bacteriology_lab_20231208052941 Flashcards
All forms of microbial life including spores and prions are killed
Sterilization
Most resistant microbial form
Prions
Inactive form of bacteria
Spores
All pathogenic microorganisms, not necessarily all microorganisms, are killed
Disinfection
Most common physical method of sterilization
Incineration
Temperature for incineration
870-980
Produced by incineration which are its drawback
Toxic fumes/metals
Also known as principle of steam under pressure
Moist heat
Physical method for heat stable objects
Moist heat
What equipment is used in moist heat
Autoclave
Most common type of autoclave
Gravity displacement
Temperature, time, and pressure for routine method of moist heat which is used for media, liquids, and instruments
121 degree C, 15 mins, 15 psi
Temperature and time for infectious medical wastes under moist heat
132 degree C for 30-60 mins
Fastest, simplest, and most effective method for physical sterilization
Moist heat
Bacillus steatothermphilus is the biological indicator for
Moist heat
Sealed ampules is used to ensure that this method for sterilization is working. What colors indicate if the spores are killed or not?
Moist heatKilled= purpleNot killed= yellow
Pressure sensitive autoclave tape will show black stripes when? (Sterilized or not)
Sterilized
This is the alternate heating, incubation, and heating under moist heat
Fractional
100°C for 30 mins for 3 consecutive days
Tyndallization
Thickening through evaporation
Inspissation
Inspissation temperature, and time
76°-80°C for 2 hrs for 3 days
This is used for glasswares, oils, and powders
Dry heat
Uses 160°-180° for 1.5-3 hrs
Dry heat
Biological indicator for dry heat
Bacillus subtilis var nigar
Technique used for dry heat (what instrument)
Wire loop
Physical sterilization for heat sensitive objects
Filtration
This physical sterilization method is used for antibiotic solutions, toxic chemicals, radioisotopes, vaccines, and carbohydrates
Filtration
