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Microbiology summer 2015 Lab practical > Biochem lab chart > Flashcards

Biochem lab chart Flashcards

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1
Q

Oxidation/Ferm

  • Test
  • Media
  • pH Indicator /Reagent
  • Substrate
  • Enzyme
  • Appearance if positive
  • Appearance if negative
A
  • OF media
  • Bromthymol Blue
  • Glucose
  • n/a
  • yellow
  • blue/green
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2
Q

Phenol Red broth Fermentation

  • Test
  • Media
  • pH Indicator /Reagent
  • Substrate
  • Enzyme
  • Appearance if positive
  • Appearance if negative
A
  • PR media
  • phenol red
  • Glucose, Lactose or Succrose
  • n/a
  • yellow
  • red/pink
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3
Q

TSI Fermentation

  • Test
  • Media
  • pH Indicator /Reagent
  • Substrate
  • Enzyme
  • Appearance if positive
  • Appearance if negative
A
  • TSI media
  • phenol red
  • Glucose, Lactose and Succrose
  • n/a
  • yellow
  • red/pink
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4
Q

Gelatin hydrolysis

  • Test
  • Media
  • pH Indicator /Reagent
  • Substrate
  • Enzyme
  • Appearance if positive
  • Appearance if negative
A
  • gelatin media
  • n/a
  • gelatin
  • gelatinase
  • liquified
  • solidified
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5
Q

Urea hydrolysis

  • Test
  • Media
  • pH Indicator /Reagent
  • Substrate
  • Enzyme
  • Appearance if positive
  • Appearance if negative
A
  • urease media
  • phenol red
  • urea
  • urease
  • pink
  • orange
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6
Q

Nitrate Reduction

  • Test
  • Media
  • pH Indicator /Reagent
  • Substrate
  • Enzyme
  • Appearance if positive
  • Appearance if negative
A
  • nitrate media
  • Sulfanilic Acid, a-napthylamine & zinc powder
  • nitrate
  • nitrate reductase
  • red after addition of first 2 reagents. Colorless after addition of 3rd reagent
  • Colorless after addition of 1st 2 reagents. Red after addition of 3rd reagent
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7
Q

Decarboxylase

  • Test
  • Media
  • pH Indicator /Reagent
  • Substrate
  • Enzyme
  • Appearance if positive
  • Appearance if negative
A
  • lysine decarboxylase media
  • bromcresol purple
  • lysine
  • decarboxylase
  • purple
  • yellow
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8
Q

PA Deaminase

  • Test
  • Media
  • pH Indicator /Reagent
  • Substrate
  • Enzyme
  • Appearance if positive
  • Appearance if negative
A
  • PA Media
  • 12% Ferric chloride
  • phenylalanine
  • PA deaminase
  • green
  • no color change
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9
Q

Methyl Red

  • Test
  • Media
  • pH Indicator /Reagent
  • Substrate
  • Enzyme
  • Appearance if positive
  • Appearance if negative
A
  • MR-VP broth
  • methyl red
  • Glucose
  • n/a
  • red
  • yellow/orange
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10
Q

Vogues Proskauer

  • Test
  • Media
  • pH Indicator /Reagent
  • Substrate
  • Enzyme
  • Appearance if positive
  • Appearance if negative
A
  • MR-VP broth
  • VP-A and VP-B
  • Glucose
  • n/a
  • red
  • no color change/copper red
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11
Q

Indole production

  • Test
  • Media
  • pH Indicator /Reagent
  • Substrate
  • Enzyme
  • Appearance if positive
  • Appearance if negative
A
  • SIM media
  • Kovac’s Reagent
  • Tryptophan
  • tryptophanase
  • Red ring on top
  • no color change
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12
Q

Citrate Utilization

  • Test
  • Media
  • pH Indicator /Reagent
  • Substrate
  • Enzyme
  • Appearance if positive
  • Appearance if negative
A
  • citrate media
  • bromothymol blue
  • Citrate
  • citrate permease
  • blue
  • green
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13
Q

EMB

  • Test
  • Media
  • pH Indicator /Reagent
  • Substrate
  • Enzyme
  • Appearance if positive
  • Appearance if negative
A
  • EMB plate
  • Eosin Y and methylene blue
  • lactose/sucrose
  • n/a
  • pink (slow fermenters) green (fast fermenters)
  • no growth or pale yellow growth
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Microbiology summer 2015 Lab practical (4 decks)

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