BIO LAB MID TERM Flashcards

1
Q

What are the lab safety policies?

A
  • Let the TA know about any injuries,chemical spills, broken glass and fire
  • No eating or drinking
  • Before leaving lab; wash hands, clean table and place materials in their original place
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2
Q

What are the pieces of safety equipment?

A
  • Gloves (left/back side of the lab)
  • First Aid Kit (front part of the lab)
  • Fume Hood (left/front part of the lab)
  • Glass Disposal (left/front part of the lab)
  • Exit Sign
  • Eye Wash Station (right/front part of the lab)
  • Hazardous Waste Dispoal (Left/front part of the lab)
  • Fire Extinguisher
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3
Q

What are the lab dress codes?

A
  • Close-toed shoes with enclosed heel
  • Top must cover midriff, back and shoulders
  • Pants (no shorts or skirts)
  • No mesh clothing or holes
  • Hair tied back
  • Googles and lab coats NOT required
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4
Q

Syllabus/Course Policies Overview

A
  • Attendance is required, any absences due to illness, emergencies, religious holiday, athletic events or military must have an absence form submitted prior.
  • AI Use is prohibitted
  • Labs must be made up by either scheduling it in another section or attending an in genernal lab near the end of the semester
  • Must email instructor with only rebelmail accounts
  • Quizzes are worth 20% (lowest dropped)
  • Pre-Labe Quizes are worth 15% (lowest dropped)
  • Lab Report are worth 20% (lowest dropped)
  • PLPs are worth 5%
  • ESB are worth 15%
  • Midterm worth 10%
  • Final worth 15%
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5
Q

What equipment is used to measure things like mass,length,volume, temperature?

A
  • Beaker
  • Flask
  • Graduated Cylinder
  • Test Tube
  • Transfer Pippette
  • Electric Balance
  • Thermometer
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6
Q

What are units of measurement for volume,mass,length and temperature?

A
  • Volume: militers or liters
  • Mass: grams or kilograms
  • Length: milimeters,centimeter,inches, feet,yards, meters,kilometer, miles
  • Temperature: celsius, fahrenheit, kelvin
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7
Q

What are the metric base units of measurement?

A
  • Deci (d): 0.1 (10^-1)
  • Centi (c): 0.01 (10^-2)
  • Mili (m): 0.001 (10^-3)
  • Micro (upside-down h): 0.000001 (10^-6)
  • ## Nano (n): 0.0000000001(10^-9)
  • Deka (da): 10 (10^1)
  • Hecto (h): 100 (10^2)
  • Kilo (k): 1000 (10^3)
  • Mega (M): 1,000,000 (10 ^6)
  • Giga (g): 1,000,000,000 (10^ 9)
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8
Q

Converting from metric to english

A

1 milimeter —-> 0.039 inches
1 centimeter —-> 0.4 inches
1 meter —-> 3.3 feet
1 kilometer —–> 3.3 miles
1miligram —-> 0.001 gram
1 gram—–> 0.0353 ounces
1 kilogram —–> 2.2 pounds

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9
Q

G

Converting to and from scientific notation

A

Converting to scientific notation
* 52,314 —-> 5.2314 x 10^4
* 0.000056—–> 5.6 x 10^5

Scientific notation to standard
* 4.5 x 10^4 ——> 45,000
* 500—-> 5 x 10^2

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10
Q

What are the steps of the Scientific Method?

A
  1. Observation
  2. Hypothesis
  3. Experiment
  4. Data Collection/Analysis
  5. Communication
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11
Q

What are dependent, independent, and control variables?

A

Dependent: The component that is being measures (depends & will change based off the independent variable)

Independent variable: The component that we are manipulating/changing (will effect the dependent variable results)

Control variable: Represents a normal/typical scenario, produces a known result that will used as the standard for IV (usually the placebo)

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12
Q

On a graph what is the dependent and independent variable?

A

Dependent Variable: Y axis
Independent Variable: X axis

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13
Q

What two things will water ionize/dissociate into?

A

hydrogen ion (H+) and hydroxl ion (OH-)

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14
Q

What is an acid?

A

Any compound that releases hydrogen ions when dissolved in water

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15
Q

What is a base?

A

A compound that accepts hydrogen ions while in solution removing free hydrogen ions form solution

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16
Q

What is the pH scale?

A

A scale with various ranges of pHs, from 0 being the most acidic to 14 being the most basic and 7 being neutral

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17
Q

How does the pH scale function?

A

Based off the powers of ten

Acidic: positive expontents; 10^5=100,000 (H+)

Basic: negative expontents: 10^-4= 1/1,000 (OH-)

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18
Q

Acidic pH examples

A
  • lemon
  • stomach acid
  • battery
  • tomato
  • vinegar
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19
Q

Basic pH examples

A
  • baking soda
  • soap
  • bleach
  • drain cleaner
  • ammonia solution
20
Q

What are atoms, atomic number, atomic mass?

A

atom: basic unit of chemical
atomic number: number of protons in the nucleus
atomic mass: number of protons plus number of neutron

21
Q

What is a chemical bond?

A

Interaction between atoms

22
Q

What is an Ionic, Covalent, Non-polar, and Polar bond?

A

Ionic: Attraction between oppositely chagred ions (loss or gain of electrons)
Covalent: Sharing of electrons
Nonpolar: Equal sharing of electrons
Polar: Unequal sharing of electrons

23
Q

Why are positive and negative controls used?

A
  • Ensure procedures are working correctly
  • Detect sample contamination
  • Used as a reference point for comparison of results
24
Q

What is a carbohydrate and what are monosacchaarides, disaccharides, oligosaccharides
and polysaccharides?

A

Carbohydrate: Molecule containing carbon, hydrogen and oxygen and plays a key role in an organism’s energy production/celluar make up

Monosaccharide: Single, simple sugar (monomer); building blocks for larger molecules

Disaccharide: Two monosaccharides linked

Oligosaccharide: Three to seven monosaccharides

Polysaccharides: Complex carbohydrates, 12+ monosaccharides linkes; serve as energy storage molecules

25
What is the benedict's test?
A test to detect reducing sugars (monosaccharides and some disaccharides)
26
What are the color results in the Benedict's test and their respective meaning?
Blue: Negative Green: Positive Orange: Positive Red: Positive
27
What is Lugol's Iondine test?
Test used to detect the presence of starch
28
What are the color results in the Iodine test and their respective meanings?
Yellow: Negative Purple: Postive
29
What are the four major marcomolecules?
* Carbohydrates * Protein * Lipids * Nucleic Acid
30
What is the Biuret test?
Test used to detect the presence of proteins
31
What are the color results for the Biuret test and their respective meanings?
Purple: Positive Blue: Negative
32
What is the Ninhydrin test?
Test used to detect the presence of amino acids
33
What are the color results for the Ninhydrin test and their respective meanings?
Purple: Postive Clear: Negative
34
What is the Sudan III Test?
Test to detect the presence of lipids
35
What are the results for the Sudan III test and their respective meanings?
Two Layer (orange/red): Postive One full layer (orange/red): Negative
36
Differences between DNA and RNA
DNA * Double stranded * Contains deoxyribose as 5 carbon sugar * thyime RNA * Single stranded * Contains ribose as 5 carbon sugar * Uracil
37
What is the Dische Diphenlamie Test?
Test to detect the presence of DNA
38
What are the color results for the Dische Diphenylamine test and their respective meanings?
Blue: Postive Clear: Negative
39
What is the ocular portion of the microscope?
It's the part in which you use to look through and view the specimen
40
What is the noise piece of a microscope?
Structure that holds the objective lenses
41
What is the arm portion of the microscope?
A major support structure and should always have one hand on when moving the microscope
42
What are the four objective lenses?
Scanning objective (4x): Smallest objective, used before focusing on higher powers Low-power objective (10x): Higher objective than 4x High-power objective (40x): Long enough to touch stage if not careful and only the fine focus knob should be used when on this objective Oil immersion objective (100x): Highest objective, requires a drop of oil in order to help the light focus on the slide
43
What is the iris diaphragm of the microscope?
Dial found below the front of the stage; used to control the amount of light that passes through the consender.
44
What is a parfocal on a microscope?
It allows the microscope to stay in focus when changing between lenses
45
How do you calculate total magnification?
Objective magnification x Ocular magnification 4x x 10x = 40x
46
What were the three (prokaryotic) bacteria shapes that were viewed under the microscope?
Coccus: Spherical Bacillus: Rod Spirillum: Spiral
47
What was the eukaryotic cell that was viewed under the microscope?
Paramecium