BIOL1997 Flashcards
Module 3
What are examples of micronutrients?
- Vitamins
- Minerals
What are examples of macronutrients?
- Carbs
- Proteins
- Fats
What is the similarity and difference between protein and carbohydates?
o Similarity: Made out of monomers joined together to make a polymer
o Contrast: Carbohydrates have branches while proteins don’t
What does anabolic mean?
• Anabolic- building up
o Small molecules are assembled into large ones. Energy is required. Consumes ATP.
-Often intermediates
What does catabolic mean?
• Catabolic- breaking down
o Large molecules are broken down into small ones. Energy is released. Releases ATP.
-Often have intermediates
Where is glycogen, a carbohydrate, stored?
• Glycogen is stored in many cells, especially in the liver and skeletal muscle
What are proteins made of?
Amino acids
How many essential amino acids are there?
9
How many non-essential amino acids are there?
11
What is the general structure of amino acids?
A basic amino group, which at the pH of the cell, gains a proton and becomes –NH3+
An acidic carboxyl group, which at the pH of the cell, donates a proton and becomes –COO-
A variety of different side chain groups, abbreviated as R.
Do proteins need to get replaced?
Yes
What happens when proteins are damaged?
o Damaged proteins are broken down for recycling and new ones produced to replace them
How do proteins go through the cell from the ribosome?
o Protein has to stay unfolded to go through the cell so that it is able to go through the cell in a streamlined manner
o Hsp70 proteins are unraveled and linear so they can go to their needed point
o Hsp70 proteins go away and the protein can be correctly folded
o Happens near the nucleus
What is unsaturated fat?
o Unsaturated –when there is a double bond between the carbons
Double bond in unsaturated fatty acids is in a cis configuration, causing a ‘kink’ in the structure of the fatty acid chain
What is unsaturated fat?
o Saturated fatty acids- fatty acid chains that contain only carbon-carbon single bonds
Unsaturated fats is less destructive –> less capable of build fat up beneath arteries
What is fat made of?
Glycerol (one hydroxyl group per carbon) made of a carbon backbone, to which fatty acids (OH-C=O) are attached to make tryglicerides
How is fat transported through the blood?
Triglycerides coated in lipoproteins so they are no longer hydrophobic
Where is fat stored?
Adipose tissue, which can expand, grow and divide
What are lipids?
A diverse group of biomolecules including fats.
Defined as substances that can be extracted from biological materials using non-polar (hydrophobic) organic solvents
What are lipids made of?
Carbon, hydrogen and oxygen and have hydrophobic structures
Where are lipids stored?
Under epithelial of veins
How can ATP power cellular processes?
o Phosphate groups have high potential energy
o First phosphate is called alpha, second one beta and the last one gamma.
- Phosphates at the end of ATP have negative charges on them
- Last phosphate put on protein
- Negative charge of the gamma phosphate opens proteins up as it destroys negative-positive attractions of the proteins by binding to the proteins positive side and henceforth making the negatively charged parts repel from each other
- When an enzyme takes that away, the protein closes up again as negative charge is taken away
What is the general composition of the body?
o Liquid – 60% Infant- 70% Elderly person- 50~55% o Protein- 18% o Fat- 16% o Carbohydrates, Minerals, etc.-6%
What three macromolecules are convertible into each other?
Fats, proteins and carbohydrates
When you eat protein food, will you absorb the protein or will something else happen?
o Your body will break down into amino acids or dipeptides (rare anything bigger)
o Mainly absorbs amino acid
o Vilus have evolved to increase surface area so can get more amino acids into bloodstream
Where do animals have to get their essential amino acids from?
Plants- animals can’t make them themselves
Are carbs often converted into fat? Why?
Yes, because not a lot of space to store carbs
How are proteins, fats and carbohydrates interconverted?
Through glycolysis and the Kreb’s cycle
What are the 4 different types of tissues?
- Connective
- Muscle
- Nervous
- Epithilium
What are 3 examples of specialization and complex functions?
o Endocrine cells that can respond to homeostatic signals and release hormones Endocrine- no duct • Secretes hormones Exocrine - duct o Voluntary muscle o Fibroblasts that can repair wounds
Where exactly does mRNA get translated?
mRNA comes out of nuclear pores and gets translated in endoplasmic reticulum if the ribosomes are bound to the rough endoplasmic reticulum or free in the cytoplasm.
What is the difference between plant and animal cells?
Animal cell- Animal cells do not have chloroplasts Don’t have plastids Small vacuoles Don’t have a cell wall
Plant cell-
Plant cells have a cell wall
Have plastids
Large vacuoles
Describe the structure of the cell membrane
-Fluid mosaic structures that are composed of phospholipid bilayer containing proteins, glycoproteins and glycolipids
Describe the function of the cell membrane
- Separates cell from environment
- Regulates the passage of molecules in and out of cell to maintain cell integrity
- Selectively permeable
What is the function of the cytoplasm?
Acts as a medium where various organelles can remain suspended at their proper places
-Many chemical reactions take place in the cytoplasm
What is the function and structure of the nucleus?
It stores the information needed to control all cell activities.
The pores allow regulate the passage of substances (such as RNA and proteins) between the nucleus and the cytoplasm.
The nucleoplasm is liquid background in the nucleus where chromatin (made up of protein and nucleic acid) is found. The chromatin material also separates into chromosomes.
Nucleolus is a dense, granular region and contains a large amount of nucleic acid (mostly RNA) The nucleolus is responsible for the manufacture of ribosomes.
What is the structure of the plastid?
- Surrounded by a double membrane envelope and contains multiple copies of circular DNA, plus RNA and small ribosomes. Outer membrane high permeable to ions and smal molecules
- Inner membrane more selective contianing carrier proteins responsible for transporting molecules into and out of plastid
- Matrix enclosed within the inner membrane of a plastid is the stroma
What is the function of the plastid?
-Provide the cell with energy and sugars, and synthesises amino acids and most of a cell’s fatty acids.
What is the structure of the chloroplast?
Surrounded by double membrane which allows substances to pass between cytoplasm and chloroplast
Contains chlorophyll and thylakoids (parts of the membrane that form flattened disc-like sacs)
The layering of the membranes increases the surface area over which chlorophyll occurs, allowing a large amount of sunlight to be absorbed for the process of photosynthesis.
Photosynthetic pigments and enzymes are assembled on internal thykaloid membranes
What is the function of the chloroplast?
- Carry out photosynthesis
- Chloroplasts (a type of plastid) are responsible for the capture of energy from light during photosynthesis
What is the structure of the vacuole?
Consists of cell sap (watery solution) surrounded by a membrane called tonoplast.
Contains hydrolytic enzymes
What is the function of the vacuole?
- Place where nutrients, pigments or waste materials
- Helps cell keep its structure (maintains cell turgor pressure)
- Many of the functions that are carried out by animal cell lysosomes occur in one or more large membrane-bound vacuoles (serves as degradative compartment)
What is the structure and function of the cell wall?
Made of long strands of cellulose, lignin and pectin and support the structure of an organism, protect the cell and form a bridge to adjacent cells
- Rigidity and strength: allows plant to grow tall and hold out their leaves to capture light
- Physical barrier:protection
- Maintains shape and structure of cell
What is the structure of the cytoskeleton?
Network of tiny microbutules and microfilaments and intermediate filaments
- Microfilaments: composed of protein actin
- Microtubules: composed of tubulin
- Intermediate filaments: variable in structure and made of various proteins
What is the function of the cytoskeleton?
-Keeps organelles in place or move individual organelles or the whole cytoplasm from one place to another
-Facilitate maintenance and remodelling of cell shape
-Microfilaments contribute to structural organisation of cells and facilitate intracellular movement
Microtubules: dynamic scaffolding elements in eukaryotic cells
Intermediate filaments: strong, stable and resist stretching, and provide mechanical support
What is the structure of the microbodies?
- Spherical to sausaged shaped with homogenous, granular contents and sometimes crystalline inclusions.
- Surrounded by a sngle membrane, contain a variety of degradative enzymes (such as catalase)
What is the function of the microbodies?
-Main organelle involved in removal of compounds generated within cells
-Peroxisomes oxidise amino acids and uric acids
Glyoxysomes are involved in conversion of fatty acids to sugars
What is the structure of the mitochondria?
Outer membrane of double membrane gives mitochondria its shape and allows the passage of small substances into and out of mitochondria. Very permeable.
Inner membrane of double membrane is folded into cristae which increases the area for the attachment of groups of enzymes responsible for making energy for the cell. Highly impermeable
Central space in a mitochondrion is filled with fluid and is termed matrix which contains mitochondrial DNA and enzymes.
What is the function of the mitochondria?
- Produce energy by chemical respiration
- Released energy is stored in molecules of ATP which are used by cells to drive a vast range of chemical reactions.
What is the structure of the lysosomes?
Membrane bound organelles of animal cells involved in the degradation of many types of macromolecules.
Vesicle is filled with digestive enzymes for intercellular digestion
What is the function of the lysosomes?
Break down material ingested from outside the cell via endocytosis or phagocytosis
- Degrade worn out organelles via autophagy
- Allows macromolecules to be broken down to their constituent amino acids, sugars and nucleotides for reuse and secretion.
What is the structure of golgi bodies?
Consists of stacks of 4 to 10 disc-shaped cisternae and are surrounded by a cloud of small vesicles.
What is the function of golgi bodies?
-Secretory function. -Also
Processes, packages and sort cell products.
-Protein and glycoproteins enter each Golgi stack at the cis face and go through the body
-One mature products reach the trans face of the stack, they must be sorted and targeted to their correct destinations.
-Involved in the processing and packaging of glycoproteins and polysaccharides
What is the function of the transport vesicles?
Formed by Golgi apparatus move to and fuse with plasma membrane, and thereby release their contents to the outside of the cell in a process termed exocytosis and contents are taken up by endocytosis
-Small vesicles transport membrane and luminal contents between the ER, Golgi apparatus, endosomes, lysosomes, vacuoles and cell surface
What is the structure of the endoplasmic reticulum?
Network of membranous sacs (cisternae) that extends throughout the cytoplasm of eukaryotic cells
Smooth ER is the main site for lipid production, essential for membrane repair and manufacture.
Rough ER has ribosomes bound to its surface and folds/ processes protein products made by the cell.
What is the function of the endoplasmic reticulum?
Provides a connection of pathways between the nucleus and the cell’s environment allowing intracellular transport. However, it can also process cell products.
Involved in protein and lipid synthesis, and in detoxification of harmful compounds
What is the structure of ribosomes?
- Composed of two subunits, each of which is assembled in the nucleolus from RNA and protein molecules
- Subunits move through nuclear pores into the cytosol, where they associate with an mRNA molecule, forming a functional ribosome that facilitate protein synthesis.
What is the function of the ribosome?
Function is protein synthesis
What makes a prokaryotic cell and what are some examples of this?
o No membrane bound organelles
o Single cell
o Cell wall made of carbohydrates
o Don’t reproduce sexually to another cell
Use pilus to join to another cell and pull their cell membrane close, where they swap plasmids (genetic information)
o Bacteria- peptidoglycan (sugar and amino acid lattice) major constituent of cell wall
o Archaea- do not have peptidoglycan as major constituent of cell wall, often live in extreme conditions like anoxic mud, petroleum deposits and hot temperatures