Biochem 6 (Laboratory Techniques)

Question 1

Describe the steps involved in PCR

Answer 1

1) Denaturation by heat
2) Annealing of pre-made DNA primers
3) Elongation of primer by hsDNA polymerase

Question 2

What are the properties of a DNA sequence that would travel the furthest on electrophoresis?

Answer 2

Small and high negative charge

Question 3

Describe the steps of a southern blot.

Answer 3

1) DNA sample
2) DNA probe
3) Viewed on radiography

Question 4

Describe steps of northern blot

Answer 4

1) RNA sample
2) DNA probe
3) Radiography viewing

Question 5

Describe the steps of a Southwestern Blot (Used to identify transcription factors)

Answer 5

1) DNA Binding protein sample
2) Oligonucleotide probe
3) Radiography viewing

Question 6

What is the confirmatory test following a positive ELISA?

Answer 6

Western Blot

Question 7

For Knock-in mice, what are the two ways by which you can insert the gene of interest?

Answer 7

Random insertion = Constitutive

Targeted insertion = Conditional

Question 8

Describe the steps of a Western blot

Answer 8

1) Protein sample
2) Ab probe
3) Radiography viewing