Bio Lab Practical Flashcards
Arm:
Supports and connects the eyepiece lens to the base, Microscope should be held here.
Base
Supports the Microscope
Coarse Focus Knob
Used to move stage up and down (Using with scanning objective)
Condenser
Used to focus light on Specimen (Found Beneath the stage )
Eyepiece/Ocular Lens
Ocular lens 10x magnification
Fine Focus Knob
Used to adjust the specimen into final focus
Light source
Serves as source of light for the microscope
Scanning Objective
Shortest Objective , Magnifies 4x , ALWAYS START FIRST
Low Power Objective
Used for coarse and preliminary focusing magnifies 10x
High Power Objective
Used for final focusing , Magnifies 40x
Stage
Platforms on Which slides are placed , Knobs can move stage up down or side to side
Stage Clips
Secures the slides
Monocular Microscope
A microscope with one ocular
Binocular Microscope
A microscope with two oculars
Resolution is…
The smallest distance between two points on a specimen that can still be distinguished as two separate entities
Magnification is..
Getting closer to the specimen/magnifying the view. This indicates how many times the object you see has been enlarged
Protein=
Biuret Reagent
Simple sugars=
Benedict solution
Starch=
Lugols Solution
Positive control is
Uses a treatment that is known to influence the results(achieves results similar to hypothesis)
Negative Control is
Uses a treatment that is know NOT to influence the results (given a placebo or no treatment at all)
What does Gel electrophoresis do?
It separates the DNA and makes it easier to analyze based on size and charge.
What are micropipettes used for?
to transfer volumes of samples into the well in the gel
what is Electrophoresis?
a technique used to separate dna,rna or protein based on their size and electrical charge. The electric current is used to make molecules move throughout the gel
What is the outcome of using Electrophoresis. gel?
we get to see how many different dna fragments are present in a sample and how large they are relative to one another
What does Size Exclusion Chromatography do?
Separates molecules by filtration through a gel
What does paper chromatography do
The solvent carries the dissolved pigment as it moves up the paper
What happens in Strawberry lab
the alcohol pushes the dna out of the liquid while the rest of the liquid remained in the solution. there was a white/gooey clear dna strands in the alcohol layer and the 2 layers. the dna clumped in this activity making it easier for us to see the single strands of dna
Dihybrid Cross is
a cross between 2 organisms with both being heterozygous for 2 different traits
Pedigree is
a chart that diagrams the inheritance of a trait or condition through generations of a family
what is the General importance of model organisms?
it provides valuable insight into biological systems at the cell, tissue, organ or system level. BRASSICA RAPA= they do not self pollinate, they are genetically diverse . it can be informative in research of disease
In cold water , the food coloring
spreads out throughout the water SLOWLY
In hot water , the food coloring
spreads out and diffuses faster . at high temperatures particles move faster. This faster movement allows diffusion to occur more quickly
Chemical changes to strawberry
Ethanol-repeated the dna
salt-draws out the liquid
Dialysis Tubing
Starch Solution in the tube turned colors because the lugols solution was not able to pass through . The starch molecules are too big to exit through the tube
