Biochemistry and Lab Techniques Flashcards

(54 cards)

1
Q

fatty acids

A

long carbon chains with a carboxylic acid end

  • hormones/intracelular messengers
  • components of cell membrane
  • act as fuel for body
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

lipolysis

A

tricylglycerols hydrolyzed to form glycerol and the corresponding fatty acids
reverse of esterification

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

saponification

A

triacylglycerols cleaved by addition of NaOH, key reaction in production of soap

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

since the hydrophobic carbon chain predominated, fatty acids are ____

A

nonpolar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

fatty acids enter into the ____ ____ 2 carbons at a time

A

Kreb’s cycle

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

a peptide bond creates a functional group known as an ___

A

amide

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

the R group is called the ____ of the aino acid

A

side chain

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

____ alpha amino acids

A

20

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

___ essential amino acids

A

10

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

polar side groups are ____ and nonpolar side groups are ____

A

polar are hydrophlic, nonpolar are hydrophobic

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

isoelectric point, pI

A

pH where the population has no net charge and the max number of species are zwitterons, pH at first equivalence point

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

all acidic and basic R groups are also ____

A

polar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

only the three ____ amino acids have an isoelectric point above a pH of 7

A

basic, all other aa’s have pI below 7

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

hexoses

A

6 carbon carbohydrates, glucose and fructose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

glucose is an ____ and fructose is a ____

A

aldehyde, ketone

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

aldoses and ketoses

A

aldose: polyhydroxyaldehydes like glucose
ketoses: polyhydroxyketones like fructose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

aldohexose

A

glucose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

if hydroxyl group on highest numbered chiral carbon point to the right…

A

carb is labeled D

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

anomeric carbon of ring structure of hexoses

A

only carbon attached to two oxygens, alcohol group may point up or down-alpha or beta anomer

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

5 membered ring

A

furanose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

six membered ring

A

pyranose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

names of reducing sugars end is ____, names of nonreducing sugars end in ___

23
Q

sucrose

A

glucose + fructose

1,1’ glycosidic linkage (1,2’ linkage)

24
Q

maltose

A

glucose + glucose

alpha-1,4’l glucosidic linkage

25
lactose
galactose + glucose | beta-1,4' galactosidic linkage
26
cellulose
chain of glucose | beta-1,4' glucosidic linkage
27
amylose (starch)
chain of glucose | alpha-1,4' glucosidic inkage
28
amylopectin
branched chain of glucose alpha-1,4' glucosidic linkage alpha-1,6' linkages forming branches
29
glycogen
branched chain of glucose alpha-1,4' glucosidic linkage alpha-1,6' linkages forming branches
30
animals cannot break down ____ linkages
beta-1,4' linkages in cellulose
31
3 types of spectroscopy
``` nuclear magnetic resonance (nmr) infrared spectroscopy (IR) ultraviolet spectroscopy (UV) ```
32
nmr is concerned with ____
hydrogens
33
nmr: each peak represents ......
chemically equivalent hydrogens, have same chemical shift
34
nmr: splitting of peaks is created by....
neighboring hydrogens
35
chemical shift
difference between the resonance frequency of the chemically shifted hydrogens and the resonance frequency of hydrogens on a reference compound
36
the are under a nmr peak is ....
proportional to the number of hydrogens represented by that peak
37
integral trace
line drawn about the peaks that rises each time it goes over a peak, rise in proportion to number of hydrogens in peak below it
38
nmr splitting results from...
neighboring hydrogens that are not chemically equivalent
39
number of peaks due to splitting for a group of chemically equivalent hydrogens is given by...
n+1, n is number of neighboring hydrogens that are not chemically equiv.
40
nmr: electron withdrawing groups and donating groups
withdrawing: less shielding, downfield to left donating: more shielding, upfield to right
41
nmr: aldehyde protons have very distinct shift at ___
9.5 ppm
42
IR spectroscopy
infrared spectrometer slowly changes the frequency of infrared light shining upon a compound and records the frequencies of absorption
43
IR: fingerprint region
600 to 1400 cm^-1 region, complex vibrations that distinguish one compound from a similar compound
44
UV spectrosocopy detects...
conjugated double bonds (double bonds separated by one single bond) by comparing intensities of two means of light from the same monochromatic light source
45
UV: for each additional conjugated double bond and alkyl group...
30-40 nm increase for each conjugated double bond, | 5 nm increase for each additional alkyl group
46
UV: isolated double bonds....
do not increase the absorption wavelength
47
beta-carotene
precursor of vit. A, 11 conjugated double bonds-absorbance moves into visible spectrum, absorb blue-green light giving them red/orange complementary color
48
mass spectrometry
gives the molecular weight and sometimes molecular formula
49
mass spec: parent peak
peak made by molecules that did not fragment
50
chromatography
the resolution/separation of a mixture by passing it over or through a matrix that absorbs different compound with different affinities
51
distillation
separation based upon vapor pressure
52
pure substances form...
crystals
53
extraction based upon...
solubility due to similar polarities, start with organic mixture on top of aqueous solution
54
3 steps of extraction
add strong acid: pronates amines/bases removed add weak base: deprotonates strong acids, dissolve, and drained off add strong base: reacts with rest of acids, drained off