Biochemistry Practice Exam

Question 1

What is the definition of a product?

Answer 1

Substance formed from the substrate at the end of a reaction with an enzyme

Question 2

What is the definition of an active site?

Answer 2

Regions on surfaces of the enzymes that fit the substrate

Question 3

What is the definition of an enzyme?

Answer 3

Substances that bring about a chemical reaction without being changed itself.

Question 4

What is the definition of a catalyst?

Answer 4

Proteins that speed up chemical reactions

Question 5

What is the definition of a substrate?

Answer 5

Substance that an enzyme acts upon

Question 6

What is the definition of activation energy?

Answer 6

Amount of energy required for a chemical reaction to occur.

Question 7

What are 3 factors that affect enzyme activity?

Answer 7

Substrate concentration, pH, temperature

Question 8

Define V0

Answer 8

velocity (rate) of reaction (initial rate of reaction)

Question 9

Define Vmax

Answer 9

The maximum rate of reaction achieved (all enzymes become saturated with substrate

Question 10

Define Km

Answer 10

Michaelis constant (substrate concentration at which half of the Vmax is achieved)

Question 11

In a competitive Lineweaver-Burk plot, what is significant about the Vmax and Km values?

Answer 11

Competitive
Vmax = same
Km = different

Question 12

In a uncompetitive Lineweaver-Burk plot, what is significant about the Vmax and Km values?

Answer 12

Uncompetitve
Vmax = different
Km = different

Question 13

In a non-competitive (mixed) Lineweaver-Burk plot, what is significant about the Vmax and Km values?

Answer 13

Non-competitive
Vmax = different
Km = same