Biochem Day 1

Question 1

Alanine aminotransferase in serum diagnosis

Answer 1

Viral Hepatitis

Question 2

Aspartate aminotransferase diagnosis in serum

Answer 2

Alcoholic hepatitis

Myocardial infarction

Question 3

Alkaline Phosphatase serum marker diagnosis

Answer 3

Osteoblastic bone diseases (Pagets, prostate cancer)

Obstructive liver disease

Question 4

Amylase serum diagnosis

Answer 4

Mumps

Acute pancreatitis

Question 5

Creatine kinase serum diagnosis

Answer 5

Myocardial infarction

Question 6

Lactate DH serum diagnosis

Answer 6

Myocardial infarction

Question 7

What rxn does carbonic anhydrase catalyze

Answer 7

CO2 + H2O= HCO3- + H+

Fastest known enzyme

Also speeds up formation of carbonic acid

Question 8

Where is carbonic acid found

Answer 8

RBC

Kidney (Reabsorption of bicarbonate at proximal tubule)

Question 9

How are zymogens activated

Answer 9

Removal of peptide fragment

Question 10

How are pancreatic zymogens release and activation mediated

Answer 10

Mediated by cholecystokinin, secretin, parasympathetic NS

Question 11

Stomach ztmogens

Answer 11

Pepsinogen

Question 12

Pancreas zymogens 5

Answer 12

• Chymotrypsinogen
• Trypsinogen
• Procarboxypeptidase A
• Procarboxypeptidase B
• Preelastase

Question 13

Von Gierkes disease deficiency in

Answer 13

Deficient in Glucose-6-phosphatase

Needed to convert glucose-6-phosphate==> Glucose

Question 14

Gluconeogenesis function and site

Answer 14

Glucose is made from carbohydrates (primarily amino acids, NOT FA)

Occurs in liver

Provides glucose to other tissues when glucose is low

Often lactic acid or AA are converted to pyruvate or phosphoenolpyruvate to be made into glucose

Question 15

Energy for Gluconeogenesis comes from

Answer 15

Fatty acid oxidation

Question 16

What type of enzyme inhibition can be overcome

Answer 16

Competitive inhibition

Noncompetitive cannot be overcome( includes allosteric inhibitors) can bind free enzymes and ES complexes

Question 17

What does a uncompetitive inhibitor bind to

Answer 17

Binds to an enzyme that already has a substrate bound

Question 18

Aspirin is an example of what type of inhbitor

Answer 18

Irreversible inhibitor

Destroy functional group

Aspirin (acetylsalicylate) irreversibly inhibits cyclooxygenase by acetylating the active site serine