BIOCHEMICAL TESTS FOR GRAM-NEGATIVE BACILLI

Question 1

KIA
LIA
TSI

Answer 1

Kligler’s Iron Agar
lysine Iron Agar
Triple Sugar Iron

Question 2

Are the test used for the identification of bacteria species.

Answer 2

Biochemical

Question 3

Is used to determine whether or not a bacteria can utilize a certain carbohydrate.

Answer 3

Fermentation

Question 4

These are tests designed to demonstrate the physiological and chemical characteristics of microorganisms

Answer 4

Biochemical test

Question 5

demonstrate the ________________ of a bacterial cell

Answer 5

Enzyme system

Question 6

anaerobic breakdown of carbohydrates resulting often in the production of large amounts of organic acids

Answer 6

Carbohydrate fermentation

Question 7

Common carbohydrates used in the media:

Answer 7

Glucose- monosaccharide
Lactose- disaccharide
sucrose- disaccharide

Question 8

Enzyme in lactose fermentation

Answer 8

beta- galactoside permease
beta- galactosidase

Question 9

posses both enzyme

Answer 9

Lactose fermenter {LF}

Question 10

Do not posses both enzymes

Answer 10

Non-lactose fermenter {NLF}

Question 11

posses only beta- galactosidase

Answer 11

Late lactose fermenter {LLF}