BB Flashcards
LIGHT SPIN RPM AND MINUTES
2300 RPM 3 MINUTES
HEAVY SPIN RPM AND MINUTES
3500 RPM 5 MINUTES
WHOLE BLOOD VOLUME
450ml
Storage temp of WB
2 to 6 degrees
Storage time of WB
35 days
Commonly used anticoagulant for whole blood
CPDA-1
WB must be infused in how may hours
2-4 hrs
Temp range for Transport: Pre processed whole blood
20 to 24 degrees
Storage time for Transport: Pre processed whole blood
Less than 8 hours
Temp range for Storage: pre/post processed whole blood
2-6 degrees
Storage time for Storage: pre/post processed whole blood
35 days
Temp range for Transport: Processed blood
2-10 degrees
Storage time for Transport: Processed blood
Less than 24 hours
Random Donor Platelet Shelf life
5 days
Involves the removal of whole blood into its major components and/or exchanging one or more of these components and then returning the remaining blood components to the individual
Apheresis
Random Donor Platelet must be transfused within
As soon as possible
Single Donor Platelet must be transfused within
2 hours
FFP must be thawed at what temp?
37 degrees
Indications for FFP
CCDHT
*Coagulation Factor Deficiency
*Coumadin reversal
*Dilation Coagulopathy
*Hemorrhage in liver disease
*Thrombotic Thrombocytopenia Purpura
Indications for Cryoprecipitate
Factor VIII Deficiency
Von Willebrand Disease
Fibrinogen and Factor VIII
FFP/CRYO max storage time for -20 to -24 degrees
3 months
FFP/CRYO max storage time for -25 to -29 degrees
6 months
FFP/CRYO max storage time for -30 to -39 degrees
1 year
FFP/CRYO max storage time for -40 to -64 degrees
2 years
FFP/CRYO max storage time for -65 degrees
7 years
Indicators for Cryosupernate
Deficiency of clotting factors
Burns
Substitute for Albumin, exchange in TTP, Warfarin reversal
Cryosupernate
PRBC must be transfused within:
2-4 hours
PRBC increases Hct by how many percent?
3%
PRBC increases Hgb by how many g/L?
1 g/L
g/L of Hgb needed for Symptomatic Anemia?
<6 g/L
g/L of Hgb needed for Chronic Anemia?
<7 g/L
g/L of Hgb needed for Acute Coronary Syndrome?
<8 g/L
g/L of Hgb needed for Surgery?
<9 g/L
Indications for Washed RBCs
*History of Febrile Transfusion Reaction
* Allergic Reactions
*IgA deficiency
Washed RBCs must be transfused within:
4 hours
2 hours ideal
Indications for Leukocyte-poor RBCs
Immunocompromised, long term RBC therapy
Purpose: to detect antibodies that may have been missed by the antibody screen because of the absence of the corresponding antigen or present of a dosing antibody
Crossmatching
Crossmatching consisting of mixing the patient’s serum with donor red cells
Major Crossmatching
Crossmatching consisting of mixing the patient’s red cell with donor’s serum
Minor Crossmatching
Gel technology is invented by
Dr. Yves Lapierre (1988)
Also known as Antiglobulin test
Direct Coomb’s Test
What antibodies are detected using DAT
IgG and C3
DAT Application
Detect circulating donor red cells that are sensitized with the recipient antibody
Investigation of Hemolytic Transfusion Reaction
DAT Application
To detect maternal antibodies that have crossed the placenta to sensitize fetal red cellsp
Investigation of Hemolytic Disease of the Fetus and Newborn
DAT Application
To detect autoantibody sensitizing a patient’s own red cells
Investigation of Autoimmune Hemolytic Anemia
DAT Application
To detect anti-drug/red cell antibodies and/or subsequent activation of the complement system
Investigation of drug-induced hemolysis
This test looks for free-flowing antibodies against certain red blood cells. It is most often done to determine if you may have a reaction to a blood transfusion.
Indirect Coomb’s Test
IAT Application
To detect clinically significant IgG alloantibodies in the recipient
Antibody Detection
IAT Application
To specifically identify those antibodies detected by reagent screening cells or by donor red cells
Antibody Idenfication
IAT Application
To detect antibodies that may have been missed by the antibody screen because of absence of the corresponding antigen or present of a dosing antibody
Crossmatching
IAT Application
To type patient or donor red cells for antigens that can be detected by IgG antisera reactive only by the AGT.
Red cell antigen typing
