Biochem Basics + Carbohydrates Flashcards
Components of Energy Expenditure
- basal metabolic rate (75% in sedentary person)
- thermic effect of food (8%)
- physical activity energy expenditure (30-40%)
Hierarchy of Fuels
- alcohol: 7kcal/g, no storage pool
- protein: 4 kcal/gram, no true storage pool
- glucose: 4kcal/gram, storage as glycogen in liver and muscle (muscle glycogen cannot be released as glucose)
- fat: 9kcal/g, large storage pool
Most Accurate Way of Measuring Total Energy Expendature
- doubly labeled water
- if a person’s weight is stable, then EI=TEE
- TEE= 25-35kcal/kg/day
- for 70kg person, EI should be about 2100 kcal/day
- this means a measure of TEE accurately predicts energy intake if weight is stable
- note: carbs= 4 kcal/g
Body Energy Stores
-fat: greatest amount (120,000 kcal)
-carbs: 2,000 kcal (mostly in form of glycogen in muscle and liver)
-
Most Accurate Way to Measure Body Composition
-DEXA scan
Counter Regulatory Hormones
- glucagon
- cortisol
- catecholamines
- growth hormone
Glycolysis
- carbohydrate pathway
- when: excess glucose in blood (after meal)
- where: cytoplasm/cytosol
- what: results in breakdown of 6 carbon glucose to 2 pyruvate and 2 ATP
- who: glucose-> pyruvate, if O2 not present or no mitochondria, pyrvate -> lactate
Tricarboxylic Acid Cycle (TCA Cycle)
- carbohydrate pathway
- what: conversion of pyruvate to NADH, FADH2, and GTP
- where: in cell
- when: when oxygen and mitochondira are present
- who: pyruvate-> CO2, GTP (ATP), NADH, and FADH2
- in mitochondria matrix
Electron Transport
- carbohydrate pathway
- where: mitochondrial membrane
- who: NADH and FADH2 -> ATP from ADP, O2 is consumed and H2O is produced in oxidative phosphorylation
- in mitochrondria
Gluconeogenesis
- carbohydrate pathway
- when: in state of negative energy balance
- what: break down of carbon skeletons -> glucose, new glucose production
Glycogen
- carbohydrate pathway
- what: storage of excess glucose, immediately available in low energy states, highly brached polymer of glucose
- where: most glycogen is stored in the liver and skeletal muscle
Pentose Phosphate Pathway (Hexose Monophosphate Shunt)
- carbohydrate pathway
- what: activated when glucose is present in excess or need for pathway products
- who: generates NADPH for biosynthesis of fatty acids and steroids and ribose for synthesis of nucleotides
- where: mammary gland, adrenal cortex, liver, adipose tissues, in cytosol
NADPH
- created in pentose phosphate pathway (HMS)
- provides energy for synthesis of fatty acids and steroid hormones
Ribose
- formed in pentose phosphate pathway (HMS)
- used as building block in synthesis of RNA and DNA
Triacylglycerol Synthesis (De Novo Lipogenesis)
- fat pathway
- who: glucose + acetyl coA -> fatty acids
- what: glucose conversion to fat
Beta Oxidation
- fat pathway
- fatty acids are taken up by tissues such as liver and muscle where they are catabolized two carbons at a time in a process called beta oxidation
Ketogenesis
- fat pathway
- production of ketone bodies from fat
- produced when insulin is very low ans counter-regulatory hormones are very high
Triglycerol Degredation
- fat pathway
- when: in negative energy balance
- what: stored fat from adipose tissue can provide energy to oxidizing tissues such as muscle and liver as an alternative to glucose
Urea Cycle
- protein pathway
- pathway involved in disposal of nitrogen derived from the metabolism of amino acids
- urea enters blood as blood urea nitrogen (BUN)
- exreted by kidneys
Hexokinase
- enzyme that phosphorylates glucose to glucose-6-phosphate
- key step in glycolysis: activation of glucose, first ATP investment
- present in most tissues except liver and pancreatic beta cells
- at low [glucose], hexokinase sequesters glucose in the tissue
- inhibited by glucose-6-p
- irreversible rxn
- not very selective
- present in all cells
- low Km for sugars
Glucokinase
- enzyme that phosphorylates glucose to glucose-6-phosphate
- key step in glycolysis: activation of glucose, first ATP investment
- present in liver, beta cells of pancreas
- at high [glucose] excess glucose is stored in liver
- higher Vmax and Km than hexokinase
- inhibited by fructose-6-p
- irreversible rxn
- selective for glucose
- high Km for glucose
Km
-[substrate] at which rxn is half maximal
Phosphofructokinase-1
- enzyme that phosphorylates fructose-6-phosphate to fructose 1, 6-bisphosphate
- part of glycolysis
- rate limiting step
- activated by AMP, fructose-2, 6-bisphosphate
- inhibited by ATP, citrate
Function of Kinase
-uses ATP to phosphorylate a substrate
Function of Phosphatase
-removes phosphate group from substrate
Phosphofructokinase-2 (PFK-2)
- dephosphorylated PFK-2 is active
- active PFK-2 favors formation of fructose 2, 6-bisphosphate -> inc. PFK-1-> inc. glycolysis
Pyruvate Kinase
- enzyme that converts phosphoenolpyruvate (PEP) to pyruvate
- key step of glycolysis
- activated by fructose-1, 6-bisphosphate
- inhibited by ATP, alanine, phosphorylated (inhibited) by PKA
- irreversible rxn
Lactate Dehydrogenase
- facilitates interconversion of pyruvate and lactate
- driven by [substrates]
Pyruvate Dehydrogenase
- key step in TCA cycle
- mitochondrial enzyme complex linking glycolysis and TCA cycle
- active in fed state (inc. insulin)
- rxn: NAD+ + CoA + pyruvate -> acetyl CoA + CO2 + NADH
GTP
-substrate level phosphorylation in TCA cycle
Products of TCA Cycle
- 3 NADH
- 1 FADH
Citrate
- converts acetyl CoA and oxaloacetate to citrate
- TCA cycle
a-Ketogluterate DehydrogenasE
- converts a-ketogluterate to succinyl coA
- TCA cycle
Succinate Dehydrongenase
- converts succinate to fumarate
- TCA cycle
Malate Dehydrogenase
- converts malate to oxaloacetate
- TCA cycle
Electron Transport Chain
- NADH (and FADH) electrons from glycolysis enter mitochondria
- passage of e- results in formation of proton gradient that couples with oxidative phorphorylation to drive production of ATP
Glut 4
- allows glucose to enter cell and begin glycolysis
- transporter
- insulin sensitive (inc. glucose transport in response to insulin)
- skeletal muscle, adipose tissue
Glut 2
- glucose transporter
- liver
- not insulin sensitive
Substrate Level Phosphorylation
-phorphorylation of ADP to ATP by high energy phosphate compounds formed during glycolysis
Glucose 6 Phosphatase
- present in liver and kidney
- converts glucose 6 phosphateto glucose by removing a phosphate
- gluconeogenesis
Fructose 1-6 BisPhosphatase
- converts fructose 1, 6 bisphosphate to fructose 6 phosphate
- gluconeogenesis
- deficiency: late hypoglycemia following fasting, severe lactic acidosis, inc. pyruvate, inc. ketones
PEP Carboxykinase
- converts pyruvate to oxaloacetate
- gluconeogenesis
Pyruvate Carboxylase
- converts pyruvate to oxaloacetate
- gluconeogenesis
Hemolytic Anemia Enzyme Deficiency
- G6PD deficiency
- pyruvate kinase deficiency
Glucagon
- causes phosphorylation
- synthesized in pancreatic a cells
- action: interacts with G protein that inc. intracellular levels of cAMP
- causes inc. in gluconeogenesis and glycogenolysis
- inc. liver glucose production
- reciprocally regulated by insulin and vice cersa
- also promotes lipolysis and ketogenesis
- regulation: secreted in response to hypoglycemia and inhibited by hyperglycemia
- degraded in liver, half life of 5 min
Insulin
- causes dephosphorylation
- intermediates in metabolic pathway: PI3K, AKT
- intermediates in mitogenic pathway: MAP kinase
- derived from pro-insulin by cleavage of connecting peptide (C peptide)
- exposure of islet cells to inc. glucose results in surge of insulin followed by decline and then rise as long as glucose remains high
- initiators: glucose, amino acids, sulfonylurea drugs (glyburide)
- inhibitors: diazoxide, somatostatin, a adrenergic agents, glucose toxicity
- inc. glucose -> inc. ATP-> intracellular signal-> inc Ca-> exocytosis of insulin
- inc. insulin/dec. catecholamines inhibit lipolysis
- very low insulin -> ketogenesis
Pyruvate Dehydrogenase Complex
- in mitochondrial matrix
- converts pyruvate to acetyl coA in aerobic, fed conditions
- inhibited by acetyl coA, ATP, fatty acids, and NADH
- inactive in phosphorylated state
- active in dephosphorylated state
- activated by AMP, CoA, and NAD+
- thiamine (vit B1) deficiency: inability to oxidize pyruvate-> wernicke encephalopathy
- TCA cycle
Citrate
- citrate synthase converts acetyl coA and OAA to citrate
- irreversible rxn
- TCA cycle
- where fatty acid synthesis takes off
a-Ketogluterate
- isocitrate dehydrogenase converts isocitrate to a-ketogluterate
- produces CO2and NADH
- TCA cycle
- important entrance point for amino acids that contribute to gluconeogenesis
Succinyl CoA
- a-ketogluterate dehydrogenase converts a-ketogluterate to succinyl coA
- CO2 and NADH are made
- TCA cycle
- important entrance point for amino acids and fatty acid products
GTP
-formed as succinyl coA is converted to succinate in the TCA cycle
Fumarate
- converted from succinate by succinate dehydrogenase
- TCA cycle
- FADH2
- entrance point for amino acids
- byproduct of urea cycle
Malate
- converted from fumarate by fumarase
- TCA cycle
- also involved in gluconeogenesis
Oxaloacetate
- converted from malate by malate dehydrogenase
- generates NADH
- involved in gluconeogenesis
UDP-Glucose
-activated form of glucose used in glycogen synthesis
Glycogen Synthase
- catalyzes the transfer of glucose from UDP glucose to grown glycogen chain
- forms an a-1,4 glycosidic linkage
- key regulated enzyme in glycogen synthesis
- glycogenin is enzyme that forms initiating site for glycogen synthesis
- activated by G6P in fed state
Branching Enzyme
- forms a-1,6 linkages in glycogen synthesis
- branching inc. solubility of glycogen
- branching inc. rate of glycogen synthesis and degredation
- deficiency: abnormal non-branched glycogen acculumates in the liver and skeletal muscle
- hypoglycemia not a prominent sx
Glycogen Phosphorylase
- catalyzes cleavage of glycogen to G-1-P
- key regulated enzyme in glycolysis
- inhibited by G6P in fed state
- activated in exercise by inc. Ca binding to calmodulin-> activation of phosphorylase kinase -> phosphorylates glycogen phosphorylase (activation)-> glycogen degredation
- activated by AMP in muscle during exercise
- deficiency: hepatomegally, short stature, mild muscle weakness
- tx: raw cornstarch
Debranching Enzyme
- converts the branched glycogen structure into a linear one, which allows for further cleavage by glycogen posphorylase
- accumulation of abnormal glycogen in liver and muscle
Glycogen Synthesis and Breakdown Regulation
- hormonal: insulin and glucagon
- allosterically
- regulation in fed state: glycogen synthase allosterically activated by G6P, glycogen phosphorylase is allosterically inhibited by G6P
Glucose-6-Phosphate Dehydrogense (G6PD)
- catalyzes first rxn in pentose phosphate pathway
- rate limiting step
- generates first NADPH
G6PD Deficiency
- severe fasting hypoglycemia occurring within 3-4 hours after a meal
- certain compounds such as sulfa antibiotics, antimalarial drugs, and fava beans react with GSH and deplete it
- leads to RBC rigidity -> destruction and hemolytic anemia
- most severe glycogen storage disease
- enlarged liver
- the deficient enzyme is responsible for producing free glucose to leave the liver
- tx: constant supply of oral glucose
Pancreatic B Cell
- secrete insulin
- arranged in central core
Pancreatic A Cell
- secrete glucagon
- these cells sit next to insulin secreting cells
Key Intermediates for Metabolic Insulin Pathway
-PI3K and AKT
Key Intermediates for Mitogenic Insulin Pathway
-MAPK
Insulin Resistance
- action of insulin is reduced in peripheral tissues
- initially beta cells secrete more insulin
- becomes type 2 diabetes
- generally NOT caused by issues with insulin receptor
- caused by abnormal phosphorylation of serine/threonine
Incretin Effect
- oral glucose causes much more insulin secretion that when glucose is infused via IV
- caused by GLP-1 and GIP
GLP-1
- production: product of glucagon gene expressed in pancreatic alpha cells and L cells of intestinal mucosa
- secretion: stimulated by presence of nutrients in gut
- actions: potent insulin releasing substance, inhibits glucagon secretion, inhibits GI motility, inhibits appetite
- degredation: short half life, broken down by DPP-4
Catecholamines
- norepi and epi inc. blood glucose by inc cAMP
- elevated in stress
Corticosteroids
- cortisol is secreted in response to stress
- results in inc. blood glucose
- slow time course
- chronic exposure associated with development diabetes
Growth Hormone
- produced by anterior pituitary gland
- concentration inc. due to hypoglycemia and stress
- tends to dec. insulin sensitivity
Somatostatin
- inhibits growth hormone
- inhibitor of insulin and glucagon
- inhibits gut motility, splanchnic blood flow and secretion of digestive enzymes
Oligosaccharides
- 3-9 sugars
- present in beans, onions, vegies
Starches
- long polymers of glucose
- amylopectin: highly branches
- amylose: unbranched
- resistant starch: corn starch, slowly absorbed
Glycemic Index
- ranks carbs based on their rate of glycemic response (conversion to glucose within the human body).
- uses a scale of 0 to 100, with higher values given to foods that cause the most rapid rise in blood sugar
- low GI 70
Glycemic Load
-glycemic index x amount of food eaten
Fructose Metabolism
- bypasses PFK in glycolysis pathway and result is that it is rapidly driven down glycolysis and less regulated than glucose
- intolerance: hypoglycemia, sx follow introduction of fructose, N/V,
Fibers
-complex carb that is not digestible by humans
Types of Studies That Can Inform Nutritional Decisions
- animal studies
- epidemiological studies
- small randomized
- large randomized: gold standard
Lactate
- byproduct of glycolysis
- very important carb source
Galactosemia
- deficiency in enzyme that produces UDP galactose: galactose-1-phosphate uridyltransferase (GALT)
- jaundice, cataracts, N/V
- tx: restrict lactose