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H3MATOLOGY > BASIC HEMATOLOGICAL METHODS OF EXAMINATION > Flashcards

BASIC HEMATOLOGICAL METHODS OF EXAMINATION Flashcards

1
Q

Basic screening test and most frequently ordered laboratory procedure

A

CBC or Complete Blood Count

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2
Q

Measurement of the concentration of Hgb in the blood

A

Hemoglobin Determination

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3
Q

Used to screen for disease associated with anemia and to evaluate polycythemia

A

Hemoglobin Determination

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4
Q

test indirectly measures
the RBC mass

A

Hematocrit Determination

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5
Q

Results are expressed as the percentage by volume of packed RBCs

A

Hematocrit Determination

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6
Q

series of test that determines number , variety, percentage concentration and quality of blood cells

A

CBC

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7
Q

valuable diagnostic information about the hematologic and other body system , prognosis response to treatment and recovery

A

CBC

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8
Q

Hgb, Hct, RBC and WBC count , Differential Count

A

CBC

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9
Q

Important buffer in the extracellular fluid

A

Hemoglobin

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10
Q

–main component of erythrocytes

A

Hemoglobin

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11
Q

–serves as the vehicle for the transportation of oxygen and carbon dioxide

A

Hemoglobin

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12
Q

Ratio of the volume of packed RBCs to the volume of whole blood

A

Hematocrit or Packed Cell Volume

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13
Q

blood test used to find out how many RBCs

A

RBC COUNT or Erythrocyte Count

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14
Q

Increase RBC

A

Polycythemia Vera

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15
Q

Decrease RBC

A

Anemia

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16
Q

measures the number of WBC and may serve as guide to the severity of the disease process that detects hidden infection

A

WBC Count or Leukocyte Count

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17
Q

WBCs may be counted visually using a microscope and ____________

A

HEMACYTOMETER

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18
Q

WBC COUNT

dilution is _______ and the diluent is a dilute _______

A

1:20—acid solution

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19
Q

detect immature WBC and abnormalities

A

DIFFERENTIAL COUNT

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20
Q

total count of circulating WBC is differentiated according to the five types of leukocytes

A

NEBLM

21
Q

pyogenic infection

A

neutrophils

22
Q

Parasitic infection

A

Eosinophils and Basophils

23
Q

allergic disorder

A

Eosinophils

24
Q

Viral infection

A

Lymphocytes

25
Q

severe infection by phagocytosis

A

Monocytes

26
Q

Use to assess the erythropoietic activity of the bone marrow

A

Reticulocyte count

27
Q

Reticulocyte Count
–whole blood, anticoagulated with EDTA is stained with a supravital stain such as __________ or __________

A

new methylene blue—brilliant cresyl blue

28
Q

Rate at which red blood cells sediment in a period of one hour

A

Erythrocyte Sedimentation Rate (ESR)

29
Q

Common hematology test and nonspecific measure of inflammation

A

Erythrocyte Sedimentation Rate (ESR)

30
Q

___________ is one of the oldest hematology tests and detects inflammation and roughly estimates its intensity

A

ERYTHROCYTE SEDIMENTATION RATE (ESR)

31
Q

What test uses graduated concentrations of saline solutions to detect SPHEROCYTES (RBCs with proportionally reduced surface membrane area) in hereditary SPHEROCYTOSIS or WARM AUTOIMMUNE HEMOLYTIC ANEMIA?

A

OSMOTIC FRAGILITY TEST

32
Q

Osmotic fragility test uses graduated concentrations of saline solutions to detect ___________ (RBCs with proportionally reduced surface membrane area) in hereditary ___________

A

SPHEROCYTES—SPHEROCYTOSIS or WARM AUTOIMMUNE HEMOLYTIC ANEMIA.

33
Q

phenotypically detects an inherited RBC enzyme deficiency causing severe episodic hemolytic anemia

A

GLUCOSE-6-PHOSPHATE DEHYDROGENASE ASSAY

34
Q

Sickle cell solubility screening assay confirmatory TESTs

A
  1. HEMOGLOBIN ELECTROPHORESIS
  2. HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
35
Q

Detect and diagnose sickle cell anemia and other inherited qualitative hemoglobin abnormalities and thalassemia’s

A

SICKLE CELL SOLUBILITY SCREENING ASSAY or HEMOGLOBIN ELECTROPHORESIS and HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

36
Q

Platelet Parameters included in the complete blood count (CBC)

A

Platelet Count and Mean Platelet Volume MPV

37
Q

High WBC

A

leukocytosis

38
Q

Low WBC

A

Leukopenia

39
Q

increased RBC count

A

polycythemia vera

40
Q

decreased RBC and cause of malfunction in the bone marrow

A

ANEMIA

41
Q

2 Methods of Hgb for manual counting

A
  1. Cyanmethemoglobin method (HiCn)
  2. Sahli’s acid-hematin method
42
Q

True or False

Differential count can be done with chemotherapy patients

A

False—Can’t be done with chemotherapy patients since the treatment destroys their cells → 100 cells can’t be identified

43
Q

Healthy individuals WBC Count

A

100 or 5.75 x 10^9/L

44
Q

Whole blood, anticoagulated with EDTA is stained with a _________ that stains the living cells.

A

supravital stain

45
Q

Supravital stains used:

A
  1. New methylene blue
  2. Brilliant cresyl blue
  3. Pure azure blue
46
Q

disposable and most common method to measure the ESR

A

Westergren method

47
Q

Method used to measure the ESR and HCT

A

Wintrobe method

48
Q

Also known as sedrate

A

Erythrocyte Sedimentation Rate (ESR)

49
Q

Erythrocyte Sedimentation Rate (ESR) unit of measurement

A

mm/hr

H3MATOLOGY (2 decks)

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