Basic Flashcards
Bipolar bodies
Safety pin appearance in Wayson stain
Yersinia pestis
Tackhead bacillus
Drumstick/Lollipop appearance
Clostridium tetani
Tumbling motility in Hanging drop test
Listeria
Darting motility in Hanging drop test
Campylobacter
Gliding motility in Hanging drop test
Capnocytophaga
Causative agent of Tetanus/Lock jaw
Clostridium tetani
Toxin produced by Clostridium tetani that causes Tetanus and spasmodic contracions
Tetanospasmin (neurotoxin)
Causative agent of Botulism
Clostridium botulinum
Toxin produced by Clostridium botulinum that causes Botulism and flaccid paralysis
Botulinum toxin (neurotoxin)
Pink to purple colonies with green metallic sheen on EMB
Escherichia coli
Pink colonies with dark center on EMB
Fish eye colonies
Enterobacter
The following are H2S (+) organisms except: Salmonella Proteus Arizona Corynebacterium Edwardsiella
Corynebacterium
SPACEd Salmonella Proteus Arizona Clostridium Edwardsiella
Bulls eye colonies on CIN (Cefsulodin-Irgasan-Novobiocin)
Yersinia enterocolitica
Exhibit satellitism, dew drop, satellite colonies around S. aureus
Mousy or mouse net smell, musty basement odor
Haemophilus influenzae
Preferred medium for isolating Bordetella pertussis
Regan Lowe (enrichment and selective)
Medusa head colonies
Bacillus anthracis
Beaten egg consistency
Bamboo pole arrangement on culture
String of pearls on MHA with penicillin
Bacillus anthracis
Molar tooth appearance
Actinomyces
Fried egg appearance on culture
Lack cell wall
Smallest free living organism
Mycoplasma
No cell wall: Mycoplasma, Ureaplasma
G(+) bacilli, non sporeforming, nonpigmented tan to buff colonies that are dry, warty, granular and rough resembling cauliflowers
Mycobacterium tuberculosis
G(-) bacilli, obligate aerobe, grape like or corn tortilla like odor (production of 2-aminoacetaphenone) with serrated, confluent growth
Pseudomonas aeruginosa (swarming motility)
All enterobacteriaceae are aerogenic except:
Shigella
Biological indicator/Quality control in autoclave
Spores of Bacillus stearothermophilus
Biological indicator/Quality control in oven, dry heat sterilization
Spores of Bacillus subtilis var. niger
Biological indicator/Quality control in Ethylene Oxide (ETO)
Bacillus subtilis var. globigii
Biological indicator/Quality control in Ionizing radiation
Bacillus pumilis
Most abundant normal flora in a throat and nasopharyngeal specimen
Alpha hemolytic Strep: Viridans strep
Most common agent of UTI
Escherichia coli
Exhibits classic starbust pattern on gram stain
Resembles lymphocytes
Cryptococcus neoformans
G(+) cocci in grape like clusters
Creamy white pin head colonies on BAP
Staphylococcus
Beta zone of hemolysin on BAP
Staphylococcus aureus
Golden yellow growth on Loeffler’s Serum Slant (LSS)
Staphylococcus aureus
Lemon yellow growth on Loeffler’s Serum Slant (LSS)
Staphylococcus citreus
Porcelain white growth on Loeffler’s Serum Slant (LSS)
Staphylococcus albus/
Staphylococcus epidermidis
Flame/Bullet/Lancet shaped diplococci on Gram stain
Streptococcus pneumoniae
Causative agent of Lobar pneumonia with rusty sputum
Streptococcus pneumoniae
Alpha hemolytic on BAP
Causative agent of Subacute Bacterial Endocarditis (SBE)
Viridans streptococci
Viridans strep associated with dental carries
Streptococcus mutans
Nutritionally Variant Streptococci (NVS)
Need cysteine/pyridoxal (vit B6)
Abiotrophia spp.
G(-) diplococci resembling coffee beans or kidney beans
Neisseria sp. except N. elongata (rod shaped)
Causative agent of ophthalmia neonatorium in newborns
Neisseria gonorrhoeae
Causative agent of Waterhouse-Friderichsen syndrome: hemorrhage in adrenal gland
Neisseria meningitidis
Causative agent of Montezumas revenge, turista, travelers and childhood diarrhea
Profuse watery stool
Enterotoxigenic Escherichia coli (ETEC)
Causes a Shigella like infection A. ETEC B. EIEC C. EHEC D. Shigella
Enteroinvasive Escherichia coli (EIEC)
Friedlander’s bacillus
Klebsiella pneumoniae
Exhibits a rancid potato-like odor
DNAse, Gelatinase and Lipase (+)
Serratia odorifera
Causative agent of Enteric/Typhoid fever: Pea soup stool
Salmonella
Causative agent of Bacillary dysentery
Shigella dysenteriae (Shigellosis)
Non spore forming g(-) anaerobic rods, lysine deamination and MR (+)
Exhibit swarming motility
Proteus mirabilis (OX-K): putrid odor Proteus vulgaris (OX-2, OX-19)
Causative agent of Black death
Stalactite pattern on broth culture
Exhibits a Safety pin appearance due to bipolar bodies inclusion on Wayson stain
Yersinia pestis
Old sock odor on Mannitol Salt agar
Staphylococcus aureus
Freshly plowed field odor
Nocardia sp.
Causative agent of Swimmer’s ear: Mild otitis externa/media
Pseudomonas aeruginosa
Causative agent of Ecthyma gangrenosum: skin lesions
Hot tub/Whirlpool dermatitis
Pseudomonas aeruginosa
Causes respiratory infections in patients with cystic fibrosis
Burkholderia cepacia
Causative agent of Glanders disease: a disease of horses transmittable to humans
Burkholderia mallei
Causes mellioidosis: Glanders like
Whitmore’s bacillus
Vietnamese Time Bomb (can reactivate after 9years)
Burkholderia pseudomallei
Motile G(-) anaerobic bacilli, oxidase (+)
Produces Choleragen toxin causing Rice water stool
“Fish in the stream” appearance
Yellow colonies on TCBS (sucrose fermenter)
Vibrio cholerae
G(-) anaerobic comma shaped bacilli
Vibrio sp.
Pfeiffers bacillus
Causes meningitis in children <5 yrs old
Haemophilus influenzae
Formerly known as Koch weeks bacillus
Causative agent of Pink Eye Conjunctivitis
Haemophilus aegypticus
Smallest pathogenic bacilli
Infective agent of Soft chancre, chancroid
School of fish arrangement
Haemophilus ducreyi
Bacteria that can grow at 42-43C
Pseudomonas aeruginosa
Campylobacter
S-shaped G(-) bacilli resembling “Sea gulls wings” appearance associated with gastritis and diarrhea exhibiting darting motility by single polar flagella
Campylobacter
G(-) bacilli, optimum temp for growth at 35-37C, motile with 3-6 flagella
Associated with peptic ulcer
Helicobacter
Causative agent of Whooping cough
Mercury droplet colonies
Bordetella pertussis
Non motile, G(-) aerobic cocobacilli
Causative agent of Brucellosis, undulant fever, Malta fever
Brucella
Causative agent of Legionnaire’s disease, Pontiac fever
Legionella pneumophila
Causative agent of Pittsburgh pneumonia
Legionella micdadei
Wiga’s agent of pneumonia
Legionella bozemanni
Causes Shipping fever in cattles
Causative agent of Pasteurellosis
Pasteurella multocida
Obligateky aerobic non motile, faintly staining g(-) coccobacilli
Causes Tularemia
Francisella
Associated with bacterial vaginosis, clue cells in cytology/Paps
Fishy amine like odor in Whiff/Sniff test
Gardnerella vaginalis
G(-) non spore forming bacteria
Causative agent of:
-Rat bite fever: Spirillum minor: from animal bite
-Haverhill fever: from ingestion of contaminated milk
Streptobacillus moniliformis
G(-) capnophilic, fusiform or filamentous bacilli exhibiting gliding motility
Capnocytophaga
Pleomorphic, Encapsulated g(-) bacilli
Causative agent of Granuloma Inguinale or Donovanosis
Calymmatobacterium granulomatis
Corroding bacterium with characteristic smell of bleach on agar
Eikenella corrodens
Spore forming bacteria
Bacillus
Clostridium
Aerobic G(+) bacill, catalase (+) Causative agent of Cutaneous anthrax Pulmonary anthrax (Woolsorter's disease) Gastrointestinal anthrax
Bacillus anthracis
Causative agent of myonecrosis (gas gangrene) Exhibits double zone of hemolysis Reverse CAMP (+) Lecithinase (+) Nagler reaction (+)
Clostridium perfringens
Agent of food (canned goods), wound, and infant botulism “Floppy baby”
Clostridium botulinum
Causes spastic contractions and lock jaw
Clostridium tetani
Cause of antibiotic-associated pseudomembranuos colitis
(Clindamycin: 1st antibiotic)
Cultured on CCFA (Cylcoserine Cefoxitin Fructose Agar)
Clostridium difficile
Klebs-Loeffler bacillus
Corynebacterium diphtheriae
Club shaped, dumb bell shaped, Parallel, Palisading, Picket fence, Chinese character appearance
Corynebacterium sp,
Multidrug resistant diphthteroid associated with endocarditis, pneumonia, perotonitis
Corynebacterium jeikeium
Formerly group JK