 mm across—about ten or fifteen times wider than a typical prokaryote.
- The “plasma membrane” forms the outer barrier of a eukaryotic cell, while the cell nucleus houses DNA packaged into chromosomes that vary widely between organisms.
- Humans have twenty-three pairs of large, linear chromosomes.
The nucleus is surrounded by a water-rich fluid called cytosol and various organelles that perform different tasks. - Mitochondria generate energy, while the endoplasmic reticulum is a network of interconnected membranes dotted with ribosomes, which assemble proteins.
- Fossil evidence suggests eukaryotes evolved at least 1.7 billion years ago. One possibility is that they arose because some prokaryotic cells engulfed others, which were not digested but lived on as organelles and reproduced.
Explain Mitochondria
- Mitochondria act as the power plants of eukaryotic cells, converting the energy from food into a form that cells can use. A cell may have hundreds or even thousands of mitochondria depending on its energy demands.
- Mitochondria are like little factories that specialize in using energy from reactions between oxygen and simple sugars to produce molecules of adenosine triphosphate (ATP), the cell’s main energy source.
- a) ATP is a bit like a charged battery—removal of a phosphate group releases energy to drive complex reactions, leaving “uncharged” adenosine diphosphate (ADP), which returns to mitochondria to be recharged into ATP.
- Surrounded by two membranes, mitochondria have their own genetic material and reproduce independently of their host cell.
- Scientists suspect that the ancient ancestors of mitochondria were probably free-living bacteria that somehow became engulfed in other cells. The bacteria thrived in the protective environment of their new host cells, while the hosts came to rely on the bacteria for energy production.
Explain Ribosomes
- In all cells, including those of plants, animals, and bacteria, ribosomes are the mini factories that assemble proteins.
- Each ribosome is built from RNA molecules and proteins. Some of them are free to roam in the watery cytoplasm of a cell, while others are bound to the endoplasmic reticulum, a complicated network of interconnected membranes inside eukaryotic cells.
- A strand of messenger RNA (mRNA) brings a copy of genetic information from a cell’s DNA to a ribosome. Meanwhile, transfer RNA (tRNA) molecules latch onto single amino acids and deliver them to the ribosome, to be incorporated into proteins according to the mRNA’s instructions.
- Cells typically have several thousand ribosomes, but the number can reach several million.
- The chemical structure of ribosomes is different in bacteria and animal cells, and the differences allow many antibiotic drugs to selectively disrupt the ribosomes of disease-causing bacteria, sabotaging their protein production without making people or animals sick.
Explain cell division
- Cell division is the process by which biological cells multiply.
- Eukaryotic cells, including human cells, create identical copies of themselves for growth or repair of tissues through a process called mitosis.
- The double-stranded DNA in the cell nucleus unzips into two strands that each join with nucleotides to form two copies of the original DNA.
In the next step, cytokinesis, the cell splits into two identical copies of the original cell.
- Meiosis is a type of cell division that creates eggs and sperm for sexual reproduction. The eggs and sperm cells have half the normal number of chromosomes. When sperm fertilizes an egg, they fuse to regain the normal chromosome quota, half from the male and half from the female.
- Prokaryotic cells such as bacteria usually divide in a process called binary fission.
The single DNA bundle in a prokaryotic cell replicates, then the two copies stick to different parts of the cell membrane, going their separate ways when the cell splits in two.