Biological Molecules Flashcards
What is a monomer?
Small, single molecule, many of which can be joined to form polymers
What is a polymer?
Large molecule, made up of many similar molecules joined together
What is a condensation reaction?
It joins 2 molecules together and eliminates a water molecule which forms a chemical bond eg. Glycosidic bond
What is a hydrolysis reaction
It separates 2 molecules and requires addition of a water molecule which breaks a chemical bond
What is a monosaccharide?
The monomers from which larger carbohydrates are made
What is a disaccharide?
Formed by the condensation of 2 monosaccharides
What is a polysaccharides?
Formed by thecondensation of many monosaccharides
Structure of glucose
6 carbon atoms
Glucose + glucose =
Maltose
Glucose+ fructose =
Sucrose
Glucose+ galactose =
Lactose
What is an isomer?
Same molecular formula but differently arranged atoms
Difference in a-glucose and b-glucose
OH group is below c1 on a-glucose but above c1 in B- glucose
Example of polysaccharides
Starch, glycogen and cellulose
Function of glycogen
Energy store in animal cells
Structure of glycogen
Polysaccharide of a-glucose with c1-c4 and c1-c6 glycosidic bonds so branched branched I can be rapidly hydrolysed to release glucose for respiration to provide energy large polysaccharide molecule; can’t leave cell. Insoluble in water; water potential of cell not affected ie.no osmotic effect
Function of starch
Energy store in plant cells
Structure of starch
Polysaccharide of a-glucose. Mixture of amylose and amylopectin; amylose has c1-c4 glycosidic bonds so is unbranched, while amylopectin has cl-c4 and c1-c6 glycosidic bonds so is branched. Helical-compact for storage. Large polysaccharide- can’t leave cell. Insoluble in water - no osmotic effect
Function of cellulose
Provides strength and structural support to plant cell walls
Structure of cellulose
Every other beta glucose molecule is inverted in a long, straight, unbranched chain. Many hydrogen bonds link parallel strands (cross links) to form Micro fibrils (strong fibres). It bonds are strong in high numbers and provides strength and structural support to plant cell walls
Benedict’s test for reducing sugars
Add Benedict’s reagent (blue) to sample, heat in boiling water bath, positive= green, yellow , orange, red precipitate
Benedict’s test for non - reducing sugars
- Add a few drops of dilute hydrochloric acid (hydrolyse sugar into its constituent reducing sugars)
- Heat in a boiling water bath
- Neutralise with sodium carbonate
- Add Benedict’s reagent and heat again
- Non-reducing sugar present = green / yellow / orange / red precipitate
Determining glucose concentration
- Produce a dilution series of glucose solutions of known concentrations
- Perform a Benedict’s test on each sample
• Heat with Benedict’s solution
• Use same amount of solution for each test
• Use excess Benedict’s
• Remove precipitate by filtering - Using a colorimeter, measure the absorbance of each sample and plot a calibration curve
• Calibrate colorimeter using unreacted Benedict’s
• Use a red filter
• Less absorbance of filtrate = more sugar present (as removed precipitate)
• Plot absorbance against glucose concentration - Repeat with unknown sample (find absorbance) and use graph to determine glucose concentration
test for starch
Add iodine dissolved in potassium iodide to solution and shake stir. Blue-black colour = starch present
What are 2 groups of lipids
Triglycerides and phospholipids
What are triglycerides?
Triglycerides are formed by the condensation of 1 molecule of glycerol and 3 fatty acids
What forms an ester bond?
A condensation reaction between glycerol and a fatty acid (RCOOH)
Properties of triglycerides related to structure
• High ratio of C-H bonds to C atoms in hydrocarbon tail
so release more energy than the same mass of carbohydrates
• Insoluble in water (clump together as droplets)
so no effect on water potential of cell
What does a phospholipid consist of?
One fatty acids of a triglyceride is substituted by a phosphate -containing group. 1 fatty acid, glycerol, phosphate group
Phospholipid properties related to structure