Benedict's test for reducing sugars

Question 1

What does Benedict’s reagent test for?

Answer 1

Reducing sugars

Question 2

How do results of the Benedict’s test indicate different concentrations of reducing sugars?

Answer 2

Blue: negative
As the colors get closer to red (green-yellow-orange-red) the concentrations increase

Question 3

What are the controlled variables when using Benedict’s test to estimate reducing sugar concentration?

Answer 3

-Volume of Benedict’s reagent
-Volume of sample added
-Time left in water bath

Question 4

What does iodine solution test for?

Answer 4

Starch

Question 5

What is the observation for a positive iodine test?

Answer 5

The solution turns from brown to blue-black.

Question 6

Outline the procedure to find the concentration of an unknown reducing sugar solution.

Answer 6

Carry out Benedict’s tests for a range of standard reducing sugar solutions to produce color standards.
Carry out a Benedict’s test on the unknown solution using the same volume of solution and length of time in the water bath and compare the resulting color.