Biochemical Engineering Flashcards
A competitive inhibitor of an enzyme is usually
- a highly reactive compound
- a metal ion such as Hg²⁺ or Pb²⁺
- structurally similar to the substrate.
- water insoluble
structurally similar to the substrate.
Linear inhibition is sometimes called as
- complete inhibition
- incomplete inhibtion
- partial inhibition
- mixed inhibition
complete inhibition
The types of inhibition pattern based on Michaelis Menten equation are
- competitive
- non-competitive
- uncompetitive
- all of the above
all of the above
The effect of non-competitive inhibition on a Lineweaver-Burk Plot is that
it can move the entire curve to the right
it can change the y-intercept
it can change the x-intercept
all of these
it can change the y-intercept
The rate-determining step of Michaelis Menten kinetics is
the complex formation step
the complex dissociation step to produce product
the product formation step
Both (a)and(c)
the complex dissociation step to produce product
In competitive inhibition a factor is obtained from the measurement of
Vmax
KM
Y-intercept in Lineweaver-Burk Plot
None of these
KM
Which of these proteases is not a cysteine active site protease?
Calpain
Cathepsin D
Papain
None of the above
Cathepsin D
Given an enzyme with a Km = 10m M and Vmax = 100 m mol/min. If [S] = 100 m M, which of the following will be true?
A 10 fold increase in Vmax would increase velocity 10 fold y
A 10 fold decrease in Km would increase velocity
Both (a) and (b)
A 10 fold increase in Vmax would decrease velocity 20 fold
A 10 fold increase in Vmax would increase velocity 10 fold y
The conformational change in an enzyme after the substrate is bound that allows the chemical reaction to proceed, can be explained by
induced fit
transition
fit and fine
Pasteur
induced fit
The active site of an enzyme remains
at the center of globular proteins
rigid and does not change shape
complementary to the rest of the molecule
none of the above
none of the above
Which category of enzymes belongs to class two in the international classification?
Hydrolases
Ligases
Transferases
Isomerase
Transferases
The Woolf-Augusteinsson-Hofstee plot of ν versus ν/[S] and the Eadie-Scatchard plot of ν/[S] versus ν do not involve reciprocals of ν therefore are considered to be more reliable when the error in v is
non-significant
significant
nothing to do with the reliability
non significant in selected cases
significant
The relationship between Keq, Km and Vmax is known as
Haldane equation
Michaelis Menten equation
Numerical solution approach
Gibbs-Helmholtz equation
Haldane equation
The reciprocal equation for non competitive inhibition can be arranged to the equation for the
Dixon plot
Woolf-Augusteinsson-Hofstee plot
Eadie-Scatchard plot
Hanes-Woolf plot
Dixon plot
Which of the following statements is true for enzymatically catalyzed reaction?
The activation energy of the reaction is lowered so that a larger proportion of the substrate qualifies to overcome it
Additional substrate molecules are energized to overcome the activation energy of the reaction
The activation energy of the reaction is increased, thus decreasing the likelihood that any substrate molecules will overcome it
The activation energy of the reaction is lowered so that a fewer substrate molecules can overcome it
The activation energy of the reaction is lowered so that a larger proportion of the substrate qualifies to overcome it
Which of the following common drugs is not a specific enzyme inhibitor?
Iodine
Methotrexate
Sulfbnilamide
Penicillin
Iodine
The enzyme inhibition can occur by
reversible inhibitors
irreversible inhibitors
Both (a) and (b)
None of these
Both (a) and (b)
In a Lineweaver-Burk Plot, competitive inhibitor shows which of the following effect?
It moves the entire curve to right
It moves the entire curve to left
It changes the x-intercept
It has no effect on the slope
It changes the x-intercept
Which of the following statements is not true?
Enzymes are proteins that bind to specific substrates and increase the velocity of reactions involving those substrates
Enzymes function by overcoming the activation energy barrier of a reaction
Enzymes make thermodynamically favorable reactions to proceed; they cannot make unfavorable reactions to occur
Enzymes only function when they are in intact cells
Enzymes only function when they are in intact cells
Non-competitive inhibitor of an enzyme catalyzed reaction
decreases Vmax
binds to Michaelis complex (ES)
both (a) and (b)
can actually increase reaction velocity in rare cases
both (a) and (b)
An enzyme and a reactant molecule maintain relationship as
a temporary association
an association stabilized by a covalent bond
one in which the enzyme is changed permanently
non complementary binding
a temporary association
An enzyme is assayed at an initial substrate concentration of 2 x 10⁻⁵ M. In 6 minute, half of the substrate is used. The Km for the substrate is 2 x 10⁻³ M. The value of k in minute is
0.115
0.42
0.093
6.693
0.115
The plot commonly used for determining the value of Vmax is
Lineweaver Burk plot
Langmuir plot
Eadie Hofstee plot
all of these
all of these
Quasi steady state is also known as
Michaelis Menten approach
Briggs-Haldane approach
Pseudo steady state
all of the above
Pseudo steady state
Which of these enzymes contains a Zinc (Zn) ion?
Carboxypeptidase A
Phosphorylase B kinase
Tyrosine hydroxylase
Phosphodiesterase
Carboxypeptidase A
A noncompetitive inhibitor of an enzyme-catalyzed reaction
increases KM and increases Vmax
increases KM and reduces Vmax
reduces KM and increases Vmax
reduces KM and reduces Vmax
increases KM and reduces Vmax
An allosteric inhibitor of an enzyme usually
participates in feedback regulation
denatures the enzyme
is a hydrophobic compound
causes the enzyme to work faster
participates in feedback regulation
Which of the following activity is possible by transferases?
Transfer of methyl groups
Transfer of glycosyl group
Both (a) and (b)
None of these
Both (a) and (b)
A classical uncompetitive inhibitor is a compound that binds
reversibly to the enzyme substrate complex yielding an inactive ESI complex
irreversibly to the enzyme substrate complex yielding an inactive ESI complex
reversibly to the enzyme substrate complex yielding an active ESI complex
irreversibly to the enzyme substrate complex yielding an active ESI complex
reversibly to the enzyme substrate complex yielding an inactive ESI complex
Which graphical method is used to determine an enzyme degree of cooperativity?
Hill plot
Koshland curve
Michaelis-Menten hyperbola
Can not be determined
Hill plot
β-amylase is
endoenzyme
exoenzyme
saccharifying enzyme
both (b) and (c)
both (b) and (c)
Juice clarification extraction is facilitated by using
cellulases
amylase
inulinase
lactase
cellulases
Lysozyme is naturally present in
egg white
bacteria
tears & milk
all of these
all of these
Enzymes act as antimicrobials
by depriving an organism of a necessary metabolite
by generating a substances toxic to the organism
by attracting a cell wall component
all of the above
all of the above
Trichoderma β-glucanase is reported
to stabilize mashing
to convert taste fractions of dextrins to fermentable sugars in beer
to convert starch to dextrin
all of the above
to stabilize mashing
The bitter taste of the high protein materials is reduced by using
invertase
dectinase
protease
none of these
protease
Sulphydryl oxidase is used for
discoloration
clarification of images
UHT milk off flavour removal
all of these
UHT milk off flavour removal
α-amylase is an endo enzyme which requires
Ca
Cu
Mn
None of these
Ca
Liquefaction of starch to dextrin is carried out by
α-amylase
cellulase
pectinase
all of these
α-amylase
Which is true about rennet?
It is a mixture of protease chymosin and pepsin
It is a mixture of rennin and pepsin
Both (a) and (b)
none of the above
Both (a) and (b)
Milk digestibility is improved by using
RNase
lactase
β-amylase
none of these
lactase
Which of the following mainly serve to convert starch into high fructose corn syrup (HFCS)?
α-amylase
Gluco-isomerase
Gluco-amylase
all of these
all of these
Lysozyme
catalyses hydrolysis of β-1-4 linkages between N-acetyl muranic acid and N-acetyl glucosamine in peptideoglycan
catalyses hydrolysis of α-1-4 linkages between N-acetyl muranic acid and N-acetyl glucosamine in peptideoglycan
catalyses hydrolysis of α-1-4 linkages between N-diacetyl muranic acid and N-diacetyl glucosamine in peptideoglycan
all of the above
catalyses hydrolysis of β-1-4 linkages between N-acetyl muranic acid and N-acetyl glucosamine in peptideoglycan
Hersperidinase is used for
juice clarification
juice debittering
off flavour reduction
RNA reduction in fish
juice clarification
Which of the following metallic ion is there in ascorbic acid oxidase?
Mg
Fe
Cu
Mn
Cu
Which of the enzyme combination is commercially used for the removal of oxygen?
Glucose oxidase-cellulase
Glucose oxidase-catalase
Glucose oxidase-lactase
All of these
Glucose oxidase-catalase
The enzyme used to reduce bitterness of grapes commonly contains
α-L-rhamonosidase
β-d-glucosidase
β- galactosidase
both (a) and (b)
both (a) and (b)
Citrus juice debittering can be carried out using
limoninase
inulinase
anthocyanase
None of these
limoninase
Which of the following enzyme is responsible for causing vitamin B deficiency disease beriberi?
Ascorbic acid oxidase
Thiaminase
Lipoxygenase
None of these
Thiaminase
Soya off flavour removal may be achieved using
di acetyl reductase
β- amylase
aldehyde oxidase
protease
aldehyde oxidase
The reduction in off flavour of beer is practiced through
hersperidinase
rnase
invertase
diacetyl reductase
diacetyl reductase
Discoloration can be achieved by using
sulphloydryl oxidase
proteases
anthocyanase
all of these
anthocyanase
The prosthetic group present in phenolase enzyme is
Mg
Cu
Ca
Fe
Cu
Enzymes degrade, alter or synthesize a food component through
oxidation/reduction/isomerization
hydrolysis/synthesis
group transfer
all of the above
all of the above
The enzyme β-galactosidase is also known as
lactase
EC3.2.1.23
both (a) and (b)
isomerase
both (a) and (b)
Chymosin hydrolyses the bond between
Alanine and glycine
Phenyl alanine and methonine
Glutamic acid and alanine
Alanine and phenyl alanine
Phenyl alanine and methonine
The ratio of the amount of a protein present in a sample, which is used as a measure of purification, is known as
specific activity
relative activity
purity ratio
all of these
specific activity
If a reaction occurs in the absence of inhibitor with rate ν0 and in the presence of inhibitor with rate νi, the degree of inhibition is defined as
(ν0 - νi)/ν0
(ν0 + νi)/ν0
(ν0νi)/ν0
(ν0-νi)/νi
(ν0 - νi)/ν0
The rate equation in competitive inhibition based on Michaelis Menten equation is given by
rmaxS/(Km (1+I/Ki)+S))
rmaxE/(Km (1+I/Ki)+S))
rmaxI/(Km (1+I/Ki)+S))
rmaxS/(Km (1+I/Ki))
rmaxS/(Km (1+I/Ki)+S))
Classical noncompetitive inhibition is obtained only under
slow equilibrium conditions
moderate equilibrium conditions
rapid equilibrium conditions
non-equilibrium conditions
rapid equilibrium conditions
In the steady state the material balance equation for any component of a system is
rate of addition + rate of removal - rate of formation = 0
rate of addition - rate of removal + rate of formation = 0
rate of addition + rate of removal + rate of formation = 0
none of the above
rate of addition - rate of removal + rate of formation = 0
For an enzyme that displays Michaelis-Menten kinetics, the reaction velocity (as a fraction of Vmax) observed at [S] = 2 KM will be
0.09
0.33
0.66
0.91
0.66
Predominantly uncompetitive inhibition may be called when
competitive inhibition is greater than uncompetitive inhibition
competitive inhibition is smaller than uncompetitive inhibition
competitive inhibition is equal to uncompetitive inhibition
none of the above
competitive inhibition is greater than uncompetitive inhibition
An enzyme has a Km of 4.7 x 10-5M. If the Vmax of the preparation is 22m moles liter-1 min-1, what velocity would be observed in the presence of 2.0 x 10-4M substrate and 5.0 x 10-5M of a competitive inhibitor?
13.54μ moles liter-1min-1
6.68μ moles liter-1min-1
7.57μ moles liter-1min-1
17.8μ moles liter-1min-1
13.54μ moles liter-1min-1
The rate equation in non-competitive inhibition based on Michaelis Menten equation is given by
rmaxS/(Km + S)(1+I/Ki)
rmaxE/(Km (1+I/Ki)+S))
VmaxS/(Km + S)(1+I/Ki)
rmaxS/Km
rmaxS/(Km + S)(1+I/Ki)
Which of the following statement(s) regarding enzymes, is/are false?
Enzymes are always proteins that function as catalysts
Enzymes provide activation energy for reactions
Enzyme activity can be regulated
Enzymes may be used many times for a specific reaction
Enzymes provide activation energy for reactions
The slope of Lineweaver Burk plot for Michaelis Menten equation is
Vmax/Km
Km/Vmax
1/Km
Km.Vmax
Km/Vmax
The initial velocity, V0, of an enzyme catalyzed reaction reaches Vmax as
[S] = KM
[S] = 10 * KM
1/[S] = 1/KM
1/[S] → 0
1/[S] → 0
The usual method(s) to solve rate equation of simple enzyme kinetics is/are
Michaelis Menten approach
Briggs-Haldane approach
Numerical solution approach
all of these
all of these
Michaelis Menten equation can also be written as
(-Cs)/r = (Cs/rmax)+(Km/rmax)
1/r = (1/rmax)+(Km/(rmax.Cs))
r = rmax-(Km.r/Cs)
All of these
All of these
Which of the following step is assumed to be the slowest step in the Michaelis Menten equation?
The substrate consuming step
The product releasing step
Formation of enzyme substrate complex
None of these
The product releasing step
When substrate [S] = KM (Michaelis-Menten constant), the velocity of an enzyme catalyzed reaction is about
0.1 * Vmax
0.2 * Vmax
0.5 * Vmax
0.9 * Vmax
0.5 * Vmax
A classical noncompetitive inhibitor has
no effect on substrate binding
no effect on substrate binding and vice versa
significant effect on substrate binding
significant effect on substrate binding and vice versa
no effect on substrate binding and vice versa
The active site of an enzyme differs from an antibody-antigen binding site in that the enzyme active site
contains modified amino acids
catalyzes a chemical reaction
is complementary to a specific ligand
contains amino acids without side chains
catalyzes a chemical reaction
Enzymes are basically
proteins
vitamins
fat
carbohydrates
proteins
Which of the following refers to pseudo steady state ?
d(CE)/dt = 0
d(Cp)/dt = 0
d(CES)/dt = 0
d(Cs)/dt = d(CES)/dt
d(CES)/dt = 0
Most enzymes work by
increasing energy of activation
decreasing energy of activation
not affecting energy of activation
none of the above
decreasing energy of activation
Which category of enzymes belongs to class 5 in the international classification?
Hydrolases
Isomerases
Oxido-reductases
Cyclase
Isomerases
Lock and key theory is based on the compatibility of
enzyme and substrate
enzyme and product
enzyme and enzyme substrate complex
enzyme substrate complex and product
enzyme and substrate
When an enzyme is functioning at Vmax, the rate of the reaction is limited by
the number of collisions between enzyme and substrate
the number of substrate molecules in the reaction
the concentration of the substrate
the rate at which the enzyme can convert substrate to product
the rate at which the enzyme can convert substrate to product
The equation for the rate of product formation for simple enzyme reaction is given by (Where rmax, maximum reaction rate, Cs substrate concentration, Cp product concentration ES, CES enzyme-substrate concentration)
rp = rmax Cs/(Km+Cs)
rp= rmax CES/(Km+ CES)
rp = rmax Cs/(Km+CES)
rp = rmax Cs/(Km+Cp)
rp = rmax Cs/(Km+Cs)
When [S] = 0.1 *KM, the velocity of an enzyme catalyzed reaction is about:
0.1 * Vmax
0.3 * Vmax
0.5 * Vmax
0.7 * Vmax
0.1 * Vmax
The function of the disengagement zone in an airlift fermenter is to
prevent CO2 rich bubbles from entering the downcomer
reduce the velocity of the bubbles
reduce liquid loss as aerosols
all of the above
all of the above
Stationary phase is described as
no further increase in the cell population after a maximum value
deceleration of growth and division rate after the growth rate reaches a maximum
acceleration of growth and division rate after the growth rate reaches a maximum
deceleration of growth and division rate after the growth rate reaches a minimum
no further increase in the cell population after a maximum value
In the accelerated phase, cell starts to
increase and the division rate increases to reach a maximum
decrease and the division rate increases to reach a maximum
increase and the division rate decreases to reach a maximum
increase and the division rate increases to reach a minimum
increase and the division rate increases to reach a maximum
The monod model predicts that the specific growth rate
will increase with the concentration of the growth limiting substrate until it reaches a maximum value
will decrease with the concentration of the growth limiting substrate
will increase with the concentration of the growth limiting substrate
does not depend on growth limiting substrate
will increase with the concentration of the growth limiting substrate until it reaches a maximum value
A mixed fermentation is one, which produces
both alcohol and carbon dioxide
both acid and carbon dioxide
both acid and alcohol
several different kinds of acid
both acid and alcohol
The function of a mechanical seal is to
prevent contaminants entering the reactor
prevent cells from leaving the reactor
both (a) and (b)
prevent air to enter
both (a) and (b)
The phenomenon in which substrates are used in a sequential manner is known as
trans-substrate genesis
dialism
diauxie
multiplicity
diauxie
The dilution rate, D is defined as(where F = volumetric flow rate, VR = total volume of culture in the reactor and μ specific growth rate)
F/VR
VR/F
μ/F
F/μ
F/VR
Diauxie is
growth factors
microbiological die off
the simultaneous uptake of nutrients
the stagewise uptake of nutrients
the stagewise uptake of nutrients
An open system in which the growth rate is maintained by adding a nutrient (present in limiting quantities) at the same rate as that medium containing micro-organisms is removed is called
manostat
chemostat
turbidostat
culturostat
chemostat
In the death phase
nutrients available for the cells are depleted and begin to die
the number of viable cells will increase
nutrients available for cells are replenished and start to multiply
none of the above
nutrients available for the cells are depleted and begin to die
The maximum specific growth rate of an organism depends on
medium composition
temperature
pH
All of these
All of these
Which of the following is not correct for the Monod model and the Michaelis Menten Model?
The Michaelis Menten Model was derived from a curve fitting exercise
The Michaelis Menten model was derived from an analysis of the mechanism of microbial growth
The Monod model was derived from an analysis of the mechanism of microbial growth
All of the above
The Monod model was derived from an analysis of the mechanism of microbial growth
Bubble column reactor has
large height to diameter ratio
small height to diameter ratio
large diameter to height ratio
small diameter to height ratio
large height to diameter ratio
Why a T-flask used in small-scale cell culture is incubated in a horizontal position?
To save space
To increase the surface area of the liquid-air interface
Both (a) and (b)
To increase the rate of oxygen transfer into the liquid
Both (a) and (b)
An open system in which the growth rate is maintained by the removal and addition of media at such a rate as to maintain a constant cell density is called a
manostat
chemostat
turbidostat
culturostat
turbidostat
Wash out in steady state fermentation occurs when
dilution rate is less than maximum specific growth rate
dilution rate is higher than the maximum specific growth rate
cell concentration reaches the maximum
specific growth rate is maximum
dilution rate is higher than the maximum specific growth rate
Fermentor should be filled with medium upto
65-70%
70-75%
75-80%
80-85%
75-80%
Bacterial growth curve is obtained by plotting
number of cells versus time
number of spores versus time
log of number of cells versus time
log of number of cells survived versus time
log of number of cells versus time
When intracellular enzymes of whole cells are to be used in a bio-conversion process, it is often necessary to____________the cells.
permeabilize
lyophilize
heat-kill
denature
permeabilize
Heat transfer rates (per unit volume) will be lowest in
Stirred tank bioreactor with biomass recycle
Continuous air lift bioreactor
Continuous packed bed reactor
Continuous fluidized bed bioreactor
Continuous packed bed reactor
The function(s) of the draft tube in an airlift bioreactor is
to reduce bubble coalescence
to improve circulation
to even out shear conditions throughout the reactor
all of the above
all of the above
The Monod Model relates
substrate utilized with the biomass consumption
specific growth rate to the substrate availability
yield with the biomass utilization
the biomass concentration with specific growth rate
specific growth rate to the substrate availability
The height to diameter ratio (H/D) for the column fermenters is
<3
>3
<1.5
>1.5
> 3
The high oxygen transfer efficiencies of airlift bioreactors is due to
the large aspect ratio of the reactor, which leads to a high gas hold-up
the large aspect ratio of the reactor, which leads to a high oxygen solubility at the base of the reactor
the draft tube, which reduces bubble coalescence in the reactor
all of the above
all of the above
The maximum specific growth rate (μm) of an organism in batch culture is equal to slope of a plot of
In [biomass] against time for exponential phase data only
In [biomass] against time
biomass against time
biomass against time for stationary phase data only
In [biomass] against time for exponential phase data only
The region of an airlift bioreactor in which the liquid travels in a downward direction is called the
downcomer
disengagement zone
air riser
flotsam
downcomer
Population doubling time, td can be expressed as (where μ is the specific growth rate.)
log2/μ
ln2/μ
μ/ln2
μ/log2
ln2/μ
A higher Ks value of Monod’s equation means
greater affinities to substrate
lower affinities to substrate
unaffected with the substrate bonding
lower dissociation constant value
greater affinities to substrate
The increased air flow rate in bubble column fermenter can cause excessive foaming and high retention of air bubbles in the column which
decreases the productivity of the fermenter
increases the productivity of the fermenter
modestly increases the productivity of the fermenter
does not affect on the productivity
decreases the productivity of the fermenter
Which of the following is correct for the prediction of time requirement in a batch fermenter?
Carrying out experiments
Mathematical modelling of the fermentation process
Both (a) and (b)
It is impossible to predict the time required for fermentation
Both (a) and (b)
In an airlift bioreactor, the spent gases released from the liquid are called
downcomer
disengagement zone
air riser
flotsam
disengagement zone
The contents in jet loop reactor are mixed through
compressed air
a mechanical agitator
external pumping
any of these
external pumping
The specific growth rate (μ) is defined as
the concentration of biomass in the reactor
rate of increase of total biomass in a reactor
the rate of individual cells division or increase in their biomass
the rate of cell death
the rate of individual cells division or increase in their biomass
The number of baffles in a standard stirred tank bioreactor is
8
6
4
2
4
In the stationary phase
growth is proportional to death
growth rate is equal to death rate
growth rate is lesser than death rate
no co-relation exist between death rate and growth rate
growth rate is equal to death rate
Which of the following is an example of a mathematical model?
Volume of cuboid = length x breadth x height
The Monod Model
The Michaelis Menten Equation
All of the above
All of the above
Biomass concentrations during fermentation is
diluting the samples to optical density less than 0.3
monitored by controlling the changes in biomass concentrations
considering that optical density is proportional to the surface area of the biomass in the sample
all of the above
all of the above
Chemostats work on the principle of
maintaining constant volume of culture medium
maintaining continuous flow of nutrients
maintaining uniform nutrients concentration
operating at higher pressure
maintaining uniform nutrients concentration
During the exponential phase the maximum specific growth rate equals specific growth rate as
concentration of the growth limiting substrate is much less than the monod constant
concentration of growth limiting substrate is much greater than the monod constant
specific growth rate increases exponentially
concentration of the growth limiting substrate is equal to the monod constant
concentration of growth limiting substrate is much greater than the monod constant
An internal draft tube is preferrred to an external draft tube because
it is easier to clean and sterilize a reactor
it is cheaper to build a reactor with an internal draft tube
Both (a) and (b)
none of the above
it is easier to clean and sterilize a reactor
A functional relationship between the specific growth rate and essential compounds concentration was proposed by Monod in
1958
1946
1942
1950
1942
The optical density sometimes drops during the lag phase because
cells tend to grow without dividing leading to a decrease in the surface area to volume ratio
of fast cell growth causing them to fill up with DNA
cells swell up with water
cells swell up with RNA
cells tend to grow without dividing leading to a decrease in the surface area to volume ratio
The application of just in time (JIT) manufacturing techniques in biotechnology is important because
raw materials used in biotechnology are biodegradable
the products of biotechnology are biodegradable
it is a very competitive business (d) all of the above
all of the above
all of the above
Residence time distribution (RTD) of a reactor is independent of
micro mixing
volume of reactor
height of the reactor
width of the reactor
micro mixing
The bubble column fermenter is usually limited to
aerobic fermentations
anaerobic fermentations
Both (a) and (b)
facultative fermentation
aerobic fermentations
A culture in a closed vessel to which no additional medium is added and from which no waste products are removed is called a __________ culture.
continuous
batch
fed-batch
semi continuous
batch
If the specific growth rate, μ. is constant with time during the exponential growth period, the equation correlating bacterial number density Cn), cell number concentration with respect to time can be expressed as
Cn= Cn0 exp[μ (t - t0)]
Cn0 = Cn exp[μ (t - t0)]
Cn/Cn0 =(1/μ)exp(t-t0)
Cn0 / Cn = (1/μ)exp(t-t0)
Cn= Cn0 exp[μ (t - t0)]
The diameter of the baffles in a standard stirred tank bioreactor ranges from
1/10th - 1/12th of the tank diameter
1/3rd - 1/4th of the tank diameter
1/15th - 1/20th of the tank diameter
1/20th - 1/25th of the tank diameter
1/10th - 1/12th of the tank diameter
During the log phase cell numbers increase exponentially
because cells produce enzymes
because cells do not have cell membranes
because cells are autocatalysts
because the rate of cell division increases exponentially
because cells are autocatalysts
Lag phase occurs mainly due to
acclimatization with new environment and substrate
higher growth temperature
substrate disliking
variation in substrate concentration
acclimatization with new environment and substrate
In a batch reactor specific growth rate
remains constant throughout the process
increases exponentially
changes during the fermentation
is highest during the stationary phase
changes during the fermentation
Turbidostat is recommended when continuous fermentation needs to be carried out at
high dilution rates near the washout point
low dilution rates near the washout point
moderate dilution rates near the washout point
any intensity of dilution rates near the washout point
high dilution rates near the washout point
The advantage of airlift bioreactors over stirred tank bioreactors of a similar size is
higher oxygen transfer rates
more uniform shear conditions
both (a) and (b)
less foaming
both (a) and (b)
Stirred tank fermenter (STF) can be employed for
aerobic fermentation of a wide range of cells including microbial, animal and plant cells
anaerobic fermentation of a wide range of cells including microbial, animal and plant cells
Both (a) and (b)
anaerobic fermentation of plant cells only
Both (a) and (b)
The specific growth rate is affected by
sabstrate concentration
product concentration
oxygen supply
all of these
all of these
The main function(s) of a draft tube in an air lift fermenter is/are to
increase the solubility of oxygen
distribute shear forces throughout the reactor and minimise bubble coalescence
concentrate shear forces around the sparger
concentrate shear forces near the disengagement zone
distribute shear forces throughout the reactor and minimise bubble coalescence
Which of the following is incorrect?
The mechanical agitation and aeration are effective for suspension of cells
The mechanical agitation and aeration are effective for mixing the medium
STF can’t be used for high viscosity medium
The mechanical agitation and aeration are effective for oxygenation
STF can’t be used for high viscosity medium
The contents in bubble column and air lift reactor are mixed through
compressed air
a mechanical internal moving part
external pumping
any of these
external pumping
Which of the following statements would be appropriate while comparing bubble column and air lift fermenter of the same size?
The average bubble size is smaller in an airlift bioreactor
Oxygen transfer rate is higher in an airlift bioreactor
The surface area for gas exchange is higher in an airlift bioreactor
All of the above
All of the above
During the lag phase
cells just start growing
cells synthesize new proteins and membrane components
cells produce antibiotics to kill competing organisms
cells simply grow slowly
cells synthesize new proteins and membrane components
Concentration of the growth limiting substrate means
trace elements concentration
the concentration of the substrate that controls the growth rate of the cells
sugar concentration in the fermentation medium
dissolved oxygen concentration
the concentration of the substrate that controls the growth rate of the cells
Tower fermentors are used for
continuous penicillin production
continuous beer production
production of enzymes
batch production of beer
continuous beer production
n wastewater treatment, which bioreactor is generally used?
Plug flow reactor
Trickle bed reactor
Tower fermentor
CSTR
Trickle bed reactor
The contents in stirred tank rector are mixed through
compressed air
a mechanical agitator
external pumping
any of these
a mechanical agitator
The height to diameter ratio (H/D) for the tank fermenters is
<3
>3
<1.5
>1.5
<3
The relatively large volume (10 to 40% of total fermenter volume) of the seed culture is employed to
increase the overall fermentation time
increase the overall yield of biomass
decrease the overall fermentation time
decrease the overall yield of product
decrease the overall fermentation time
In an airlift bioreactor, the air sparging region is called
downcomer
disengagement zone
air riser
none of these
air riser
The air riser is located on the outside of the draft tube for the large-scale airlift bioreactors because of
higher oxygen transfer rates
a small sparge ring
better heat transfer efficiencies
all of these
better heat transfer efficiencies
At the end of the lag phase, when growth begins, the division rate increases gradually and reaches a maximum value in the exponential growth period. This transitional period is commonly called the accelerated growth phase and is often included as a part of the
lag phase
stationary phase
death phase
exponential growth phase
lag phase
Which of the following(s) is/are considered the limitations of the exponential growth model?
Only predicts that cells grow exponentially during the log phase
It does not consider factors that causes cells growth to stop or slow
Predicts cell growth at a linear rate
All of the above
It does not consider factors that causes cells growth to stop or slow
To describe components of an airlift bioreactor which of the follow-ings are generally included?
Disengagement zone, air riser, downcomer
Flotsam, jetsam, downzone
Air comer, disentanglement zone, downsizer
All of the above
Disengagement zone, air riser, downcomer
The viscosity of liquid was classified as both dilatant and rheopectic, which means that the viscosity
increased with stirring speed but decreased with mixing time
increased with stirring speed and increased with mixing time
decreased with stirring speed but increased with mixing time
decreased with stirring speed and decreased with mixing time
increased with stirring speed and increased with mixing time
Which of the following describes the detrimental effect of bubbles on animal cells?
Cells trapped on the surface of bubbles can be damaged when the bubbles explode, releasing their pressure and surface energies
Cells trapped in the moving foam can be dragged apart by the bubbles
Both (a) and (b)
Bubbles can explode in the liquid medium causing the release of ultraviolet radiation
Both (a) and (b)
A fluid in which the viscosity decreases with increasing stirrer speed and mixing time, can be represented as
newtonian fluid
pseudoplastic, thixotropic fluid
dilatant, rheopectic fluid
dilatant, pseudoplastic fluid
pseudoplastic, thixotropic fluid
Filamentous fungal cells are more shear sensitive than bacterial cells because of
the presence of citin in cell walls of filamentous fungi
larger in size than bacterial cells and therefore are more susceptible to shear forces
fungal cells are much larger than bacterial cells
all of the above
all of the above
The viscosity of water at STP is approximately
0.005 kg.m-1.s-1
0.001 kg.m-1.s-1
0.003 kg.m-2.s-1
0.1 g.cm-1.s-1
0.001 kg.m-1.s-1
Bubble free aeration systems requires
silicone tubing or gas permeable membranes to facilitate gas transfer
carbon dioxide instead of air to supply oxygen
silicone tubing or gas permeable membranes to remove carbon dioxide by oxygen in the form of hydrogen peroxide
filled headspace of the reactor with carbon dioxide
silicone tubing or gas permeable membranes to facilitate gas transfer
Non-linear relationship between shear stress and shear rate exists in
newtonian fluids
non-newtonian fluids
both (a) and (b)
none of these
non-newtonian fluids
If the viscosity of a fluid decreases with increasing stirrer speed, then the nature of the fluid is
newtonian
pseudoplastic
dilatant
thixotropic
pseudoplastic
A pseudoplastic fluid is one in which the viscosity
is constant regardless of the stirrer speed or mixing time
changes during mixing but returns to its original state after mixing
increases with increasing stirrer speed
decreases with increasing stirrer speed
decreases with increasing stirrer speed
A viscoelastic fluid is one in which the viscosity
is constant regardless of the stirrer speed or mixing time
changes during mixing but returns to its original state after mixing
increases with increasing stirrer speed
decreases with increasing stirrer speed
changes during mixing but returns to its original state after mixing
Mixing in stirred tank reactors can
be described as either laminar, transient or turbulent
achieve turbulent conditions by increasing the stirrer speed
induce laminar flow conditions by the addition of baffles
all of the above
be described as either laminar, transient or turbulent
In animal cell bioreactor, bubble damage can be minimized by
the addition of a shear protectorant
using a bubble free oxygen delivery system
headspace aeration
all of the above
all of the above
The viscosity of a fluid changes with either stirrer speed or mixing time. But when mixing ceases, the viscosity returns to its original state. This fluid is best described as
newtonian
rheopectic
viscoelastic
thixotropic
viscoelastic
In sparged mammalian cell bioreactors, the cell damage is caused because of
shear stress arising during mixing of the cell culture fluid
shear stresses arising from the breakup of bubbles at the liquid surface
shear stresses between bubbles in foam at the liquid surface
all of the above
shear stress arising during mixing of the cell culture fluid
The SI unit for viscosity is
kg.m-1.s-1
kg.m-2.s-2
kg.m-3.s-1
kg.m-1.s-2
kg.m-1.s-1
The viscous nature of a non-Newtonian fluid
remains constant with time and power input
changes with the level of power input and/or the length of time during which the fluid is mixed
is similar to that of a perfect gas
is similar to that of water
changes with the level of power input and/or the length of time during which the fluid is mixed
Which of the following compound, if added to a solution will lead to the greatest increase in viscosity?
A globular protein
Glucose
A long chain protein such as albumin
Water
A long chain protein such as albumin
A Brookfield viscometer measures viscosity by determining
the shear stress
the shear rate
the shear stress and shear rate
the torque required to move an impeller at a set speed
the torque required to move an impeller at a set speed
Which of the following factors will affect the sensitivity of animal cells to shear conditions?
Cell size
Availability of nutrients in the medium
The presence of shear protectorants in the medium
All of the above
All of the above
The best described action of Pluronic F68 (non-ionic detergent) in a cell culture medium is that, it
acts on the surface properties of the cells and the culture medium
provides nutrients for the cells
acts as a flocculant
acts as a coagulant
acts on the surface properties of the cells and the culture medium
Water is a
newtonian fluid
pseudoplastic
dilatant
rheopectic
newtonian fluid
Which of the following affect the Kolmogorov eddy size?
Stirrer speed
Physical properties of the liquid
Size and type of impeller
All of these
All of these
A liquid is flowing at 11,400 l.h-1 along a pipeline having a diameter of 4 cm. If the liquid has a density of 1 g.ml-1 and viscosity of 0.001kg.m-1.s-1, then the liquid would be in the
laminar phase
transient phase
turbulent phase
any of the phase
turbulent phase
The viscosity of a fluid increases with the length of time that it is being mixed. This fluid would be best described as being
newtonian
rheopectic
viscoelastic
thixotropic
rheopectic
Which of the following can act as a shear protectorant?
Serum
Silicone oil
Glucose
Glutamic acid
Serum
The viscosity of a fluid increases with increasing stirrer speed. This fluid would be best described as being
newtonian
pseudoplastic
dilatant
thixotropic
dilatant
A dilatant fluid is one in which the viscosity
is constant regardless of the stirrer speed or mixing time
changes during mixing but returns to its original state after mixing
increases with increasing stirrer speed
decreases with increasing stirrer speed
increases with increasing stirrer speed
A rheopectic fluid is one in which the viscosity
is constant regardless of the stirrer speed or mixing time
changes during mixing but returns to its original state after mixing
increases with mixing time
decreases with mixing time
increases with mixing time
A fluid in which the viscosity increases with increasing stirrer speed and mixing time can be called as
newtonian fluid
pseudoplastic, thixotropic fluid
dilatant, rheopectic fluid
dilatant pseudoplastic fluid
dilatant, rheopectic fluid
The medium will have the highest viscosity (considering that the cells do not secrete gums) with the presence of
single celled bacteria
filamentous fungi that form pellets
filamentous fungi that grow as long poorly branched chains
yeast
filamentous fungi that grow as long poorly branched chains
A Newtonian fluid is one in which the viscosity
is constant regardless of the stirrer speed or mixing time
changes during mixing but returns to its original state after mixing
increases with increasing stirrer speed
decreases with increasing stirrer speed
is constant regardless of the stirrer speed or mixing time
Which pattern of mixing is preferred in stirred tank reactors during the culture of aerobic microorganisms?
Laminar flow
Transient flow
turbulent flow
all of these
all of these
Animal cell cultures do not have much effect on shear forces because
the cell size is generally smaller than the critical eddy size at which shear will have an effect
cells are larger than the critical eddy size at which shear will have an effect
animal cells have cell walls
animal cells have cell membranes
the cell size is generally smaller than the critical eddy size at which shear will have an effect
The viscosity of the culture fluid is classified as both pseudoplastic and thixotropic which indicates that the viscosity
increases with stirring speed but decreases with mixing time
increases with stirring speed and increases with mixing time
decreases with stirring speed but increases with mixing time
decreases with stirring speed and decreases with mixing time
decreases with stirring speed and decreases with mixing time
Pluronic F68 is added to cell culture media as
a nutrient
a shear protectorant
to increase oxygen transfer rates
all of these
a shear protectorant
A stirred tank reactor containing a fluid with a density of 2.5 g.ml-1 and viscosity of 0.002 kg m-1s-1 is mixed at 120 rpm. If the fluid is mixed with an impeller having diameter 50 cm, then the Reynolds number of the liquid in the reactor is
2500
100
12,50,000
1,000,000
12,50,000
If the viscosity of a fluid decreases with the length of time that it is being mixed, then this fluid is
newtonian
rheopectic
viscoelastic
thixotropic
thixotropic
Which of the following describes the importance of the Kolmogorov eddy size in the biotechnology?
The Kolmogorov eddy size is a measure of the smallest eddy size in a liquid
When the Kolmogorov eddy size become equivalent or smaller than the cell or particle diameter, then the cell or particle will become susceptible to shear damage
At the Kolmogorov eddy size, much of the energy of the originating eddy will be released
All of the above
All of the above
A thixotropic fluid is one in which the viscosity
is constant regardless of the stirrer speed or mixing time
changes during mixing but returns to its original state after mixing
increases with mixing time
decreases with mixing time
decreases with mixing time
Newtonian fluid is one in which the viscosity
remains constant with time and power input
increases with the level of power input
decreases with the level of power input
drastically increases with the level of power input
remains constant with time and power input
Reynolds number for laminar flow over a plate should be less than
500 mm
5 x 10⁻⁵
5 x 10⁵
5 m
5 x 10⁵
The approach which is commonly used to determine the level of damage to animal cells in a culture medium is to monitor
the concentration of lactate in the medium
the level of lactate oxidase activity in the medium
the level of lactate dehydrogenase activity
the level of lactase activity in the medium
the level of lactate dehydrogenase activity
A Brookfield viscometer cannot determine the shear stress because
the shear stress is not defined
a computer is required
the impeller type is given only by numbers
all of the above
the shear stress is not defined
Which of the following is correct?
The viscosity of liquids and gases decrease with increasing temperature
The viscosity of liquids and gases increase with increasing temperature
the viscosity of gases increases with increasing temperature, but decreases in liquids
the viscosity of gases decreases with increasing temperature, but decreases in liquids.
the viscosity of gases increases with increasing temperature, but decreases in liquids
The Komologorov eddy size
is constant at all stirrer speeds
increases with stirrer speed
decreases with stirrer speed
becomes zero when the stirrer speed is very low
decreases with stirrer speed
Pluronic F68 protects mammalian cells from damage stems as it is a/an
surface active agent which stabilizes foams
surface active agent which destabilizes foams
excellent source of vitamins
coagulant
surface active agent which stabilizes foams
Reynolds number for turbulent flow over a plate should be more than
5 x 10⁻⁵
106
5 x 10⁵
500
106
Concentration polarization can be reduced further by
pre filtering the solution
reducing the flow rate per unit membrane surface area
back washing periodically
all of the above
all of the above