Biocides

Question 1

Biocides mode of action

Answer 1

Multiple target sites; non-specific at high concentrations
increases permeability of outer membrane
Increases permeability, membrane potential and enzymic activity of cytoplasmic membrane.
Causes general coagulation of cytoplasmic constituents

Question 2

Triclosan

Answer 2

Blocks action of bacterial enoyl-acyl carrier protein reductase
Widespread use
tentative evidence that resistance may be transferable

Question 3

Summary:

Answer 3

Biocide use is widespread
To date, little evidence of development of resistance during appropriate use of biocides.
Cross-resistance with antibiotics? Possible to demonstrate in the laboratory but, in reality, little compelling evidence thus far
Effect of residual levels not fully investigated