Bailey Chapter 7 Flashcards
It requires complex, exceptional media components for growth.
Fastidious organism/bacteria
The process of growing microorganisms in culture and growing them in artificial environments.
Cultivation
What do you call the artificial environment where cultivation is processed?
in vitro environment
It is a solidfying agent added to nutrients and water
Agar
Agarose melts at ____ and resolidifies at ______.
Melts at 95°C and above.
Resolidifies at 50°C.
Nutrients dissolved in water
Broth
True or False: At least 20⁶ bacterial/mL of broth are needed for turbidity to be detected with the unaided eye.
False. At least 10⁶ bacterial/mL is needed.
It provides preliminary information regarding the presence or absence of a
pathogen.
Direct Gram Staining
Give the three identification methods
Phenotypic Method
Genotypic Method
Immunologic Method
Infection site during cultivation
in vivo environment
Cleavage of amine group from amino acid that occurs
in air.
Deamination
It is an anaerobic process that requires an acid environment for activation.
Decarboxylation
Moeller decarboxylase base contains:
Glucose
Amino Acid substrate
pH indicator
Organisms are inoculated in a tube overlaid with ______.
mineral oil
What is the color of the pH indicator from the acid of the bacteria in early incubation after which it utilizes glucose?
yellow
Organisms that can decarboxylate begins to attack substrate and produce the amine product which increases the pH:
Bromcresol: Yellow > _______
Phenol red: Yellow > _______
Bromcresol: Purple
Phenol red: Red
It is a combination medium for identification of
decarboxylation and deamination in a single tube.
Lysine iron agar
True or False: Lysine iron agar contains 0.1% dextrose.
True
It is capable of deaminating lysine in the presence of oxygen (RED SLANT).
Proteus spp
An ability of particles in suspension to refract and deflect light rays passing
through the suspension.
Turbidity
Match the following inhibitor to their respective Organisms:
Inhibitor:
- Susceptibility to optochin and solubility in bile.
- Ethanol survival
- Ability to hydrolyze esculin in the presence of bile
- Growth in the presence of various NaCl concentration.
Organism:
A. Enterococci spp. & Vibrio spp.
B. Streptococcus pneumoniae
C. Enterococci spp. In combination with NaCl
D. Bacillus spp.
- B.
- D
- C
- A
Place the letter in its appropriate number
Image Link: https://drive.google.com/file/d/1MMOpbp0ROAT2utR6tNT–v7NkxPn49Wr/view?usp=sharing
- E
- A
- H
- B
- G
- C
- F
- D
Bacteria that produce ______ are able to degrade amino acid tryptophan into pyruvic acid, ammonia and indole.
tryptophanase
It hydrolyzes the substrate urea into ammonia, water, and carbon
dioxide.
Urease
What range do clinically significant bacteria prefer?
6.5 - 7.5 pH or Near Neutral Range
Designed to optimize growth and differentiate a specific type of organism. Also used in the identification of yeast.
A. Chocolate agar
B. Chromogenic media
C. Blood agar
D. Loeffler medium
B
Enrichment and selective medium for isolation of Bordetella pertussis.
A. Schaedler agar
B. Trypticase soy broth
C. Regan Lowe
D. Thioglycollate broth
C
Isolation of Corynebacterium diptheriae.
A. Cystine-tellurite blood agar
B. Hektoen enteric agar
C. Loeffler medium
D. MacConkey agar
A
Supports growth of anaerobes, aerobes, microaerophilic, and fastidious microorganisms.
A. Xylose lysine dexoycholate agar
B. Brain-heart infusion agar
C. Blood agar
D. Thioglycollate broth
D
Intracellular nutrient released in Cell lysis
A. Hemin and Nicotinamide adenine dinucleotide
B. Neisseria gonorrhoeae and Haemophilus spp.
C. Colistin and Nalidixic acid
D. Salmonella and Shigella spp.
A
