Bailey Chapter 7 Flashcards
It requires complex, exceptional media components for growth.
Fastidious organism/bacteria
The process of growing microorganisms in culture and growing them in artificial environments.
Cultivation
What do you call the artificial environment where cultivation is processed?
in vitro environment
It is a solidfying agent added to nutrients and water
Agar
Agarose melts at ____ and resolidifies at ______.
Melts at 95°C and above.
Resolidifies at 50°C.
Nutrients dissolved in water
Broth
True or False: At least 20⁶ bacterial/mL of broth are needed for turbidity to be detected with the unaided eye.
False. At least 10⁶ bacterial/mL is needed.
It provides preliminary information regarding the presence or absence of a
pathogen.
Direct Gram Staining
Give the three identification methods
Phenotypic Method
Genotypic Method
Immunologic Method
Infection site during cultivation
in vivo environment
Cleavage of amine group from amino acid that occurs
in air.
Deamination
It is an anaerobic process that requires an acid environment for activation.
Decarboxylation
Moeller decarboxylase base contains:
Glucose
Amino Acid substrate
pH indicator
Organisms are inoculated in a tube overlaid with ______.
mineral oil
What is the color of the pH indicator from the acid of the bacteria in early incubation after which it utilizes glucose?
yellow
