Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Clinical Bacteriology (Lab) > Biochemical ID of Gram (-) Bacteria > Flashcards

Biochemical ID of Gram (-) Bacteria Flashcards

1
Q

What family is also referred to as “enterics”

A

Family Enterobacteriaceae

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What family is gram-negative bacilli and coccobacilli (rods), non- spore forming, facultative anaerobic composition??

A

Family Enterobacteriaceae

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Family Enterobacteriaceae do not produce _____ except Plesiomonas

A

cytochrome C oxidase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Family Enterobacteriaceae are motile at body temperatures except what bacteria?

A

Klebsiella, Shigella and Yersinia

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

In the specimen collection of the laboratory diagnosis, fecal specimens or stool samples are collected, usually
within first ____ days of illness and ____ the
administration of antibiotic.

A

four (4); before

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

In the specimen collection of the laboratory diagnosis, Specimen should be processed within what time
of collection?; if not, place it to appropriate transport
media and kept in what temperature?

A

1-2 hours; 4 ̊C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

In the gram stain of the laboratory diagnosis, Direct smear examination of stool samples is not particularly
helpful in identifying enteric pathogens but may reveal the presence of _____

A

inflammatory cells

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Cultures grow at an optimal temperature of ______

A

35°C to 37°C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Cultures grow at an low temperatures of ______°C, example of a bacteria is _______

A

1-5;Serratia and Yersinia

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Cultures grow at an low temperatures of ______°C, example of a bacteria is _______

A

45-50°C; E. coli

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

To ensure isolation of both opportunistic and
fastidious pathogens, laboratories must provide
appropriate transport media, such as ________

A

Cary-Blair, Amies, or Stuart media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

All Enterobacteriaceae produce similar growth on ___ agars.

A

blood and chocolate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What bacteria may be mucoid because of their polysaccharide capsule

A

Klebsiella or Enterobacter

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What bacteria “swarm” on blood and chocolate agars.

A

P. mirabilis, P. penneri, and P. vulgaris

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Broth cultures of Y. pestis exhibit what characteristic?

A

“stalactite pattern”

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What bacteria produces bull’s-eye colonies?

A

Y. enterocolitica

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Agar that is best used to characterize gram negative rods

A

MacConkey AGAR

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

What agar is selective for gram-negative enteric bacilli

A

MacConkey AGAR

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Lactose fermenter have what color of colonies?

A

dark pink colonies

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Time of incubation of a Rapid Lactose Fermenter

A

18 to 24 hours

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Time of incubation of a Late Lactose Fermenter

A

48 to 72 hours

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Identify the bacteria if it is a Rapid or Late Lactose Fermenter: Escherichia

A

Rapid Lactose Fermenter

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

Identify the bacteria if it is a Rapid or Late Lactose Fermenter: Enterobacter

A

Rapid Lactose Fermenter

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

Identify the bacteria if it is a Rapid or Late Lactose Fermenter: Klebsiella

A

Rapid Lactose Fermenter

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

Identify the bacteria if it is a Rapid or Late Lactose Fermenter: Hafnia

A

Late Lactose Fermenter

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

Identify the bacteria if it is a Rapid or Late Lactose Fermenter: Citrobacter

A

Late Lactose Fermenter

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

Identify the bacteria if it is a Rapid or Late Lactose Fermenter: Serratia

A

Late Lactose Fermenter

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
28
Q

Identify the bacteria if it is a Rapid or Late Lactose Fermenter: Salmonella arizonae

A

Late Lactose Fermenter

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
29
Q

Identify the bacteria if it is a Rapid or Late Lactose Fermenter: Shigella sonnei

A

Late Lactose Fermenter

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
30
Q

Identify the bacteria if it is a Rapid or Late Lactose Fermenter: Yersinia enterocolitica

A

Late Lactose Fermenter

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
31
Q

Non-lactose fermenter have what color of colonies?

A

clear

32
Q

In the biochemical tests on carbohydrate utilization, what bacterium serve as transport enzyme to facilitate the lactose to enter the plasma membrane of the bacteria?

A

B-galactoside permease or lactose permease

33
Q

In the biochemical tests on carbohydrate utilization, what is the enzyme that hydrolyzes lactose
into glucose and galactose

A

B-galactosidase

34
Q

What fermenter possess both B-galactoside permease and B- galactosidase

A

LFs

35
Q

What fermenter do not possess either enzyme

A

NLFS

36
Q

What fermenter lack ẞ-galactoside permease but possess ẞ- galactosidase

A

LLFS

37
Q

OF Basal Medium produces what color on pH indicator?

A

brom thymol blue

38
Q

OF Basal Medium produces what color on uninoculated medium?

A

green

39
Q

OF Basal Medium produces what color on alkaline environment?

A

blue

40
Q

These reaction are produced by what element?
“Acid produced on both tubes”

A

oxidizer and fermenter

41
Q

These reaction are produced by what element?
“Acid only in closed tube”

A

fermenter and possible obligate anaerobe

42
Q

These reaction are produced by what element?
“Acid in open tube:”

A

Oxidizer

43
Q

What test is used to determine glucose and lactose or sucrose utilization and H2S production, gas production

A

TRIPLE SUGAR IRON (TSI)

44
Q

What is the composition of TSI?

A

10 parts lactose, 10 parts sucrose, 1 part glucose and
peptone

45
Q

What is the pH indicator of TSI?

A

Phenol Red - Yellow

46
Q

What is the ideal pH for the TSI to be called acidic?

A

pH 6.8

47
Q

What is the H2S indicator of TSI?

A

Ferrous sulfate and Sodium thiosulfate

48
Q

Reactions should be read within an ______ (time) incubation period; otherwise, erroneous results are
possible.

A

18- to 24-hour

49
Q

What is the fermenters present on A/A reaction on TSI agar

A

Lactose fermenter
Sucrose fermenter
Glucose fermenter

50
Q

What is the fermenters present on K/A reaction on TSI agar?

A

Glucose fermenter

51
Q

What is the fermenters present on K/K reaction on TSI agar?

A

No fermentation - not a member of
Enterobacteriaceae

52
Q

What are the H2S-producing bacteria in Triple Sugar Iron?

A

Salmonella
Proteus
Arizona
Citrobacter
Edwardsiella

53
Q

What test determine the ability of organism to produce B- galactosidase that hydrolyzes ONPG, a colorless compound, into galactose and o-nitrophenol, a
yellow compound (lactose fermenter)

A

ORTHO-NITROPHENYL-B-D-GALACTOPYRANOSIDE TEST (ONPG TEST)

54
Q

ONPG remains what color if the organism is an
NLF?

A

colorless

55
Q

In ONPG test, the suspension is incubated at ____°C, and
positive results can generally be seen within _____ hours

A

35°C; 6

56
Q

What test is performed if glucose is metabolized by the Mixed Acid Fermentation Pathway, stable acid end products are produced, which results in a low pH.

A

METHYL RED TEST

57
Q

Reagent used in Methyl Red Test

A

Methyl Red

58
Q

Positive Result in Methyl Red Test yields what color?

A

Red color

59
Q

Negative Result in Methyl Red Test yields what color?

A

Yellow

60
Q

What test detects the organism’s ability to convert the
acid products to acetoin and 2,3-butanediol

A

VOGES-PROSKAUER TEST

61
Q

After incubation, what material is added first as a
catalyst or color intensifier?

A

a-naphtol

62
Q

Next, 40% _______ or ______(solution) is added

A

potassium hydroxide (KOH) or sodium hydroxide (NaOH)

63
Q

Negative Result in Voges-Proskauer Test yields what color?

A

Yellow

64
Q

Positive Result in Voges-Proskauer Test yields what color?

A

Red

65
Q

What agar is used to differentiate gram-negative bacilli based on decarboxylation or deamination of lysine and the formation of hydrogen sulfide (H2S)

A

LYSINE IRON AGAR

66
Q

pH indicator of Lysine Iron Agar

A

Bromcresol purple

67
Q

Positive result in Lysine Deaminase yields what color?

A

Burgundy/Red

68
Q

Negative result in Lysine Deaminase yields what color?

A

Purple

69
Q

What element is formed on the Lysine Decarboxylase?

A

Cadaverine

70
Q

Negative result in Lysine Decarboxylase yields what color?

A

Yellow (acidic-glucose is fermented)

71
Q

Positive result in Lysine Decarboxylase yields what color?

A

Purple (presence of Cadaverine)

72
Q

What are the deaminase-producing bacteria in Lysine Iron Agar?

A

Proteus
Providencia
Morganella

73
Q

What are the H2S producer bacteria in Lysine Iron Agar?

A

Salmonella
Arizona
Citrobacter
Edwardsiella

74
Q

What test determines whether an organism possesses the enzyme that deaminates phenylalanine to
phenylpyruvic acid.

A

PHENYLALANINE DEAMINASE (PAD) TEST

75
Q

What is the purpose of Sulfide (H2S) in Sulfide Indole Motility Agar?

A

black precipitate

76
Q

What is the purpose of indole in Sulfide Indole Motility Agar?

A

organisms that possess the enzyme trytophanase are capable of deaminating tryptophan with the formation of the intermediate degradation products of indole, pyruvic acid, and ammonia.

Clinical Bacteriology (Lab) (1 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions