BAsics Flashcards
An intricate network of microfilaments that extend throughout the cytoplasm
Cytoskeleton
Is cytoskeleton dynamic?
yes
Is cytosketon only bone?
No, it is also muscle
3 Imp. role of cytoskeleton
- Cell shape 2. Cells to organoze its internal compartments 3. cells to move
IF protein
Fibrous
MT
globular tibulin subunit
AF
Globular sctin
IF diameter
10nm
IF found?
Nuclear Lamina
I
IF basic str.
Rope like fiber
MT basic str
Hollow cylinder
MT diameter
25 nm
AF or microF diameter
7nm
AF basic str.
HELICAL POLYMER OF ACTIN
AF 4 LOCATIONS
MIcrovilli
contractile bundles in cytoplasm
filopodia
contractile ring
Cytoskeleton with polarity
MT
ACTIN FILAMENT
Actin monomers carry ATP Or GTP
ATP
MT carry ATP Or GTP
GTP
Treadmilling of Actin filament or MT?
ACTIN F.
of Actin filament or MT?
Dynamic instability
MT
Simultaneous gain of monomers at the plus end and loss at minus end
Treadmilling
Rapid switch from growth to shrinkage at the +end
Dynamic instability
drug that prevent actin polymerization
Cytochalasin
Latrunculin
drug that stabilize actin filament from depolarization
Phalloidin
Proteins that keep actin monomers from polymerizing
Thymosin
Profilin
A
Actin binding proteins that promote actin polymeerization
Formins
Actin Related Proteins ARPs
Monomeric GTPase family that regulate actin cytoskeleton
Rho protein
Toughest and most durable cytoskeletal filament
IF
CK that forms Nuclear Lamina
IF
CK that forms Desmosomes
IF
Covalent or non covalent int. btw IF
NON COVA
Terminal domains of IF same or diferent in different type of IF
Differeent in size and amino acid sequence
Class 1 and 2 IF
Acdic keratin
BAsic K.
Class 3 IF
DEsmin, GFAP,Vimentin
Class 4 IF
Neurofilaments
Class 5 IF
LAMINS
LAmins are Phosphorylated by which kinase
CDK
MT Subunits
alpha and beta
MT FUNCTION
- Transporting, positioning memb-bound organelles, within cell
- Guiding intracellulartransport of cytosolicmacromolecules
Disorder when nuclear lamin defect
Progeria
Rare class of premature aging
Paa movie
The tubulin dimer stack by what kind of interaction
Noncoavalent
MT has how many parallel protofilaments
13
Tubulin in = and - end of MT
PLUS BETA TUBULIN
MINUS ALPHA
MT grows at which end
+
MTOC in animal cell
Centrosome
Centrosome made of what kind of tubulin
GAmma
Starting point or nucleating dite for groth of one MT
Each gamma tubulin ring complex
Which end of MT is embedded in centrosome
-
When does centriole has a role as organising center for MT
cILIA ND fLAGELLA
BAsaal bodies
Beating of cilia is regulated by
Nexin
MT
Ck that position organelles in position
MT
Imp. Actin binding protein that promote actin polymerization
Formin