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Clinical Bacteriology (Lab) > Biochemical ID of Gram (-) Bacteria > Flashcards

Biochemical ID of Gram (-) Bacteria Flashcards

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1
Q

What family is also referred to as “enterics”

A

Family Enterobacteriaceae

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2
Q

What family is gram-negative bacilli and coccobacilli (rods), non- spore forming, facultative anaerobic composition??

A

Family Enterobacteriaceae

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3
Q

Family Enterobacteriaceae do not produce _____ except Plesiomonas

A

cytochrome C oxidase

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4
Q

Family Enterobacteriaceae are motile at body temperatures except what bacteria?

A

Klebsiella, Shigella and Yersinia

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5
Q

In the specimen collection of the laboratory diagnosis, fecal specimens or stool samples are collected, usually
within first ____ days of illness and ____ the
administration of antibiotic.

A

four (4); before

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6
Q

In the specimen collection of the laboratory diagnosis, Specimen should be processed within what time
of collection?; if not, place it to appropriate transport
media and kept in what temperature?

A

1-2 hours; 4 ̊C

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7
Q

In the gram stain of the laboratory diagnosis, Direct smear examination of stool samples is not particularly
helpful in identifying enteric pathogens but may reveal the presence of _____

A

inflammatory cells

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8
Q

Cultures grow at an optimal temperature of ______

A

35°C to 37°C

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9
Q

Cultures grow at an low temperatures of ______°C, example of a bacteria is _______

A

1-5;Serratia and Yersinia

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10
Q

Cultures grow at an low temperatures of ______°C, example of a bacteria is _______

A

45-50°C; E. coli

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11
Q

To ensure isolation of both opportunistic and
fastidious pathogens, laboratories must provide
appropriate transport media, such as ________

A

Cary-Blair, Amies, or Stuart media

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12
Q

All Enterobacteriaceae produce similar growth on ___ agars.

A

blood and chocolate

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13
Q

What bacteria may be mucoid because of their polysaccharide capsule

A

Klebsiella or Enterobacter

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14
Q

What bacteria “swarm” on blood and chocolate agars.

A

P. mirabilis, P. penneri, and P. vulgaris

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15
Q

Broth cultures of Y. pestis exhibit what characteristic?

A

“stalactite pattern”

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16
Q

What bacteria produces bull’s-eye colonies?

A

Y. enterocolitica

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17
Q

Agar that is best used to characterize gram negative rods

A

MacConkey AGAR

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18
Q

What agar is selective for gram-negative enteric bacilli

A

MacConkey AGAR

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19
Q

Lactose fermenter have what color of colonies?

A

dark pink colonies

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20
Q

Time of incubation of a Rapid Lactose Fermenter

A

18 to 24 hours

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21
Q

Time of incubation of a Late Lactose Fermenter

A

48 to 72 hours

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22
Q

Identify the bacteria if it is a Rapid or Late Lactose Fermenter: Escherichia

A

Rapid Lactose Fermenter

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23
Q

Identify the bacteria if it is a Rapid or Late Lactose Fermenter: Enterobacter

A

Rapid Lactose Fermenter

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24
Q

Identify the bacteria if it is a Rapid or Late Lactose Fermenter: Klebsiella

A

Rapid Lactose Fermenter

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25
Identify the bacteria if it is a Rapid or Late Lactose Fermenter: Hafnia
Late Lactose Fermenter
26
Identify the bacteria if it is a Rapid or Late Lactose Fermenter: Citrobacter
Late Lactose Fermenter
27
Identify the bacteria if it is a Rapid or Late Lactose Fermenter: Serratia
Late Lactose Fermenter
28
Identify the bacteria if it is a Rapid or Late Lactose Fermenter: Salmonella arizonae
Late Lactose Fermenter
29
Identify the bacteria if it is a Rapid or Late Lactose Fermenter: Shigella sonnei
Late Lactose Fermenter
30
Identify the bacteria if it is a Rapid or Late Lactose Fermenter: Yersinia enterocolitica
Late Lactose Fermenter
31
Non-lactose fermenter have what color of colonies?
clear
32
In the biochemical tests on carbohydrate utilization, what bacterium serve as transport enzyme to facilitate the lactose to enter the plasma membrane of the bacteria?
B-galactoside permease or lactose permease
33
In the biochemical tests on carbohydrate utilization, what is the enzyme that hydrolyzes lactose into glucose and galactose
B-galactosidase
34
What fermenter possess both B-galactoside permease and B- galactosidase
LFs
35
What fermenter do not possess either enzyme
NLFS
36
What fermenter lack ẞ-galactoside permease but possess ẞ- galactosidase
LLFS
37
OF Basal Medium produces what color on pH indicator?
brom thymol blue
38
OF Basal Medium produces what color on uninoculated medium?
green
39
OF Basal Medium produces what color on alkaline environment?
blue
40
These reaction are produced by what element? "Acid produced on both tubes"
oxidizer and fermenter
41
These reaction are produced by what element? "Acid only in closed tube"
fermenter and possible obligate anaerobe
42
These reaction are produced by what element? "Acid in open tube:"
Oxidizer
43
What test is used to determine glucose and lactose or sucrose utilization and H2S production, gas production
TRIPLE SUGAR IRON (TSI)
44
What is the composition of TSI?
10 parts lactose, 10 parts sucrose, 1 part glucose and peptone
45
What is the pH indicator of TSI?
Phenol Red - Yellow
46
What is the ideal pH for the TSI to be called acidic?
pH 6.8
47
What is the H2S indicator of TSI?
Ferrous sulfate and Sodium thiosulfate
48
Reactions should be read within an ______ (time) incubation period; otherwise, erroneous results are possible.
18- to 24-hour
49
What is the fermenters present on A/A reaction on TSI agar
Lactose fermenter Sucrose fermenter Glucose fermenter
50
What is the fermenters present on K/A reaction on TSI agar?
Glucose fermenter
51
What is the fermenters present on K/K reaction on TSI agar?
No fermentation - not a member of Enterobacteriaceae
52
What are the H2S-producing bacteria in Triple Sugar Iron?
Salmonella Proteus Arizona Citrobacter Edwardsiella
53
What test determine the ability of organism to produce B- galactosidase that hydrolyzes ONPG, a colorless compound, into galactose and o-nitrophenol, a yellow compound (lactose fermenter)
ORTHO-NITROPHENYL-B-D-GALACTOPYRANOSIDE TEST (ONPG TEST)
54
ONPG remains what color if the organism is an NLF?
colorless
55
In ONPG test, the suspension is incubated at ____°C, and positive results can generally be seen within _____ hours
35°C; 6
56
What test is performed if glucose is metabolized by the Mixed Acid Fermentation Pathway, stable acid end products are produced, which results in a low pH.
METHYL RED TEST
57
Reagent used in Methyl Red Test
Methyl Red
58
Positive Result in Methyl Red Test yields what color?
Red color
59
Negative Result in Methyl Red Test yields what color?
Yellow
60
What test detects the organism's ability to convert the acid products to acetoin and 2,3-butanediol
VOGES-PROSKAUER TEST
61
After incubation, what material is added first as a catalyst or color intensifier?
a-naphtol
62
Next, 40% _______ or ______(solution) is added
potassium hydroxide (KOH) or sodium hydroxide (NaOH)
63
Negative Result in Voges-Proskauer Test yields what color?
Yellow
64
Positive Result in Voges-Proskauer Test yields what color?
Red
65
What agar is used to differentiate gram-negative bacilli based on decarboxylation or deamination of lysine and the formation of hydrogen sulfide (H2S)
LYSINE IRON AGAR
66
pH indicator of Lysine Iron Agar
Bromcresol purple
67
Positive result in Lysine Deaminase yields what color?
Burgundy/Red
68
Negative result in Lysine Deaminase yields what color?
Purple
69
What element is formed on the Lysine Decarboxylase?
Cadaverine
70
Negative result in Lysine Decarboxylase yields what color?
Yellow (acidic-glucose is fermented)
71
Positive result in Lysine Decarboxylase yields what color?
Purple (presence of Cadaverine)
72
What are the deaminase-producing bacteria in Lysine Iron Agar?
Proteus Providencia Morganella
73
What are the H2S producer bacteria in Lysine Iron Agar?
Salmonella Arizona Citrobacter Edwardsiella
74
What test determines whether an organism possesses the enzyme that deaminates phenylalanine to phenylpyruvic acid.
PHENYLALANINE DEAMINASE (PAD) TEST
75
What is the purpose of Sulfide (H2S) in Sulfide Indole Motility Agar?
black precipitate
76
What is the purpose of indole in Sulfide Indole Motility Agar?
organisms that possess the enzyme trytophanase are capable of deaminating tryptophan with the formation of the intermediate degradation products of indole, pyruvic acid, and ammonia.

Clinical Bacteriology (Lab) (1 decks)

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