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Biologia Molecular 2do Parcial > Biologia Molecular > Flashcards

Biologia Molecular Flashcards

Pasar 2do parcial

1
Q

Que es preanalitica

A

Desde la obtencion y preparacion preliminar y su transporte al laboratorio

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2
Q

Que es analitica

A

Aplicacion de procedimientos para la extraccion de acidos nucleicos en el laboratorio

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3
Q

Que es postanalitica

A

Validaciòn tecnica del informe analitico

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4
Q

Tipos de muestra celular

A

Somatica y germinativa

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5
Q

Tipos de genoma

A

Nuclear y mitocondrial

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6
Q

Tipos de estudio a realizar

A

Secuencia de genoma, genes y productos gericos

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7
Q

Tipos de muestra

A

Tejido, organo, fluido biologico

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8
Q

Que son las nucleasas

A

Enzima que catalisa los enlaces fosfodiester de la cadena polinucletidica

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9
Q

Donde realizan el corte las exonucleasas y endonucleasas

A

Al extremo de la cadena y dentro de la cadena

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10
Q

Donde cortan las tipo a y b

A

3’ y 5’

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11
Q

Funcion de las enzimas de restriccion

A

Reconocen una secuancia y realizan el corte en el lugar especifico

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12
Q

Aplicacion del uso de enzimas de restriccion

A

Tecnologia de DNA recombinante, clonacion celular o acelular, analisis del DNA, mapas fisicos de restriccion, polimorfismos, mutaciones

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13
Q

Que elementos requieren las nucleasas para llevar a cabo su funcion

A

Sitio, energia y un cofactor

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14
Q

Elementos de la nucleasa tipo l

A

endonucleasa y metilasa, ATP- Mg, No palindromico

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15
Q

Elementos de la nucleasa tipo ll

A

Endonucleasa, Mg, Palindromico

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16
Q

Elementos de la nucleasa tipo lll

A

Endonucleasa y metilasa, ATP- Mg, No palindromico

17
Q

Que es palindromo

A

Expresion que se lee igual de derecha a izquierda y viceversa

18
Q

Tipos de corte

A

Cohesivo o escalonado y romo

19
Q

Secuencia de reconocimiento de EcorI

A

GAATTC

20
Q

Que es la electroforesis

A

Separacion de fragmentos de DNA

21
Q

De donde provienen las enzimas de restriccion

A

De bacterias

22
Q

Tipos de gelels para electroforesis

A

Agarosa y Aclilamida

Biologia Molecular 2do Parcial (1 decks)

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