biochem exam 1 Flashcards
G=H-TS
- g represents free energy change
- h is the change in enthalpy
- s is the change in entropy or randomness
dipole
between uncharged molecules
van der Waals
- determines steric complementarity
- stabilize macromolecules
f= (Q1Q2)/Er^2
force of ionic interaction depends on magnitude of charges, distance between charged groups and dielectric constant.
colligative property
- bpt, mpt, osmolarity
- don’t depend on nature of solute concentration
non colligative property
- viscosity, surface tension, taste, color
- depend on chemical nature of solute
chromatography
protein separation which protein remains fully folded
column chromatography
-separation using liquid phase
- proteins with Lowe affinity for solid wash off first
cation exchange
thing that binds column is cation and gets exchanged making column negative
size exclusion chromatography
big thing comes off first
affinity chromatography
protein with no affinity falls through column
SDS page
- separates proteins by molecular weight
- small at top large at bottom
ednan sequencing
identify amino terminal residue of polypeptide
ESI-MS
Identify peptides based on mass and charge
alpha helix
stabilized by hydrogen bonds between nearby residues
