bio microscope test Flashcards

1
Q

how do you carry a microscope

A

in an up right position, one hand under the base, and the other hand on the arm

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2
Q

what are the 5 steps to putting away a microscope

A

1- turn it off
2- the stage is all the way up
3- place on the scanning objective (the red one and the smallest)
4- eye piece even with the arm
5- clean microscope with an alcohol wipe but not the lenses

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3
Q

how do you clean a microscope slide

A

you can only use lens paper while cleaning it

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4
Q

what are the steps to making a temporary slide

A

1- put the object on glass slide and make sure its small enough to fit under the cover slip
2- drop water on the object
3- with forceps (tweezers) place the cover slip over the object
(if there are large water bubbles drop water right next to the cover slip)

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5
Q

how do you stain a slide

A

1- make a temporary slide and the cover slip MUST be flat
2- drop lodine on one side of the cover slip
3- use the paper towel square on the other side to pull it through
(you can clean this uses lens paper)

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6
Q

how do you find an object on the low power objective

A

1- adjust the diaphragm
2- bring the stage all the way up
3- move the course adjustment slowly till the object comes into focus
4- use the fine adjustment to bring out detail and make it cleaner

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7
Q

how do you fins an object on the high power objective

A

1- find it on low power first
2- without moving the stage turn to high power
3- center the object
4- use the fine adjustment only to bring out the detail
never use the course adjustment

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8
Q

identify all parts of the microscope

A

look at notes (no word bank)

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9
Q

explain the characteristics of a compound light microscope (a-e)

A

A- use light to form on image
B- provides a flat image
C- can be used on living and non-living things
D- the higher the magnification, the worse the resolution
E- highest magnification is 2000x

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10
Q

What are the advantages and disadvantages of a compound light microscope

A

advantages - can be used on living and non-living things
disadvantages - the higher the magnification, the worse the resolution

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11
Q

what type of microscope is used in class

A

compound light microscope

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12
Q

define resolution

A

the clarity or sharpness of the image

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13
Q

define image

A

what you see under the microscope

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14
Q

define iris diaphragm

A

has a central opening with over lapping plates that can be adjusted to increase or decrease the control opening

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15
Q

define disc diaphragm

A

consists of a flat plate, with various sized holes

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16
Q

define ocular lens

A

another name for the eye peice

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17
Q

define optical microscope

A

Any microscope that used light to form an image

18
Q

define examples of optical microscope

A

microscope, magnify glass, camera, binoculars, telescope, and glasses

19
Q

define stereomicroscope

A

used to view external structures and used in direction. Provides a 3D image. The magnification is 10x - 60x. The advantages is that it provides the 3D image. The disadvantage is that it only has 10x - 60x

20
Q

define total magnification

A

found by multiplying the eyepiece times the objective (low - 10 / high - 40)

21
Q

what are the 5 characteristics of an electron microscope

A

1- it uses electrons to form an image
2- it can only be used on non-living things
3- some will provide a flat image (transmission), and some a 3D image(scanning)
4- the higher the magnification, the better the resolution
5- it can magnify up to 2,000,000x

22
Q

what color is the high power objective

A

blue

23
Q

what is the magnification of the high power objective

A

40x

24
Q

what color is the low power objective

A

yellow

25
Q

what magnification is the low power objective

A

10x

26
Q

what color is the scanning objective

A

red

27
Q

what is the magnification of the scanning objective

A

4x

28
Q

what is the function of the eyepiece

A

what you look through to see object

29
Q

what is the function of the arm and base

A

carry the microscope

30
Q

what is the function of the fine adjustment

A

brings image into focus slowly

31
Q

what is the function of the course adjustment

A

brings object into focus quickly

32
Q

what is the function of the lamp or mirror

A

directs light into the microscope

33
Q
A
34
Q

what is the function of the iris or disc diaphragm

A

regulates amount of light

35
Q

what is the function of the iris lever

A

increase / decrease light coming through

36
Q

what is the function of the stage opening

A

lets light through

37
Q

what is the function of the stage

A

holds the slide

38
Q

what is the function of the stage clips

A

holds the slide in place

39
Q

what is the function of the low power objective

A

magnifies on image 10x

40
Q

what is the function of the high power objective

A

magnifies on image 40x

41
Q

what is the function of the revolving nose piece

A

changed objectives