Biochemistry Flashcards
cell respiration
metabolic reactions and processes which take place in cells of organisms to convert biochemical energy (found in nutrients) into ATP
controlled release of energy from organic compounds to produce ATP
cells which perform cell respiration
all living cells
source of energy for cell respiration (in animals)
food
source of energy for cell respiration (in plants)
photosynthesis
purpose of cell respiration
conversion of high-energy compounds (such as glucose) into low-energy molecules (such as ATP)
adenosine triphosphate
ATP
nucleid acid which functions as source of energy for cell processes
informally known as “currency of the cell”
most often produced by mitochondria during cell respiration
source of energy for cell processes
ATP
reason that carbohydrates and lipids cannot be used as source of energy for cell processes
energy from carbohydrates and lipids is too high for usage in cell processes (must be broken down into lower-energy molecules)
method of dissipation of energy from ATP
heat
efficiency of cell respiration
inefficient
efficiency of cellular use of ATP
inefficient
oxidation reaction
chemical reaction which involves the loss of electrons from an atom
often includes gain of oxygen atom or loss of hydrogen atom
causes atom to become more positive

reduction reaction
chemical reaction which involves the gain of electrons from an atom
often includes loss of oxygen atom or gain of hydrogen atom
causes atom to become more negative

redox reaction
reduction-oxidation reaction
chemical reaction wherein oxidation reaction and reduction reaction occur simultaneously

reason that oxidation reactions and reduction reactions are dependent upon each other
oxidation involves loss of electrons in 1 atom
reduction involves gain of electrons in another atom
effect of electron transfer in redox reactions
release of energy stored in organic molecules (used to make ATP)
catabolic pathway
series of reactions that bring about net release of energy by breaking down complex organic molecules

anabolic pathway
series of reactions that require energy to compose complex organic molecules

endergonic reaction
chemical reaction with a net loss of energy
exergonic reaction
chemical reaction with a net gain of energy
phosphorylation
attachment of phosphate group
endergonic reaction
destabilizes molecules (increases reactivity)
dephosphorylation
detatchment of phosphate group
exergonic reaction
stabilizes molecules (decreases reactivity)
significance of phosphorylation of ADP
converts ADP (which is stable) into ATP (which is reactive)
nicotinamide adenine dinucleotide
NAD
main electron carrier in cell respiration
transports electrons between reactions in redox reactions
NAD+
oxidized form of nicotinamide adenine dinucleotide
NADH
reduced form of nicotinamide adenine dinucleotide
aerobic cell respiration
metabolic reactions and processes which require oxygen to convert biochemical energy (found in nutrients) into ATP
primary reaction site of aerobic cell respiration
mitochondrion
process of aerobic cell respiration
glycolysis
pyruvate oxidation
Krebs cycle
oxidative phosphorylation

chemical equation of aerobic cell respiration

glycolysis
catabolic pathway that converts 1 glucose molecule into 2 smaller pyruvate molecules
occurs in cytoplasm
anaerobic process
chemical formula of glucose
C6H12O6
activation energy of glycolysis
2 ATP
total ATP yield of glycolysis
4 ATP
net yield from glycolysis
2 pyruvate molecules
2 ATP
2 NADH
2 hydrogen ions
chemical formula of pyruvate
CH3COCOO1-
pyruvate oxidation
pyruvate decarboxylation
link reaction
catabolic pathway that converts pyruvate into acetyl coenzyme A
occurs in mitochondrion
transport method of pyruvate from cytoplasm to mitochondion (and why)
active transport (because pyruvate is charged)
process of pyruvate oxidation
decarboxylation of pyruvate
connection between acetyl group and coenzyme A

net yield of decarboxylation of pyruvate
1 acetyl group
1 carbon dioxide molecule
chemical formula of acetyl group
CH3CO
chemical formula of carbon dioxide
CO2
enzyme required for pyruvate oxidation to occur
coenzyme A
coenzyme A
CoA
enzyme required for pyruvate oxidation to occur
joins with acetyl group to form acetyl coenzyme A
acetyl coenzyme A
acetyl-CoA
compound formed by pyruvate oxidation
composed of coenzyme A and acetyl group
net yield of pyruvate oxidation
1 acetyl coenzyme A molecule
1 NADH
Krebs cycle
citric acid cycle
metabolic pathway which releases stored energy through oxidation of acetyl coenzyme A
occurs in mitochondrion
process of Krebs cycle
combination of acetyl coenzyme A and oxaloacetate (formation of citric acid)
decarboxylation (release of 2 carbon dioxide molecules)
reduction of NAD+ into NADH
combination of ADP and Pi (formation of ATP)
reduction of FAD into FADH2
formation of oxaloacetate

net yield of Krebs cycle
4 carbon dioxide molecules
2 ATP
6 NADH
2 FADH2
oxidative phosphorylation
metabolic pathway that converts nutrients into ATP
occurs in mitochondrion
efficiency of oxidative phosphorylation (in comparison to fermentation)
highly efficient
components of oxidative phosphorylation
electron transport chain
chemiosmosis

electron transport chain
ETC
series of complexes that transfer electrons from electron donors to electron acceptors (via redox reactions)

functions of electron transport chain in cell respiration
transfer electrons across inner-mitochondrial membrane from NADH to oxygen
use proteins to transfer hydrogen ions across membrane of mitochondrial matrix
chemical equation of oxidation of NADH in oxidative phosphorylation

chemical equation of reduction of oxygen in oxidative phosphorylation

final electron acceptor in electron transport chain
oxygen
chemiosmosis
movement of ions across a semipermeable membrane (down concentration gradient)

ATP synthase
enzyme which creates ATP by combining ATP and Pi

net yield of oxidative phosphorylation
approximately 28 ATP
1 water molecule
anaerobic cell respiration
fermentation
metabolic reactions and processes which do not require oxygen to convert biochemical energy (found in nutrients) into ATP
efficiency of anaerobic cell respiration
inefficient
3 conditions under which anaerobic cell respiration occurs
need for short (but rapid) burst of ATP production
depletion of oxygen supplies in respiring cells
placement in environment which lacks oxygen
anaerobic cell respiration which occurs in animal cells
lactic acid fermentation
anaerobic cell respiration which occurs in plants and yeast cells
alcohol fermentation
lactic acid fermentation
lactate fermentation
catabolic pathway which converts 1 glucose molecule into 2 lactate molecules
process of lactic acid fermentation

net yield of lactic acid fermentation
2 lactate molecules
2 ATP
alcohol fermentation
catabolic pathway which converts 1 glucose molecule into 2 ethanol molecules
process of alcohol fermentation

net yield of alcohol fermentation
2 ethanol molecules
1 carbon dioxide molecule
2 ATP
anaerobic organism
anaerobe
organism which does not require oxygen for growth
obligate anaerobes
organism which does not require oxygen for growth
cannot survive in presence of oxygen
facultative anaerobe
organism which does not require oxygen for growth
able to survive in presence of oxygen
photosynthesis
conversion of light energy into chemical energy
occurs only in plant and algae organisms
primary reaction site of photosynthesis
chloroplast
chemical equation of photosynthesis
chlorophyll
primary photosynthetic pigment in plant cells and algae cells
located within chloroplast
chlorophyll a
photosynthetic pigment which converts solar energy into chemical energy
participates directly in light-dependent reactions
chlorophyll b
photosynthetic pigment that transfers energy to chlorophyll a
participates indirectly in light-dependent reactions
carotenoid
photosynthetic pigment which broadens spectrum of colors which can be absorbed in photosynthesis
participates indirectly in light-dependent reactions
2 main processes of photosynthesis
light-dependent reactions
light-independent reactions
light-dependent reactions
chemical reactions of photosynthesis which use light energy to produce ATP and NADPH
photosystem
integral proteins found within thylakoid membranes
large protein complexes and pigments which are optimized to harvest light

photosystems in photosynthesis (in order of reactions)
photosystem II
photosystem I
pair of chlorophyll molecules found in photosystem II
P680
pair of chlorophyll molecules found in photosystem I
P700
input of photosystem II
water molecules
light
functions of photosystem II
absorb light
split water molecules
output of photosystem II
plastoquinone
input of cytochrome complex
plastoquinone
cytochrome complex
cytochrome b6f complex
enzyme which catalyzes transfer of electrons from plastoquinone to plastocyanin
output of cytochrome complex
plastocyanin
ATP
input of photosystem I
plastocyanin
function of photosystem I
transfer electrons from plastocyanin to ferredoxin
output of photosystem I
ferredoxin
input of NADP+ reductase
electrons from ferredoxin
NADP+
2 hydrogen ions
function of NADP+ reductase
reduce NADP+ to NADPH
output of NADP+ reductase
NADPH
1 hydrogen ion
(Calvin cycle)
light-independent reactions
Calvin cycle

chemical reactions of photosynthesis which use carbon dioxide molecules into sugar
(Calvin cycle)
3 phases of Calvin cycle
carbon fixation
reduction
RuBP regeneration
ribulose biphosphate
RuBP
5-carbon sugar used in process of photosynthesis
ribulose biphosphate carboxylase
RuBisCO
enzyme which facilitates carboxylation of RuBP
used in carbon fixation
glyceraldehyde-3-phosphate
G3P
triose phosphate
TP
3-carbon sugar used in process of photosynthesis
used to create glucose molecules in Calvin cycle
3-phosphoglycerate
3PG
3-carbon sugar used in process of photosynthesis
used as intermediate in Calvin cycle
conjugate acid of G3P
1,3-biphosphoglycerate
1,3BPG
3-carbon sugar used in process of photosynthesis
used as intermediate in glycolysis and Calvin cycle
(Calvin cycle)
carbon fixation
1st phase of Calvin cycle
carboxylation of RuBP
(Calvin cycle)
input of carbon fixation
3 RuBP
3 carbon dioxide molecules
(Calvin cycle)
steps of carbon fixation
RuBP is carboxylated using RuBisCO
unstable 6-carbon intermediate is formed
short-lived intermediate splits into 3PG

(Calvin cycle)
output of carbon fixation
6 3PG
(Calvin cycle)
reduction
2nd phase of Calvin cycle
conversion of 3PG into G3P
(Calvin cycle)
input of reduction
6 3PG
6 ATP
6 NADPH
(Calvin cycle)
steps of reduction
3PG gains another phosphate group (by converting ATP to ADP)
1,3BPG is formed
1,3BPG is reduced (by converting NADPH into NADP+ and Pi)
G3P is formed
G3P molecule is released to form glucose (or other sugar molecule)

(Calvin cycle)
output of reduction
6 G3P
6 ADP
6 NADP+
6 Pi
(Calvin cycle)
RuBP regeneration
third phase of Calvin cycle
conversion of G3P into RuBP
(Calvin cycle)
input of RuBP regeneration
5 G3P
3 ATP
(Calvin cycle)
steps of RuBP regeneration
G3P undergoes structural changes
gains another phosphate group (by converting ATP to ADP)
RuBP is formed

(Calvin cycle)
output of RuBP regeneration
3 RuBP
3 ADP
photorespiration
metabolic process wherein oxygen molecules replace carbon dioxide molecules in RuBisCO
wasteful of carbon dioxide molecules produced by photosynthesis
limiting factors of photosynthesis
light intensity
temperature
carbon dioxide concentration