Bioinformatics

Question 1

Difference between Sanger Sequencing Method and Next Generation Sequencing (NGS)

Answer 1

While the Sanger method only sequences a single DNA fragment at a time, NGS is massively parallel, sequencing millions of fragments simultaneously per run. See Wk2 slide 38.

Question 2

What is Transcriptomics

Answer 2

The study of the complete set of RNAs (transcriptome)
encoded by the genome of a specific cell or organism at
a specific time or under a specific set of conditions

Question 3

What is Proteomics

Answer 3

The study of the proteome. A proteome is a set of
proteins produced in an organism, system, or biological
context.
Proteomics are using techniques to quantify peptide
abundance, modification and interaction.

Question 4

What is Transcription

Answer 4

Process by which DNA is copied to
messenger RNA (mRNA) by RNA polymerases.

Question 5

Systems biology

Answer 5

It is a discipline for studying organisms as a network of interacting components

Question 6

Genomics

Answer 6

The entire sequence of an organism’s hereditary information, encoded in DNA is
known as the genome and their study is called genomics

Question 7

Model system

Answer 7

A simple system of networks which can be mapped to the problem under
consideration and is easily computationally modeled within available technologies.

Question 8

System

Answer 8

This term can be used to refer to a metabolic pathway, an organelle, a cell, a metabolic
system or an entire organism. The meaning of this term depends on the reference of the
problem.