Bergeron Proteins

Question 1

FliC

Answer 1

Flagellin. 11 stranded. Multiple monomers make up filament.

Question 2

FlgH and I

Answer 2

Form the LP ring in the Basal Body.

Question 3

FliF

Answer 3

Makes up the MS ring in the Basal Body. 24 Prot complex. First to oligomerize in assembly, recruits FliG next.

Question 4

Fli PQR

Answer 4

Exporter Apparatus. IM gate. FliP is pentamer on top of the IM, 4Q and 1R make up pentamer in the inside.

Question 5

FcpA

Answer 5

Extra sheath layer surrounding the filament in Spirochetes.

Question 6

FlhA

Answer 6

Exporter gate/Substate forming platform. 9 mer.

Question 7

FliR

Answer 7

ATPase 6 mer.

Question 8

MotA

Answer 8

Tetramer of 4 TM alpha helices. 16 per 2 MotB. Loop that binds FliG on the rotar after a conformation change.

Question 9

MotB

Answer 9

1 TM alpha and PG binding domain = Anchors it and makes it static. Key Asp32 that binds H+ to trigger conf change in MotA.

Question 10

FliGMN

Answer 10

Rotar. 34mer. G binds MotA and MN binds CHeY. G recruited by FliF firstm then MN.

Question 11

PomA and B

Answer 11

MotAB equivalents but in Vibrio. Have extra periplasmic proteins (H and T rings) to strengthen the anchoring.

Question 12

CheY

Answer 12

Gets phosphorylated to interact with FliM and N in rotar = Conf change and reverse the direction that it swims.

Question 13

FlhF

Answer 13

Diffuses to the edge and starts making Flag.

Question 14

FlhG

Answer 14

Stops F making flag if there already is one

Question 15

FlgBCFG

Answer 15

Rod Proteins

Question 16

FliE

Answer 16

Key for rod assembly, chaperone?

Question 17

FlgK and L

Answer 17

Form hook junction/cap

Question 18

FlgE

Answer 18

Hook protein

Question 19

FlhB

Answer 19

Regulates second substrate switch = Cleaves itself at a loop to change specificity one FliK dissociates

Question 20

FliK

Answer 20

Ruler prot, measures hook length.

Question 21

FliD

Answer 21

Filament cap

Question 22

MreB

Answer 22

Central regulator of cell shape and maintenance
No twist, AP, not staggered
Phobic loop to anchor it to the membrane.
Forms patches = Binds to PG synth complex, allows elongation but not increase in width

Question 23

ParM

Answer 23

Actin homologue.
Plasmid segregation. LH
DS

Question 24

alfA

Answer 24

Actin homologue. No IIB

Question 25

FtsA

Answer 25

Actin homologue.

Binds membrane and ftsZ =DS in divisome. SS alone. Ic not Ib.

Question 26

FtsZ

Answer 26

Tubulin homologue. Binds ftsA to = DS in divisome. Hydrolysis of GTP = depol = constrict membrane

Question 27

TubZ

Answer 27

Tubulin homologue.

Segresome. 4 stranded tube but mono

Question 28

Crescentin

Answer 28

Poly in bottom of caulobacter = Bean shaped

Question 29

CrvA

Answer 29

Responsible for all curvature in Vibro Cholerae

Question 30

MinD

Answer 30

Oscillates from pole to pole binding curved.

Question 31

MinC

Answer 31

Binds D and inhibits ftsZ poly = Keeps it in the middle.

Question 32

MinE

Answer 32

Depol MinD to ensure it keeps oscillating.

Question 33

TubZRC

Answer 33

Type 3 seg system. Tubulin like. Z binds R, elongates to the membrane

Question 34

ParMRC

Answer 34

Type 2 seg system. Actin like. Just duplicated, M forms from plasmid to push other one away.

Question 35

ParABS

Answer 35

Type 1 seg system. MinD like. A on DNA and binds B = depol it = Walks along chromo to find more.

Question 36

MamK

Answer 36

DS linear filament. Non- Staggered.
Non-processive.
Bind line of crysallized Fe to keep it linear.

Question 37

DnaK

Answer 37

Key gene in protein folding.

K/o = Huge inclusion bodies but cell is fine.

Question 38

Evolution model for flagella

Answer 38

• > Pore in IM = FliF
• > PQR added = Gated pore
• > F1F0 ATP synthase recruited = AT = Type III SS
• > Surface adnesion pilus, evolved by duplications to have a Cap, a Rod.
• > Protoflagellum, added L and P rings
• > Flagellum