biolab Flashcards
cholesterol
where does cholesterol synthesis take place?
mainly in liver , and using simple molecules like acetate.
it also derived from dietary intake
what is cholesterol circulated with into circulation?
specific liboproteins
role of thyroid?
synthesis and degradation of cholesterol and triglycerides
what does bile acid do?
polar derivative of cholesterol that acts as detergents in the intestine, emulsifying dietary fats to make the more readily accessible to digestive lipases.
cholesterol uses-
important component of the cell membrane assisting in establishing proper membrane permeability and flexibility.
normal range of cholesterol in human-
150-250 mg/dl
primary factor for coronary heart disease-
cholesterol
elevated levels of serum cholesterol are associated with?
atherosclerosis, necrosis, diabetes mellitus.
low levels of cholesterol are associated with?
anaemia, hyperthyroidism, malabsorbtion
cholesterol esters + cholesterol esterase->
cholesterol + fatty acids
cholesterol + o2 + cholesterol oxidase ->
cholest-4-ene-3-one + h2o2
2h2o2 + phenol + 4-AAP + POD ->
quinononeimine dye(red) + h2o
what wavelength is absorbance of this quinoneimine dye measured at
505 (490-510) nm