Biological Molecules Glossary Flashcards
Amino Group
Each amino acid has the same fundamental structure, which consists of a central carbon atom which is bonded to an amino group (NH2), a carboxyl group (COOH) and to a hydrogen atom.
Benedict’s test
the test for reducing sugars,
- Approximately 1 ml of sample is placed into a clean test tube.
- 2 ml (10 drops) of Benedict’s reagent (CuSO4) is placed in the test tube.
- The solution is then heated in a boiling water bath for 3-5 minutes.
- Observe for colour change in the solution of test tubes or precipitate formation.
A positive result is indicated by a brick-red colouration.
Biuret test
the test for protein, it detects peptide bonds,
- at room temperature, place a sample of the solution to be tested in a test tube and add an equal volume of sodium hydroxide.
- add a few drops of very dilute (0.05%) Copper (II) Sulfate and mix gently.
- A purple colouration indicates the presence of peptide bonds, if no protein is present the solution remains blue.
Carboxyl group
Each amino acid has the same fundamental structure, which consists of a central carbon atom which is bonded to an amino group (NH2), a carboxyl group (COOH) and to a hydrogen atom.
Condensation reaction
The reaction that takes monomers and bonds them to form polymers - H2O given off
Disaccharide
2 bonded monosaccharide molecules
Disulphide bonds
disulphide bonds are formed by the oxidation of two cysteine residues to form a covalent sulfur-sulfur bond
Emulsion test
Test for lipids:
- Take a completely dry and grease-free test tube.
- Add 2cm^3 of the sample being tested, add 5cm^3 of ethanol.
- Shake the tube thoroughly to dissolve any lipid in the sample.
- Add 5cm^3 of water and shake gently.
- A cloudy-white colour indicates the presence of a lipid.
- As a control, repeat the procedures using water instead of the sample, the final solution should be clear.
Fructose
A monosaccharide, a sweet tasting soluble substance with a general formula of C6H12O6
Galactose
A monosaccharide, a sweet tasting soluble substance with a general formula of C6H12O6
Glucose
A monosaccharide, a sweet tasting soluble substance with a general formula of C6H12O6
Glycerol
A colourless, odourless liquid, with a sweet taste. Glycerol is seen in biological systems as an intermediate carbohydrate and lipid metabolism
Glycosidic Bond
a type of covalent bond that joins a carbohydrate molecule to another group
Hydrogen Bond
a weak bond between two molecules resulting from an electrostatic attraction between a proton in one molecule and an electronegative atom in the other
Hydrolysis
A chemical process of decomposition involving the splitting of a bond by the addition of water
Hydrophilic
a hydrophilic molecule is one whose interactions with water and other polar substances are more thermodynamically favourable than their interactions with oil or other hydrophobic solvents