Biological Molecules Flashcards
What elements do carbohydrates, proteins and lipids all have?
Carbon, hydrogen and oxygen (protein also has nitrogen)
What are carbohydrates made of?
starch (mainly in plants) / glycogen (mainly in plants), which are made up of long chains of simple sugars
What are simple sugars?
- e.g glucose
- the chemical name for a simple sugar is a monosaccharide
- when 2 simple sugars join together they form a disaccharide
- when lots of simple sugars join together they form a polysaccharide
- examples of polysaccharides are starch and cellulose and glycogen
What is the most common type of lipid found in the body?
Triacylglycerol
-made up of two different sub units called glycerin and fatty acids
Draw a diagram of a triaglycerol
Include: -glycerol, fatty acids
What are proteins?
- made up of long chains of amino acids
- there are 20 different types of amino acid and they can be joined together in different orders to make many different types of protein.
What is used to test for starch?
Iodine solution
What is a positive/negative result of starch by iodine solution
Positive result: blue/black colour
Negative result: orange/ yellow colour-doesn’t change
What is used to test for glucose?
Benedictus solution-must be heated to above 80°C
What is a positive/negative result of glucose by Benedicts solution?
Negative: pale blue
Small amount of glucose: green/yellow
Large amount of glucose: orange/red
Name things containing starch in the experiment
Bread, starch
Name the things (objects)used to test for glucose
Glucose (p), biscuits(p), egg white (n),rice(n)
Name the things (objects)used to test for fat
Crisps (p), lipid (p)
Name the things (objects)used to test for protein
Rice (n), tuna (p), albumen (p)
What is used to test for lipids
Ethanol
Briefly explain the practical for the test of starch
- place food on dropping tile
- drop iodine solution onto food
Briefly explain the practical for the test of glucose
- add the food to a test tube
- add the benedicts solution
- put in a water bath at 85°C and leave for 5-10 mins
Briefly explain the practical for the test of protein
- add the food to a test tube
- add a few drops of the biuret’s solution
Briefly explain the practical for the test of lipids
- add food to a test tube
- add a few drops of ethanol, put a bung on top and shake
- pour solution into a test tube containing water
What is a positive/ negative result for the test of protein with biurets solution
Negative: blue
Positive: purple/blue
What is a positive/negative result for the test of fats by ethanol
Positive: white emulsion forms
Negative: no white emulsion forms
What are enzymes?
- enzymes are biological catalysts
- they speed up chemical reactions in cells
- without enzymes, the reactions in cells would be too slow to support life
- some enzymes catalyse synthetic reactions (where large molecules are built-up from small molecules) e.g making glycogen from glucose (glucose synthase)
- other enzymes catalyse the breakdown reactions (splitting large molecules into smaller ones). E.g making glucose from glycogen (glycogen phosphorylase)
- enzymes are proteins: they are made up of long chains of amino acids which are folded into a specific shape
What is the lock and key hypothesis?
- it suggests that the substrate fits into an enzyme molecule like a key fits into a lock
- the active site of the enzyme fits onto ( complimentary shape) to the substrate
- the enzyme weakens the bonds in the substrate, causing it to split and form the products
- the products of the reaction are released at the end, leaving the enzyme free to be used again
What is a metabolic reaction?
The breaking down of a compound
What are the properties of enzymes?
- they work very rapidly
- only needed in small amounts
- they are specific: will only catalyse one reaction