Biochemistry and Lab Techniques Flashcards

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1
Q

fatty acids

A

long carbon chains with a carboxylic acid end

  • hormones/intracelular messengers
  • components of cell membrane
  • act as fuel for body
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2
Q

lipolysis

A

tricylglycerols hydrolyzed to form glycerol and the corresponding fatty acids
reverse of esterification

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3
Q

saponification

A

triacylglycerols cleaved by addition of NaOH, key reaction in production of soap

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4
Q

since the hydrophobic carbon chain predominated, fatty acids are ____

A

nonpolar

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5
Q

fatty acids enter into the ____ ____ 2 carbons at a time

A

Kreb’s cycle

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6
Q

a peptide bond creates a functional group known as an ___

A

amide

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7
Q

the R group is called the ____ of the aino acid

A

side chain

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8
Q

____ alpha amino acids

A

20

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9
Q

___ essential amino acids

A

10

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10
Q

polar side groups are ____ and nonpolar side groups are ____

A

polar are hydrophlic, nonpolar are hydrophobic

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11
Q

isoelectric point, pI

A

pH where the population has no net charge and the max number of species are zwitterons, pH at first equivalence point

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12
Q

all acidic and basic R groups are also ____

A

polar

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13
Q

only the three ____ amino acids have an isoelectric point above a pH of 7

A

basic, all other aa’s have pI below 7

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14
Q

hexoses

A

6 carbon carbohydrates, glucose and fructose

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15
Q

glucose is an ____ and fructose is a ____

A

aldehyde, ketone

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16
Q

aldoses and ketoses

A

aldose: polyhydroxyaldehydes like glucose
ketoses: polyhydroxyketones like fructose

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17
Q

aldohexose

A

glucose

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18
Q

if hydroxyl group on highest numbered chiral carbon point to the right…

A

carb is labeled D

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19
Q

anomeric carbon of ring structure of hexoses

A

only carbon attached to two oxygens, alcohol group may point up or down-alpha or beta anomer

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20
Q

5 membered ring

A

furanose

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21
Q

six membered ring

A

pyranose

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22
Q

names of reducing sugars end is ____, names of nonreducing sugars end in ___

A

-ose, -oside

23
Q

sucrose

A

glucose + fructose

1,1’ glycosidic linkage (1,2’ linkage)

24
Q

maltose

A

glucose + glucose

alpha-1,4’l glucosidic linkage

25
Q

lactose

A

galactose + glucose

beta-1,4’ galactosidic linkage

26
Q

cellulose

A

chain of glucose

beta-1,4’ glucosidic linkage

27
Q

amylose (starch)

A

chain of glucose

alpha-1,4’ glucosidic inkage

28
Q

amylopectin

A

branched chain of glucose
alpha-1,4’ glucosidic linkage
alpha-1,6’ linkages forming branches

29
Q

glycogen

A

branched chain of glucose
alpha-1,4’ glucosidic linkage
alpha-1,6’ linkages forming branches

30
Q

animals cannot break down ____ linkages

A

beta-1,4’ linkages in cellulose

31
Q

3 types of spectroscopy

A
nuclear magnetic resonance (nmr)
infrared spectroscopy (IR)
ultraviolet spectroscopy (UV)
32
Q

nmr is concerned with ____

A

hydrogens

33
Q

nmr: each peak represents ……

A

chemically equivalent hydrogens, have same chemical shift

34
Q

nmr: splitting of peaks is created by….

A

neighboring hydrogens

35
Q

chemical shift

A

difference between the resonance frequency of the chemically shifted hydrogens and the resonance frequency of hydrogens on a reference compound

36
Q

the are under a nmr peak is ….

A

proportional to the number of hydrogens represented by that peak

37
Q

integral trace

A

line drawn about the peaks that rises each time it goes over a peak, rise in proportion to number of hydrogens in peak below it

38
Q

nmr splitting results from…

A

neighboring hydrogens that are not chemically equivalent

39
Q

number of peaks due to splitting for a group of chemically equivalent hydrogens is given by…

A

n+1, n is number of neighboring hydrogens that are not chemically equiv.

40
Q

nmr: electron withdrawing groups and donating groups

A

withdrawing: less shielding, downfield to left
donating: more shielding, upfield to right

41
Q

nmr: aldehyde protons have very distinct shift at ___

A

9.5 ppm

42
Q

IR spectroscopy

A

infrared spectrometer slowly changes the frequency of infrared light shining upon a compound and records the frequencies of absorption

43
Q

IR: fingerprint region

A

600 to 1400 cm^-1 region, complex vibrations that distinguish one compound from a similar compound

44
Q

UV spectrosocopy detects…

A

conjugated double bonds (double bonds separated by one single bond) by comparing intensities of two means of light from the same monochromatic light source

45
Q

UV: for each additional conjugated double bond and alkyl group…

A

30-40 nm increase for each conjugated double bond,

5 nm increase for each additional alkyl group

46
Q

UV: isolated double bonds….

A

do not increase the absorption wavelength

47
Q

beta-carotene

A

precursor of vit. A, 11 conjugated double bonds-absorbance moves into visible spectrum, absorb blue-green light giving them red/orange complementary color

48
Q

mass spectrometry

A

gives the molecular weight and sometimes molecular formula

49
Q

mass spec: parent peak

A

peak made by molecules that did not fragment

50
Q

chromatography

A

the resolution/separation of a mixture by passing it over or through a matrix that absorbs different compound with different affinities

51
Q

distillation

A

separation based upon vapor pressure

52
Q

pure substances form…

A

crystals

53
Q

extraction based upon…

A

solubility due to similar polarities, start with organic mixture on top of aqueous solution

54
Q

3 steps of extraction

A

add strong acid: pronates amines/bases removed
add weak base: deprotonates strong acids, dissolve, and drained off
add strong base: reacts with rest of acids, drained off