bee PQ Flashcards
Where do you see the symptoms of Ascaphaerosis larvarum?
Larvas eat the spores and they penetrate the gut wall and cuticle of the thorax. Larva becomes fluffy and swollen and covered in white cotton like mycelium. The larva in the cell resembles a chunk of chalk. Fruiting bodies can be seen on the dead larvas. Larva dies within the first 2 days after their cell has been capped.
Life of the workers
- 21 days (3 + 6 + 12)
- 1-2 days - cleaning of cells
- 3-5 days - feeding older larvae
- 6-11 days - feeding younger larvae
- 12-17 days - comb construction
- 18-21 days - gardening, defending function
- From 22nd day - foraging for pollen and nectar
Life of a queen
• 16 days (3 + 5 + 8)
• Life of a drone
• 24 days (3 + 7 + 14)
• The dance of the bee
- It’s a way bees show each other where they have found nectar and pollen. With the help of the location of the sun and the gravity they can signal to other workers where they need to go.
- Round dance and waggle dance
• What does Nosema infect?
- It’s a parasitic infection in adults (mostly workers but also queen and drones)
- It affects the epithelium of the mid-gut
• What colours does the bees see, and which do they not see?
• They can see UV light, but not red light - it looks black to them
• For how many hours can you lock the beehive?
• For maximum of 72 hours
• How long do you have to boil the comb?
• On 49 degrees C for 24 hours to get rid of all stages of wax moths.
• Which diseases belong to the OIE-B?
- Acarapiosis of bees
- American and European foul brood
- Nosemosis of bees
- Varroosis
- Asian mite: Tropilaelaps clareae, T. Mercedesae
• What is honeydew?
• It is a classification of honey that refers to honey produced by honeybees collecting nectar that is exuded from another insect such as an aphid or scale insect. Very common is the one from black forest in Germany.
• When do the bees produce the most wax?
- When they want to build and strengthen the comb and produce more cells
- Mostly between 12-17 days
• When did they first observe Varroa destructor in Hungary?
• 1978 in Hungary (1970s in Europe)
• Which disease smells sour?
• EFB (European foul brood) gives sour smell of the larvae
• Which disease causes a gondola like scale?
• SBV (Sacbrood virus) - cause a dried out larva with this shape
• How many days are needed for the larvae to develop from the egg?
• 3 days
• Write the tasks of the workers
- 1-2 days - cleaning of cells
- 3-5 days - feeding older larvae
- 6-11 days - feeding younger larvae
- 12-17 days - comb construction
- 18-21 days - gardening, defending function
- From 22nd day - foraging for pollen and nectar
• How many brood cells can be collected from 10 x 10 comb?
• 800 - 850 brood cells (300-350gram honey)
• Treatment and prevention of Nosemosis apium?
- Treatment: Fumagillin or other antibiotics and disinfection of the hives and combs (if no honey or pollen is present formalin or acetic acid can be used)
- Prevention: Move the colonies to a new place in the autumn, give sufficient syrup and disinfect the hives and combs
• When does Nosemosis occur?
• At the end of winter and in spring time
• List the names of the European and Indian honey bees?
- European: Apis mallifera
* Indian: Apis cerana
• What is the pathogen of chalk brood?
• Ascosphaera apis (Pericystis apis)
• Which are zoonotic?
• Stone brood disease: Aspergillus fumigates and A. flavus
• Write 4 names of drugs to treat Varroosis?
- Amitraz (Apivar)
- Cymazol (Apitol)
- Perizin
- Fluvalinat (Apistan)
- Flumetrin (Bayvarol)
- Formic acid - mite treatment in winter
• Diagnostic method of American Foulbrood?
- Inspection of the combs and the dead larvae
- Studying of scales in UV light
- When a dry scale is put in 6 drops of warm milk(74 degrees C) milk curdles within a minute (in healthy it takes 13 minutes)
- Staining of spores with methylene blue
- Cultivate the bacteria in Colombia agar and CO2
- Can also use PCR
• Two types of CPV (Chronic paralysis virus)?
- Type 1 = Isle of Wight disease
- Abnormal trembling of wings and body, dislocated wings (fail to fly), bloated abdomen due to distention of honey sac with liquid.
- Dysentry- die within a few days
- Type 2 = Black robbers (little blacks)
- Become hairless and appear dark/black and shiny
- Guard bees prevent them from entering hive
- Become trembly, flightless and soon die
• Write 2 secondary bacteria causing foul odour and sour smell?
- Bacterium eurydice
- Strep. faecalis (causes the most sour smell)
- Paenibacillus alvei - produce foul odour
- Bacillus laterosporus
• Write the name of wax moth
- Greater wax moth = Galleria melonella
- Lesser wax moth = Achrois grisella
- Mediterranean flour moth = Anagasta kuehniella
• What are the larval glands?
• Only have the spinning gland
• What is bee louse?
- Braula coeca
- It is a brown wingless fly, found on the bodies of workers and queens (occasionally drones as well)
- They take food from the mouth of bees
• What is the sac brood virus?
- Viral infection that infects the young larvae. Nurse bees that are infected will infect the larvae through the food.
- Larva can not shed their last skin, fluid accumulates between larva and skin, body colour changes from white to yellow and becomes dry = gondola shaped scale
• List 4 instrument for bee keeping?
- Hive - rectangular frames with combs
- Queen excluder - grids
- Extractor - centrifugate
- Uncapper fork - electrical heated knife
• Honey enzymes and where is it produced?
• Invertase, diastate. Produced in the pharyngeal gland
• What is milk test?
• A method to diagnose American foul brood: Put a dry scale into 6 drops of warm milk (76 degrees C) and check if it curdles. If it does within a minute = positive. Normally it takes 13 minutes
• Taxonomy of bees?
• Hymenoptera order —> Aculeata —> Apidae family —> Apis
• Which Varroa species live where?
- The Korean haplotype = Varroa destructor: Most common worldwide > Africa, Europe, Middle East, Asia America
- The Japan-Thailand haplotype: Varroa jacobsoni> Japan, Thailand, America
• What causes apimyiasis?
• Senotainia tricuspis
• What is Braula?
• B. coeca = Bee louse
• When is the cleansing flight and what is it for?
• It is right after winter, when the workers fly out from the hives to defecate (to prevent dirt inside the hive)
• What happens before the winter?
• The drones are expelled from the hive. The beekeepers will clean, feed sugar syrup, take a Varroa control and prepare for the winter.
• How many % of the Varroa mite survive the winter?
• Non of the males will survive, because they die right after mating
• What is the royal jelly?
- A milky-white secretion produced by the pharyngeal gland of workers made of combination of pollen and honey.
- This is fed to the young larvae and queen for superior growth and development
• What is American foul brood?
- One of the most dreaded bacterial bee diseases in the world as it is very contagious.
- Caused by Paenibacillus larvae.
- Has to be reported to the OIE
• What is aspergillosis caused by?
• Caused by Stone-brood which is caused by Aspergillus flavus and fumigatus
• What must be collected when suspecting poisoning of bees?
• 1 litre of dead bees (10.000 bees) and samples of plants in a 2-3 km radius
• What is used against malphigamoebosis?
• There are no effective treatments against it
• Where do the symptoms of Aschosphera apis (chalk brood) occur?
- Larva are first fluffy and swollen, taking the form of the cell and quickly covered by white cotton like mycelium
- The larvae mummify, harden, shrink and appear chalk-like.
- Head of larvae is unaffected and there is no smell from the dead larvae
• Life span of worker bees?
- Summer: 4-6 weeks
* Winter: 4-5 months
• Which organ is the target of nosemosis?
• It develops exclusively within the cells of the epithelium of the mid-gut
• Which occurs in Hungary, V.destructor or V. jacobsoni?
• V. destructor
• Small beehive beetle?
- Aethina tumida
- Light yellowish brown at first and becomes brown, dark brown and black at full maturity
- Covered with fine hair
- They put stress on the colonies
- Larvae are scavengers and eat live brood and honey, defecate in the honey & promote fermentation
• Which haplotype occurs in Hungary? Japanese or Korean?
• Korean type - Varoa destructor
• 2 genus of flies causing Apimyasis?
- Melaloncha ronnai: Brazil
* Rondaniooestrus apivorus: South Africa
• What is the result of European foul brood?
- Caused by Melissococcus plutonius
- Must be reported to OIE
- Larvae are susceptible at any age of their unsealed life and often have no symptoms
- Larvae appear pale yellow and then brown, become displaced and curled upwards in their cell and then die
- The dead larva can have foul or sour smell, but not always as it can depend on the bacteria
- The scales to not adhere to the cell wall and can be removed easily
• What is the result of American foul brood
- Cause by Paenibaccilus larvae
- Have to be reported to OIE
- Can occur any time of the year when brood is present
- The capping of the diseased cell become moist and darken, larva drawn down into mouth of the cell and it becomes concave.
- Brood turns chocolate brown and melt into a mass on the floor —> papular tongue protrudes from scale to centre of cell
- Fish-glue like smell
- Scale is very difficult to remove and can remain infective for up to 30 years
• What are the foul brood bacteria?
- Paenibaccilus larvae = Gram + and spore forming - AFV
* Melissococcus plutonius = Gram + but no spores - EFV
How to diagnose viral diseases?
- Cultivation in adult for antiserum production
- Agar gel diffusion test
- Elisa
- PCR
- Symptoms
• What is propolis?
- Brownish resinous material collected by bees from the bud of trees and used as cement in repairing and maintaining the hive.
- Also used to sterilise the cells after larvae, honey or pollen.
- Important for prevention of infection
- Functions as a natural defence and immune system of the hive
• Which bee is in Europe?
• Apis mellifera
• Which bacterium can be isolated from an uncapped dead larvae?
- Melissococcus plutonius (EFV)
- M. pluton and secondary organism —> larva dies before it is capped and found by the nursing bees
- Bacillus alvei - diseased larvae are capped and fail to pupa
• How many larval stages are the in the bee?
• 5 stages
• Which sex of mites infects the trachea of bee?
• The female - lays eggs and male appears 11-12 days later
• Which will be sick of Nosemosis?
- Adults only!
* Mainly the workers but also queen and drones can be affected
• What is the agent causing Nosemosis and what is the infective form of it?
• Nosema apis, N. ceranae - infective part is the spore
• How many breathing holes does the bee have?
• 10 pairs of spiracles
• How old are the larvae that can get sick of sacbrood?
• Larvae that are about 2 days old are most susceptible
• What will hatch from a haploid egg?
• Drones (unfertilised)
• What will hatch from diploid egg?
• Workers and queens (fertilised)
• To whom is the royal jelly given?
• Queen, queen larvae and eggs are also laid in it
• Name 2 glands of workers
- Pharyngeal gland
- Wax gland
- Submandibular gland
- Sting
• Describe the compound eye?
- 3 ocelli and 2 oculus compositus
* Drone has about 9500 while other have about 4500
• What are the tasks of 3-5 day old workers?
• Feeding older larvae
• Name 4 viral diseases?
- Chronic paralysis virus (CPV)
- Acute bee paralysis virus (ABPV)
- Sacbrood virus (SBV)
- Deformed wing virus (DWV)
- Cloudy wing virus (CWV)
• What is beewolf?
- Philantus triangulum: Wasp
* Females lie down their eggs on dead bees and larvae feed on the dead bees as bees cannot kill them
• What is stonebrood?
- Fungal infection by Aspergillus flavus and A. fumigates which causes aspergillosis.
- May cause respiratory diseases in animals, man (zoonotic) and birds
- Toxins can kill adult honey bees
- Fungi can multiply in adult bees