BIO Ch1 microscopes Flashcards
who came up with the idea of cells? (and added lens and light to the microscope)
Hooke (1665)
who was the first to observe the movement of living cells?
Van Leeuwenhoek (1674)
who came up with the idea that all living things are made of cells, and that cells are a basic unit of life?
Schleiden & Schwann (1830’s)
who came up with the idea that new cells come from pre-existing ones?
Virchow (1885)
Describe a light microscope
Light passes thru specimen
(adv) cheap, small easy
(disadv) low mag/res, hard to view live specs
1000x
Describe a confocal microscope
Uses computers and lasers to focus light on a small area
(adv) high res, 3D images, live specs
(disadv) low mag, $$$, big
1000x
Describe a (SEM) Scanning Electron Microscope
Beam of electrons is reflected off the specimen
(adv) surface, 3D images, high mag
(disadv) $$$, big, no live specs, b&w
1,000,000x
Describe a (TEM) Transmission Electron Microscope
Beam of electrons passes through specimen
(adv) interior, highest mag
(disadv) $$$, big, no live specs, b&w
50,000,000x
what is the magnification calculation?
ocular lens x objective lens
what is the field of view calculation?
(hpfd/lpfd) = (lp mag/hp mag)
what is the actual size calculation?
field diameter/# of objects that fit across field diameter
what is the scale calculation?
size in diagram/actual size of object
what is contrast?
different colour backgrounds in order to see depth in specimen
what is resolution?
a clear image, the ability to distinguish between structures
what is fluorescent microscopy?
a green fluorescent protein (GFP) stain is added to specimen. UV light makes stain glow. Different structures glow at different amounts
