Biofortification Flashcards

1
Q

How many people does hidden hunger affect?

A

Estimated 2 billion

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2
Q

Main limiting nutrients in peoples diets?

A

Iodine, Vit A, Iron

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3
Q

why do we need iodine and where can we find it, both naturally and unnaturaly

A

For thyroid hormone synthesis. In seaweed naturally. Can get through salt or indirectly through animal food.

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4
Q

Three enzymes involved in golden rice?

A
  1. Phytoene synthase
  2. Phytoene Desaturase
  3. Carotene desaturase
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5
Q

Agrobacterium based vectors require what?

A

Right and left borders, promotor, gene, nos terminator, selective marker

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6
Q

What is bioremediation and how is it useful?

A

Can concentrate toxic chemicals in high concentrations to clean polluted land

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7
Q

How many plants are hyperaccumulators?

A

721 (can be used in mining)

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8
Q

Two ways to take up iron in plants

A

Reduce Fe3+ to Fe2+ (more soluble)

Chelators (siderophores) bind with iron and take up into roots.

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9
Q

Biggest consumers of rice?

A

China and India

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10
Q

Two ways to increase iron uptake in biotech

A

Increase NAS enzyme level that is involved in creating DMA (a chelator)
ferretin protein to increase iron storage with pr2 promotor.

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11
Q

Approach of the yeast model

A

To change metal composition, we can introduce mutant that can take up ions.

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12
Q

What does Brussin blue stain shoe in ths experiment?

A

Visualise iron concentration

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13
Q

What is the specific aim of this biotechnology

A

To increase iron concentration in the seed only.

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14
Q

Define an alkaloid

A

Chemical containing N stimulants or narcotics

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15
Q

What is a terpenoid?

A

Organic compound from isoprene subunits

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16
Q

What are phenolics?

A

Tannins

17
Q

Where do you find opium and what are its effects?

A

dried latex from seed capsule of poppy seed - pain relief but also highly addictive

18
Q

What is the point of CSIRO poppy?

A

Accumulates thebaine and and oripavine (pain relief) but not codeine or morphine (addictive)

19
Q

Top plant supplements?

A

Echinacae, garlic, ginseng

20
Q

Process of bioprospecting?

A

Search for species from which medicinal drugs and other commercially valuable compounds can be obtained. Chemical extraction and assays.