Biology-aseptic techniques Flashcards
1
Q
Why do we use aseptic techniques?
A
to prevent contamination from bacterias and to stop the risk of infection
2
Q
What are inoculating hoops/needles for?
A
They are used to transfer a bacterial monoculture to a medium without the risk from contaminating organisms
3
Q
Where is the hottest part of the flame?
A
The tip of the cone
4
Q
What is the shape of bacterial colonies
A
A circle
5
Q
What is the zone of inhibition?
A
A circular area around the spot of the antibiotic in which the bacteria does not grow
6
Q
Why is the neck of the flask heated?
A
To destroy any bacteria that may be there
7
Q
Name three aseptic techniques?
A
- Heat the inoculating hoop
- Hold the lid of the petri dish over the petri dish so no air borne contaminants can fall on it
- Hold the neck of the flask over the tip of the cone of the flame for a few seconds
8
Q
What is the medium used when culturing microorganisms?
A
Agar jelly
